Relative concentration of Apple mosaic virus coat protein in different parts of apple tree

Svobodá, J.; Polák, J.

doi:10.17221/39/2009-hortsci

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…Diseases caused by the three latent viruses, ASPV, ASGV, and ACLSV, are usually symptomless (Nemeth, 1986) and ApMV was also reported to have no apparent symptoms in some apple cultivars (Svoboda and Polák, 2010), therefore, an accurate and sensitive method is needed to detect these viral infections. For routine diagnosis, fast and inexpensive are essential.…”

Section: Introductionmentioning

confidence: 99%

Multiplex RT-PCR detection and distribution of four apple viruses in China

Ji¹,

Zhao²,

Duan³

et al. 2013

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Apple trees are natural hosts of four economically important virus species in China. We used a simple, sensitive multiplex RT-PCR protocol with an internal control to simultaneously detect and differentiate four apple viruses: apple chlorotic leaf spot virus (ACLSV), apple mosaic virus (ApMV), apple stem pitting virus (ASPV), and apple stem grooving virus (ASGV). This multiplex RT-PCR could be used as an alternative to other routinely used detection methods. We used this protocol to evaluate the occurrence and distribution of the four apple viruses in China. The four viruses were widely distributed throughout the main apple production region of China, including Heilongjiang, Liaoning, Hebei, Beijing, Henan, Shanxi, Shaanxi, Shandong, Gansu, Ningxia, Xinjiang, Sichuan, and Yunnan. The four viruses, ApMV, ASPV, ASGV, and ACLSV, were present in 80.1%, 65.1%, 73.7%, and 69.7% of the samples, respectively. Two or more of the viruses were detected in most of the infections. The most frequent virus combinations were ApMV + ASGV + ASPV + ACLSV with an incidence of 27.22%, followed by ApMV + ASGV + ACLSV (14.37%), ApMV + ASGV + ASPV (12.54%), and ASGV + ApMV (11.01%). The incidence of the ASPV + ACLSV combination was the lowest (0.61%). This is the first extensive survey conducted in China for monitoring the four apple viruses, which provides important information for apple virus distribution and management in China.

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Section: Introductionmentioning

confidence: 99%

Multiplex RT-PCR detection and distribution of four apple viruses in China

Ji¹,

Zhao²,

Duan³

et al. 2013

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“…Another symptom related to pigment loss is variegation or breaking, in which flower petals show specks, lines, or sections of tissue with different colorations [60]. Yellowing in leaves, for example, is a characteristic symptom caused by tomato yellow leaf curl virus (TYLCV; Begomovirus, Geminiviridae) or CTV in tomato and citrus plants, respectively [133][134][135], whereas irregular light/dark green patterns are common symptoms of apple mosaic virus (ApMV; Ilavirus, Bromoviridae) in apple plants [136,137]. Viral factors such as the coat protein (CP) have been identified as triggering symptoms by interfering with chloroplast development [138][139][140][141][142].…”

Section: Beyond the Visible Symptoms Caused By Plant Viruses: Biochem...mentioning

confidence: 99%

“…If fruits are produced by these infected trees, they are colorless with a sour taste [227]. Studies conducted by Svoboda and Polák [136] showed that symptoms are more pronounced in spring, so they consider that the virus spreads easily in cold climates. ApMV is mainly distributed in Europe, Oceania, America, and some countries in Africa and Asia [137].…”

Section: Applesmentioning

confidence: 99%

Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective

Rodríguez‐Verástegui

Ramírez-Zavaleta²,

Capilla-Hernández³

et al. 2022

Plants

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Trees and herbs that produce fruits represent the most valuable agricultural food commodities in the world. However, the yield of these crops is not fully achieved due to biotic factors such as bacteria, fungi, and viruses. Viruses are capable of causing alterations in plant growth and development, thereby impacting the yield of their hosts significantly. In this work, we first compiled the world′s most comprehensive list of known edible fruits that fits our definition. Then, plant viruses infecting those trees and herbs that produce fruits with commercial importance in the global market were identified. The identified plant viruses belong to 30 families, most of them containing single-stranded RNA genomes. Importantly, we show the overall picture of the host range for some virus families following an evolutionary approach. Further, the current knowledge about plant-virus interactions, focusing on the main disorders they cause, as well as yield losses, is summarized. Additionally, since accurate diagnosis methods are of pivotal importance for viral diseases control, the current and emerging technologies for the detection of these plant pathogens are described. Finally, the most promising strategies employed to control viral diseases in the field are presented, focusing on solutions that are long-lasting.

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“…Titer of the virus was expressed as the last (highest) dilution of the sample, in which the sample was still positive. The virus titer is directly proportional to the concentration of the coat protein, which means that the relative concentration of the coat protein is a reciprocal of the virus titer (Svoboda and Polák, 2010).…”

Section: Semiquantitative Double Antibody Sandwich Elisa (Das-elisa)mentioning

confidence: 99%

Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch

Pavelková

Kiss

Nečas

2019

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Evaluation of the presence and concentration of PPV (Plum pox virus) in selected rootstocks was carried out in 2016 - 2017. For the purpose of the experiment we used rootstocks derived from crossbreeding of Prunus davidiana (Carr.) Franch, such as Cadaman and Barrier, and also a P. davidiana seedling. Peach seedling rootstock GF-305 was used as a control. The rootstocks were inoculated artificially with PPV strain M (Marcus). Both the rootstock and the inoculum were tested for presence of the virus by a serological method -semiquantitative DAS-ELISA test and molecular methods -RT-PCR, real-time RT-PCR and RT-LAMP. During the growing season the plants were evaluated for symptom intensity by using a scoring scale. The results show interdependency between symptom intensity and the amount of PPV in leaves, with DAS-ELISA test giving less positive samples than RT-PCR. The RT-LAMP and real-time RT-PCR methods were capable of revealing low concentrations of the virus even in symptom-free plants. The lowest PPV concentrations of all the four rootstocks were detected by real-time RT-PCR in P. davidiana. The highest PPV concentrations were detected in Barrier rootstock. In inocula, the lowest concentration was found in the inocula on Cadaman rootstock, whereas the highest PPV concentration was detected in the inocula inoculated on Barrier rootstock.

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Relative concentration of Apple mosaic virus coat protein in different parts of apple tree

Cited by 11 publications

References 6 publications

Multiplex RT-PCR detection and distribution of four apple viruses in China

Multiplex RT-PCR detection and distribution of four apple viruses in China

Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective

Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch

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