Relative abundance of tissue mRNA and association of the single nucleotide polymorphism of the goat NGF gene with prolificacy

Thomas, Naicy; Venkatachalapathy, R. Thirupathy; Aravindakshan, T. V.; Raghavan, K.C.; Mini, M.; Shyama, K.

doi:10.1016/j.anireprosci.2016.08.009

Cited by 11 publications

(4 citation statements)

References 24 publications

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“…In pigs, 17β-estradiol, one important reproductive hormone, was found to promote transcription of p53 [24]. Interestingly, the polymorphism of NGF, playing a role in augmenting folliculogenesis, has been reported to be associated with LS in both sheep and goats [17,[25][26][27].…”

Section: Candidate Genes Likely Functioning In Prolificacymentioning

confidence: 99%

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Lin

Wang

et al. 2021

Genes

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Ovine litter size (LS) is an important trait showing variability within breeds. It remains largely unknown whether inbreeding depression on LS exists based on genomic homozygous regions, and whether the homozygous regions resulted from inbreeding are significantly associated with LS in sheep. We here reanalyze a set of single nucleotide polymorphism (SNP) chip of six breeds to characterize the patterns of runs of homozygosity (ROH), to evaluate inbreeding levels and inbreeding depressions on LS, and to identify candidate homozygous regions responsible for LS. Consequently, unique ROH patterns were observed among six sheep populations. Inbreeding depression on LS was only found in Hu sheep, where a significant reduction of 0.016, 0.02, and 0.02 per 1% elevated inbreeding FROH4–8, FROH>8 and the total inbreeding measure was observed, respectively. Nine significantly homozygous regions were found for LS in Hu sheep, where some promising genes for LS possibly via regulation of the development of oocytes (NGF, AKT1, and SYCP1), fertilization (SPAG17, MORC1, TDRD9, ZFYVE21, ADGRB3, and CKB), embryo implantation (PPP1R13B, INF2, and VANGL1) and development (DPPA2, DPPA4, CDCA4, CSDE1, and ADSSL1), and reproductive health (NRG3, BAG5, CKB, and XRCC3) were identified. These results from the present study would provide insights into the genetic management and complementary understandings of LS in sheep.

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Section: Candidate Genes Likely Functioning In Prolificacymentioning

confidence: 99%

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Lin

Wang

et al. 2021

Genes

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“…However, reproductive traits are determined by complex regulatory factors, including the genetic material, nutrient levels, feeding environment, and management, etc. Genetic factors are more stable and easier to select than the more volatile nutrition and environmental factors in regulating goats’ fertility process [5,6,7]. The last decade has witnessed the development of the molecular genetic breeding method in livestock, such as C657T and G749C mutations in the Pregnancy-Associated Glycoprotein gene family ( PAGs ), which has significantly increased the number of live-born piglets ( p ≤ 0.05) in Hirschmann hybrid-line sows [8].…”

Section: Introductionmentioning

confidence: 99%

Genetic Effects of Single Nucleotide Polymorphisms in the Goat GDF9 Gene on Prolificacy: True or False Positive?

