Relative avidity of serum antibodies to putative periodontopathogens in periodontal disease

Abstract

Relative a1•idity of serum antibodies to putative periodontopathogens in periodontal disease. J Periodont Res 1993; 28: 444-450. •£) Munksgaard. 1993 ELISA was used to determine both the avidity and titre of lgG, lgA and IgM antibodies to the gram-negative anaerobe. Porphyromonas gingil"alis, in tvllenty periodontitis patients enrolled in a longitudinal study of attachment loss and eleven non-periodontitis affected subjects. The avidity and titre of lgG antibodies to Actinobacillus Actinomycetemcomitans were also examined. A crosssectional analysis of the longitudinal patients at baseline and non-periodontally affected controls confirmed earlier findings that IgG and lgA antibody titres to P. gingiva/is were higher in periodontitis patients than in individuals who were not periodontally affected. In this cross-sectional analysis, lgG antibody avidities to P. gingiva/is were not found to be significantly higher in periodontitis than in control subjects (p = 0.065). However, indications of the potential prognostic value of antibody avidity was demonstrated by the higher lgM avidities to P. gingimlis in patients who did not experience attachment loss during the three-month monitoring period than in those who did (p=0.0005).

Influence of periodontal disease, Porphyromonas gingivalis and cigarette smoking on systemic anti‐citrullinated peptide antibody titres

“…Antibody titres to P. gingivalis in the serum samples were ascertained as previously described (Mooney et al. ) with minor modification. Briefly, Dynotech Immunolon 1B microtitre plates (ThermoFisher, Loughborough, UK) were coated overnight with intact P. gingivalis NCTC 11834 (not fixed) at a density of 10 8 /ml in 100 mM bicarbonate buffer pH9.6.…”

IL-33 Exacerbates Periodontal Disease through Induction of RANKL

“…Antibody titers to P. gingivalis in the serum samples were ascertained as previously described (Mooney et al 1993) with minor modification. In brief, Dynotech Immunolon 1B microtiter plates (ThermoFisher, Leicestershire, UK) were coated overnight with heat-killed P. gingivalis W83 at an OD 600 0.02 in 100mM bicarbonate buffer (pH 9.6).…”

Strain‐dependent activation of the mouse immune response is correlated with Porphyromonas gingivalis‐induced experimental periodontitis

“…IgG subclasses and their avidity have also been implicated as a factor in periodontal disease progression. Mooney et al (1993) showed that patients who had not experienced attachment loss during a 3‐month monitoring period had anti‐ P. gingivalis antibodies with a higher avidity than those patients who experienced attachment loss. In periodontal disease subjects, it was found that a humoral response involving IgG2 may be ineffective in clearing P. gingivalis infections (Lamster et al 1998).…”