Relationships between muscle mitochondrial metabolism and stress-induced corticosterone variations in rats

Duclos, Martine; Martin, Cyril; Malgat, Monique; Mazat, Jean‐Pierre; Chaouloff, Francis; Mormède, Pierre; Letellier, Thierry

doi:10.1007/s004240100675

Cited by 18 publications

(19 citation statements)

References 33 publications

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“…This is also true for Fischer rats, albeit less marked for the 50-mg pellet. It is important to note that the plasma corticosterone values of the ADX ϩ 200-mg pellets remained in the range of plasma corticosterone observed in chronically stressed rats (9), suggesting that the range of corticosterone concentrations achieved in the present experiment represents reference values ranging from low HPA activity to high (stress-induced) HPA axis activity.…”

Section: Methodological Considerationssupporting

confidence: 50%

“…have been used in these studies (21,22,35). To determine the effects of endogenous increases in plasma corticosterone, the natural GC in rat, on muscle mitochondrial metabolism, in a previous study (9) we submitted rats to 8 wk of daily stress (novel environment or forced exercise), and we demonstrated that plasma corticosterone concentrations were negatively correlated with mitochondrial metabolism. Although such correlations do not necessarily indicate a direct effect of the change in corticosterone concentration on mitochondrial function, this does suggest that plasma corticosterone and functional muscle mitochondrial amount are related.…”

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confidence: 99%

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Effects of corticosterone on muscle mitochondria identifying different sensitivity to glucocorticoids in Lewis and Fischer rats

Duclos

Gouarné²,

Martin³

et al. 2004

American Journal of Physiology-Endocrinology and Metabolism

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Duclos, Martine, Caroline Gouarne, Cyril Martin, Christophe Rocher, Pierre Mormède, and Thierry Letellier. Effects of corticosterone on muscle mitochondria identifying different sensitivity to glucocorticoids in Lewis and Fischer rats. Am J Physiol Endocrinol Metab 286: E159-E167, 2004. First published September 9, 2003 10.1152/ajpendo.00281.2003.-Previous studies in rat have demonstrated decreased number of mitochondria and uncoupling of oxidative phosphorylation after administration of glucocorticoids but at supraphysiological doses and using synthetic glucocorticoids. To analyze the relationships between corticosterone levels (the natural glucocorticoid in rat) and muscle mitochondrial metabolism, Lewis and Fischer 344 rats were bilaterally adrenalectomized and implanted with different corticosterone pellets (0, 12, 50, 100, and 200 mg of corticosterone). Rats bearing a corticosterone pellet delivering corticosterone at concentrations in the range of chronic stress-induced levels presented a lower amount of functional muscle mitochondria with a decrease in cytochrome c oxidase and citrate synthase activities and a depletion of mitochondrial DNA. Moreover, a strain difference in tissue sensitivity to corticosterone was depicted both in end-organ sensitive to glucocorticoids (body, thymus, and adrenal weights) and in muscle mitochondrial metabolism (Lewis Ͼ Fischer). Interestingly, this strain difference was also observed in the absence of corticosterone, with a deleterious effect on muscle mitochondrial metabolism in Fischer rats, whereas no effects were observed in Lewis rats. We therefore postulate that corticosterone is necessary for muscle mitochondrial metabolism exerting its effects in Fischer rats with an inverted U curve, whereby too little (only Fischer) or too much (Fischer and Lewis) corticosterone is deleterious to muscle mitochondrial metabolism. In conclusion, we propose a general model of coordinate regulation of mitochondrial energetic metabolism by glucocorticoids. skeletal muscle; cytochrome c oxidase; tissue sensitivity to glucocorticoids GLUCOCORTICOIDS (GC), hormones released by the adrenal cortex, have numerous homeostatic and stress-response functions. A delicate balance exists between the protective effects of adrenal steroids secreted in response to stressful experiences and the negative consequences that GC may have for many processes (20). For example, in skeletal muscles, GC insufficiency leads to debilitating fatigue, myalgia, and general muscle weakness (27), whereas excess GC causes muscular atrophy and a decreased rate of muscle protein synthesis (2). Among several mechanisms leading to muscle wasting in GC-induced myopathy, defects in mitochondrial function appear to be involved (21,22,35). With the administration of supraphysiological doses of GC in rat, electron microscopic and biochemical studies have demonstrated enlarged mitochondrial volume, decreased number of mitochondria, decreased substrate utilization, and uncoupling of oxidative phosphorylation (21). Studies of the...

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Section: Methodological Considerationssupporting

confidence: 50%

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confidence: 99%

Effects of corticosterone on muscle mitochondria identifying different sensitivity to glucocorticoids in Lewis and Fischer rats

Duclos

Gouarné²,

Martin³

et al. 2004

American Journal of Physiology-Endocrinology and Metabolism

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“…The Ciz1 gene may be complexly regulated by various transcription factors. As body exercise and stress induce glucocorticoids (15), consuming beans, including lentils, and exercising may have preventative effects for certain types of breast cancer. More studies, including in vivo experiments, need to be undertaken to elucidate the molecular mechanisms of glucocorticoids and tyrosine kinase inhibitors.…”