Wang

Yang

Zhang

et al. 2019

Animals

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Simple SummaryAs an important regulator factor, which was secreted by female oocytes, the growth differentiation factor 9 (GDF9) plays an essential role during the growth and differentiation of ovarian follicles. Single nucleotide polymorphisms (SNPs) within the GDF9 gene have been found to be involved in reproductive traits in livestock, and some of these mutations have been used as the effective makers in animal molecular breeding. However, it is remarkable that the SNPs of the goat GDF9 gene have not been systematically sorted and analyzed from the reported studies, which leads to an inability to find effective loci that could be applied in improving the prolificacy of goats via the molecular breeding method. In this study, we gathered and sorted 45 SNPs of the goat GDF9 gene from all relevant studies and the National Center for Biotechnology Information Search database (NCBI), and especially analyzed and discussed the relationship between part controversial and potentially effective SNPs and the reproductive traits. The results indicated that non-synonymous SNPs A240V, Q320P, and V397I and synonymous SNPs L61L, N121N, and L141L were six “true” positive SNPs in improving goat fertility. Nevertheless, the regulation pathways and the specific mechanism of these six SNPs on goat fecundity are not clear, which still need further study in more goat breeds and a large sample size. These results provided an effective tool for follow-up research studies on the molecular genetic breeding of goats’ reproductive traits.AbstractGoat reproductive traits are complex quantitative traits controlled by polygenes and multipoint. To date, some high-fertility candidate genes in livestock have been unearthed and the growth differentiation factor 9 (GDF9) gene is one of them, which plays a crucial role in early folliculogenesis. According to the relevant previous studies and the National Center for Biotechnology Information Search database (NCBI), a total of 45 single nucleotide polymorphisms (SNPs) have been detected in the goat GDF9 gene, but which one or which ones have important effects on goat fecundity is still uncertain. Hence, in order to find effective molecular markers for goat genetic breeding and accelerate the goat improvement, this study summarized and classified the above 45 SNPs into four kinds, as well as compared and analyzed the same SNP effects and the different SNPs linkage effects on the reproductive traits in different goat breeds. Since there were many SNPs in the goat GDF9 gene, only 15 SNPs have been identified in more than 30 goat breeds worldwide and they showed different effects on the litter size. Therefore, this study mainly chose these 15 SNPs and discussed their relationship with goat productivity. Results showed that three non-synonymous SNPs A240V, Q320P, and V397I and three synonymous ones L61L, N121N, and L141L played a “true” role in the litter size trait in many goat breeds around the world. However, the regulatory mechanisms still need further research. These results provide an effective tool ...

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“…This breed is generally considered to be low prolific. This breed has a lesser frequency of multiple births (Naicy et al, 2016). Improvement in the reproductive traits was very much essential due to the increased prolificacy of the goats that will directly enhance the income of the goat rearing farmers.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability Analysis of Early Growth Response 2 (EGR2) Gene in Native Goat Breeds of Kerala

Desai¹,

Naicy²,

Aravindakshan³

et al. 2020

IJAR

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Background: India is fortunate to have a vast livestock resource with the availability of 28 well defined goat breeds. Kerala is the home for two breeds of goats namely Malabari and Attapady Black. Due to high prolificacy and income through lower input goat rearing had attracted numerous farmers. Selection with the aid of molecular markers associated with production traits plays an essential role in goat breeding programmes. The EGR2 gene is a part of multigene family which encodes Cys2His2 type zinc-finger proteins which is responsible for DNA binding. This gene has a major role in cellular prolification, reproduction, proper growth and development ovarian follicles. Thus present study was conducted with an objective to detect single nucleotide variations of Early Growth Response 2 gene in native goat breeds of Kerala. Methods: This research was conducted in 153 Malabari goats and 129 Attappady Black goats from six centers viz., University Goat and Sheep farm Mannuthy and 5 field centers of ICAR- All India Coordinated Research Project on Goat Improvement. Genomic DNA was isolated and PCR was performed to amplify Exon 1, Exon 2 and 5 fragments of Exon 3 regions of Early Growth Response 2 gene. Single stranded conformation polymorphism (SSCP) technique was performed to detect Single Nucleotide Polymorphism (SNPs). Result: The SSCP revealed similar banding patterns and sequencing did not indicate any nucleotide variations in all the exons screened suggesting that EGR2 gene is highly conserved in native goat breeds of Kerala. This is the first study conducted to characterize EGR2 gene in goats making the current study a novel one.

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Relative abundance of tissue mRNA and association of the single nucleotide polymorphism of the goat NGF gene with prolificacy

Cited by 11 publications

References 24 publications

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Litter Size of Sheep (Ovis aries): Inbreeding Depression and Homozygous Regions

Genetic Effects of Single Nucleotide Polymorphisms in the Goat GDF9 Gene on Prolificacy: True or False Positive?

Genetic Variability Analysis of Early Growth Response 2 (EGR2) Gene in Native Goat Breeds of Kerala

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