Relative amount of telomeric sequences in terminal villi does not differ between normal term placentas and placentas from patients with well-controlled type 1 diabetes mellitus

Zinková, Alžběta; Marová, Dominika; Koperdáková, Júlia; Mirchi, Tomáš P.; Korabečná, Marie; Jirkovská, Marie

doi:10.1016/j.placenta.2017.04.016

Cited by 6 publications

(8 citation statements)

References 15 publications

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“…In the same groups of patients, the immunohistochemically identified telomerase expression, the expression of mRNA for hTERT, and the expression of TERC gene copy number were lower in diabetic placentas [41]. On the other hand, no difference of mean telomere length was found in peripheral villi of normal placentas and placentas in well-controlled maternal diabetes examined by laser capture microdissection and qPCR [35], although the study on the same placental material discovered lower proliferative potential of cytotrophoblast and vascular endothelium of terminal villi in maternal diabetes [25]. Single telomere length analysis (STELA) was used for measurement of telomere length in normal placentas and placentas from gestational diabetes treated either by lifestyle intervention or by metformin or insulin therapy.…”

Section: Maternal Diabetes Mellitusmentioning

confidence: 79%

“…Telomere length analysis based on isolated DNA TRF [29][30][31] STELA [32][33][34] Q-PCR, T/S ratio [29,[35][36][37][38] Telomere length analysis based on examination of interphase nuclei…”

Section: Methods Referencesmentioning

confidence: 99%

“…Microdissection followed by qPCR allows selection of cells for analysis using classical histological technique and it is suitable for the studies based on archival material [35]. Microdissected cells may be examined by qPCR methodology, and therefore, its main disadvantage is that it provides information only about the relative average length of telomeres in analyzed samples.…”

Section: Methods Referencesmentioning

confidence: 99%

“…Maternal diabetes is associated with longer placental telomeres [38]; there is no difference in placental telomere length in well-controlled diabetes [34,35]; telomeres are shorter in placentas from poorly controlled diabetes [42] Lower expression of hTERT, hTERT-mRNA, lower TERC copy number in placentas from poorly controlled diabetes [41] Placenta associated with preeclampsia…”

Section: Normal Placentamentioning

confidence: 99%

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Telomeres and Telomerase Activity in the Human Placenta

Jirkovská¹,

Korabečná²,

Laššáková³

2020

Telomerase and Non-Telomerase Mechanisms of Telomere Maintenance

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Placenta is a transient organ ensuring the intrauterine development of the individual. To meet fetal requirements, rapid and continuous cell proliferation enlarges the areas of tissues maintaining maternofetal transport. The cell division in placenta is accompanied with shortening of telomeres leading to cell senescence. Telomerase activity, on the other hand, ensures replication of telomeres and allows the organ to serve till the end of pregnancy. This balanced process may be negatively influenced by unfavorable circumstances. Here, we summarize available data on telomere length as well as telomerase activity in placentas from normal and complicated pregnancies; attention is also paid to the comparison of methods used in relevant studies.

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Section: Maternal Diabetes Mellitusmentioning

confidence: 79%

“…Telomere length analysis based on isolated DNA TRF [29][30][31] STELA [32][33][34] Q-PCR, T/S ratio [29,[35][36][37][38] Telomere length analysis based on examination of interphase nuclei…”

Section: Methods Referencesmentioning

confidence: 99%

Section: Methods Referencesmentioning

confidence: 99%

Section: Normal Placentamentioning

confidence: 99%

See 2 more Smart Citations

Telomeres and Telomerase Activity in the Human Placenta

Jirkovská¹,

Korabečná²,

Laššáková³

2020

Telomerase and Non-Telomerase Mechanisms of Telomere Maintenance

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show abstract

“…40,41 Nevertheless, Zinková et al found no differences in TL between controls and well-controlled type 1 diabetes mellitus placental samples. 42 Although these studies have some methodological differences, [40][41][42] it suggests that a good disease management (leading to well-controlled diabetes) may prevent the detrimental effect of hyperglycemia over placenta aging.…”

Section: The Disturbance Of Telomere Dynamics In Fetal Membranes Contributes To Pregnancy Complicationsmentioning

confidence: 99%

Understanding the Role of Telomere Dynamics in Normal and Dysfunctional Human Reproduction

Arias-Sosa

2018

Reprod Sci

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In modern society, fertility problems and demand of treatment seem to be on the rise, which led to an increased interest in research regarding human reproduction. Among these efforts, the study of the molecular senescence process has gain notorious popularity as aging is one of the most important variables involved in reproductive capacity and since the comprehension of telomere dynamics has become an important and influential theme. This new knowledge regarding the reproductive aging process is expected to offer new tools to understand the acquisition, maintenance, and loss of fertility potential. Therefore, this review seeks to clarify the relevance of molecular aging (evaluated by telomere shortening) in human reproduction, showing that it is a dynamic and variable process modulated according to the specific tissue and stage of development. As well, it is discussed how telomere status influence the development and progression of some fertility pathologies, the outcome of assisted reproductive treatments, and programming of aging in the offspring.

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Telomere erosion as a placental clock: From placental pathologies to adverse pregnancy outcomes

Kohlrausch

Keefe

2020

Placenta

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Relative amount of telomeric sequences in terminal villi does not differ between normal term placentas and placentas from patients with well-controlled type 1 diabetes mellitus

Cited by 6 publications

References 15 publications

Telomeres and Telomerase Activity in the Human Placenta

Telomeres and Telomerase Activity in the Human Placenta

Understanding the Role of Telomere Dynamics in Normal and Dysfunctional Human Reproduction

Telomere erosion as a placental clock: From placental pathologies to adverse pregnancy outcomes

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