Relative biological effectiveness of pulsed and continuous 20MeV protons for micronucleus induction in 3D human reconstructed skin tissue

The Effectiveness of 20 MeV Protons at Nanosecond Pulse Lengths in Producing Chromosome Aberrations in Human-Hamster Hybrid Cells

“…These data are of high relevance for clinical use of laser-accelerated proton beams. Previous experiments on monolayer and 3D tissue cultures (in vitro) did not provide evidence for significantly altered radiobiological effectiveness in terms of cytogenetic damage or DNA repair (8)(9)(10)(11)(12). The RBE values determined for pulsed and continuous irradiation modes were always comparable to the RBE of 1.1, which is achieved by conventional proton therapy (20).…”

“…However, based on our previous in vitro experiments (8)(9)(10)(11)(12) we do not expect a relevant difference between pulsed and conventional proton irradiation.…”

“…With respect to these endpoints no indication for a significantly altered efficiency of pulsed protons compared to a continuous irradiation was observed (8)(9)(10)(11). Further biological experiments were performed using a human skin tissue model to account for the 3D geometry and the cell interaction, again without statistically significant changes in RBE according to dose rate (12). In addition to these in vitro studies, a technical set-up for in vivo irradiation of subcutaneous tumors in mice with pulsed and continuous proton beams has now been constructed at the SNAKE (13).…”

Water Radiolysis: Influence of Oxide Surfaces on H2 Production under Ionizing Radiation

“…Water radiolysis occurs in many situations (radiotherapy [1], radiosterilization [2], sewage treatment [3], food irradiation [4], etc.). In many cases, this water radiolysis is affected by solid/liquid interfaces.…”

New challenges in radiobiology research with microbeams

“…Three-dimensional models can reproduce many of the tissue characteristics in vivo (Griffith and Swartz 2006) and are therefore ideal targets in microbeams for studying non-targeted effects. So far, mostly skin constructs have been used at microbeams (Belyakov et al 2005;Sedelnikova et al 2007;Schmid et al 2010;Miller et al 2011), but new organotypic slice culture methods offer the possibility of irradiating parts from all kinds of human tissues and to study their late response (Merz et al 2010). The tissue models at microbeams can be very useful to clarify the role of cell signaling and tissue remodeling in radiation response.…”