Section: Resultsmentioning

confidence: 99%

Clobetasol synergistically diminishes Ciz1 expression with genistein in U937 cells

et al. 2011

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Abstract. Cip-interacting zinc finger protein 1 (Ciz1) stimulates DNA replication and has been implicated in the tumorigenesis of breast cancer cells. In order to investigate the possibility of using medicinal glucocorticoids against breast cancer, we studied whether certain glucocorticoids affect the expression of Ciz1. The in vitro effect of clobetasol treatment on the reduction of Ciz1 expression was detected by reverse transcriptase-polymerase chain reaction. Western blotting also confirmed the down-regulation of the protein in a dose-dependent manner upon clobetasol treatment in U937 monocytoid cells. Furthermore, we found that Ciz1 protein expression was decreased after pre-treatment of the cells with clobetasol and genistein. An extract of Lens culinaris also had a synergistic effect on the repression of Ciz1 protein expression. IntroductionThe pathways by which cell proliferation is regulated during differentiation have yet to be established, therefore proteins that play a role in DNA replication are of considerable interest. Cip-interacting zinc finger protein 1 (Ciz1) stimulates DNA replication depending on the estrogen receptor (ER) and participates in the regulation of the cell cycle by increasing cdk2 kinase activity and inducing G1-S transition (1). Ciz1 protein regulates the ER by enhancing its transactivation activity and recruitment to target gene chromatin. Hypersensitivity to estrogen has been observed in patients with breast cancer (2). Ciz1 induces such a hypersensitivity to estrogen in breast cancer cells and induces the expression of cyclin D1, a target gene of ER. Overexpression of Ciz1 promoted the cell growth rate, anchorage independence and tumorigenesis of breast cancer cells (1). Conversely, repression of Ciz1 expression is expected to have interference effects on breast cancer progression. In fact, depletion of Ciz1 from cancer cells restrains entry to the S phase and inhibits cell proliferation. Ciz1 has also been found to be a glucocorticoid receptor (GR)-regulated gene (3).Glucocorticoids play an essential role in embryonic and cancer development (4,5). Due to their wide spectrum of activity and pro-apoptotic properties, glucocorticoids are some of the most commonly used drugs in hematological malignancies and are also used as chemotherapy regimens for, among others, solid cancer treatment. GR is a member of the nuclear hormone receptor family. Hormone-activated GR binds to glucocorticoid responsive elements (GRE) near target genes. In breast cancer, glucocorticoids, through their receptor, may interact with ER in a feedback loop regulating the activities of each other (6). It is known that glucocorticoids are capable of playing a complex role in breast cancer epidemiology, biology and treatment. We recently found the dose-dependent down-regulation of the Ciz1 protein upon genistein treatment in Daudi lymphoid cells (7). We, therefore, hypothesized that glucocorticoids and certain isoflavones may synergistically affect the expression of Ciz1. Materials and methodsCell culture. Th...

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“…27,36,43 Wheel running in mice was shown to increase muscle mass (TA and Sol), muscle mass/BW ratio and the COx activity in soleus. Because soleus is an oxidative muscle, 44 the increase in COx activity in the soleus implies that the wheel running was responsible for enhancing the efficiency of the COx activity and consequently the oxidative capacity, 30 but not the amount of functional mitochondria. Indeed, CS and SDH, Krebs cycle enzymes which reflect the amount of functional mitochondria, did not increase.…”

Section: Discussionmentioning

confidence: 99%

“…30 All the enzymatic activities were measured with a spectrophotometer (InfiniteM200; Tecan, Männedorf, Switzerland) at 378C and expressed in nanomoles of product formed per minute and per milligram of protein.…”

Section: Sdh Activitymentioning

confidence: 99%

In vivo cardiac anatomical and functional effects of wheel running in mice by magnetic resonance imaging

Aufradet¹,

Bessaad

Alsaid

et al. 2012

Exp Biol Med (Maywood)

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Physical activity is frequently used as a strategy to decrease pathogenesis and improve outcomes in chronic pathologies such as metabolic or cardiac diseases. In mice, it has been shown that voluntary wheel running (VWR) could induce an aerobic training effect and may provide a means of exploring the relationship between physical activity and the progression of pathology, or the effect of a drug on locomotor activity. To the best of our knowledge, in vivo magnetic resonance imaging (MRI) and other non-invasive methods had not been investigated for training evaluation in mice; therefore, it was proposed to test an MRI method coupled with a cardiorespiratory gating system on C57Bl/6 mice for in vivo heart anatomical and functional characterization in both trained and untrained animals. Twenty mice were either assigned to a 12-week VWR program or to a control group (CON - no wheel in the cage). At week 12, MRI scans showed an increase in the left ventricular (LV) wall mass in the VWR group compared with the CON group. The ex vivo measurements also found an increase in the heart and LV weight, as well as an increase in oxidative enzyme activities (i.e. cytochrome c oxidase [COx] in the soleus). In addition, correlations have been observed between ex vivo LV/body weight ratio, COx activity in the soleus and in vivo MRI LV wall mass/body weight. In conclusion, mouse cardiac MRI methods coupled with a cardio-respiratory gating system are sufficiently effective and feasible for non-invasive, training-induced heart hypertrophy characterization, and may be used for longitudinal training level follow-up in mouse models of cardiovascular and metabolic diseases.

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Relationships between muscle mitochondrial metabolism and stress-induced corticosterone variations in rats

Cited by 18 publications

References 33 publications

Effects of corticosterone on muscle mitochondria identifying different sensitivity to glucocorticoids in Lewis and Fischer rats

Effects of corticosterone on muscle mitochondria identifying different sensitivity to glucocorticoids in Lewis and Fischer rats

Clobetasol synergistically diminishes Ciz1 expression with genistein in U937 cells

In vivo cardiac anatomical and functional effects of wheel running in mice by magnetic resonance imaging

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