Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

Vokurková, M.; Nováková, Olga; Dobešová, Z; Kuneš, Jaroslav; Zicha, Josef

doi:10.1016/j.lfs.2005.03.014

Cited by 13 publications

(6 citation statements)

References 44 publications

(55 reference statements)

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“…Our previous study performed in salt hypertensive Dahl rats (Vokurková et al 2005) demonstrated similar dependence of particular ion transport parameters on mean cell hemoglobin content as our present study, suggesting the important contribution of immature cells to ion transport abnormalities reported in salt hypertensive animals. Less clear is the contribution of phospholipid alterations because phosphatidylserines had a major impact in this study, whereas sphingomyelins were important in our previous study of Vokurková et al (2005).…”

Section: Discussionsupporting

confidence: 90%

“…Our previous study in Dahl rats (Zicha and Duhm 1990) reported decreased MCHC and very high Na + -K + pump activity due to the augmented Na + leak in a subgroup of anemic salt hypertensive animals. Furthermore, we showed significant relationships between erythrocyte ion transport and membrane lipid composition in Dahl rats with salt hypertension in which MCHC correlated negatively with activity of the Na + -K + pump and Na + leak and positively with activity of the Na + -K + cotransport (Vokurková et al 2005).…”

Section: Introductionmentioning

confidence: 81%

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The Influence of Erythrocyte Maturity on Ion Transport and Membrane Lipid Composition in the Rat

Vokurková

Rauchová²,

Dobešová³

et al. 2016

Physiol Res

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Significant relationships between ion transport and membrane lipid composition (cholesterol, total phospholipids and sphingomyelins) were found in erythrocytes of salt hypertensive Dahl rats. In these animals mean cellular hemoglobin content correlated negatively with Na+-K+ pump activity and Na+ leak but positively with Na+-K+ cotransport activity. Immature erythrocytes exhibit lower mean cellular hemoglobin content (MCHC) than mature ones. The aim of the present study was to find a relationship between erythrocyte maturity, membrane lipid composition and ion transport activity in Wistar rats aged three months which were subjected to repeated hemorrhage (blood loss 2 ml/day for 6 days) to enrich circulating erythrocytes with immature forms. Immature and mature erythrocyte fractions in control and hemorrhaged rats were separated by repeated centrifugation. Hemorrhaged rats had increased number of reticulocytes but reduced hematocrit and MCHC compared to control rats. Immature erythrocytes of hemorrhaged rats differed from mature ones of control animals by elevated Na+-K+ pump activity, reduced Na+-K+ cotransport activity and increased Rb+ leak. These ion transport changes in immature erythrocytes were accompanied by higher concentration of total phospholipids in their cell membranes. Membrane phospholipid content correlated positively with Na+-K+ pump activity and cation leaks but negatively with Na+-K+ cotransport activity. Moreover, they were also negatively related with MCHC which correlated negatively with Na+-K+ pump activity and Rb+ leak but positively with Na+-K+ cotransport activity. Thus certain abnormalities of erythrocyte ion transport and membrane lipid composition detected in hypertensive animals might be caused by higher incidence of immature cells.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 81%

The Influence of Erythrocyte Maturity on Ion Transport and Membrane Lipid Composition in the Rat

Vokurková

Rauchová²,

Dobešová³

et al. 2016

Physiol Res

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“…The lipid extraction was performed with chloroform:isopropanol (14:11), according to the method of Oaklanderand Rose [ 34 ] and Vokurková, Nováková et al [ 35 ]. After phase separation, the organic layer was dried and re-suspended in a known volume of chloroform.…”

Section: Methodsmentioning

confidence: 99%

Effects of Iron Overload on the Activity of Na,K-ATPase and Lipid Profile of the Human Erythrocyte Membrane

et al. 2015

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Iron is an essential chemical element for human life. However, in some pathological conditions, such as hereditary hemochromatosis type 1 (HH1), iron overload induces the production of reactive oxygen species that may lead to lipid peroxidation and a change in the plasma-membrane lipid profile. In this study, we investigated whether iron overload interferes with the Na,K-ATPase activity of the plasma membrane by studying erythrocytes that were obtained from the whole blood of patients suffering from iron overload. Additionally, we treated erythrocytes of normal subjects with 0.8 mM H2O2 and 1 μM FeCl3 for 24 h. We then analyzed the lipid profile, lipid peroxidation and Na,K-ATPase activity of plasma membranes derived from these cells. Iron overload was more frequent in men (87.5%) than in women and was associated with an increase (446%) in lipid peroxidation, as indicated by the amount of the thiobarbituric acid reactive substances (TBARS) and an increase (327%) in the Na,K-ATPase activity in the plasma membrane of erythrocytes. Erythrocytes treated with 1 μM FeCl3 for 24 h showed an increase (132%) in the Na,K-ATPase activity but no change in the TBARS levels. Iron treatment also decreased the cholesterol and phospholipid content of the erythrocyte membranes and similar decreases were observed in iron overload patients. In contrast, erythrocytes treated with 0.8 mM H2O2 for 24 h showed no change in the measured parameters. These results indicate that erythrocytes from patients with iron overload exhibit higher Na,K-ATPase activity compared with normal subjects and that this effect is specifically associated with altered iron levels.

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“…Reduced NKA activity has been observed in erythrocytes from spontaneously hypertensive rats [20] and from human patients with essential hypertension [21], and claimed to be the major reason for Na ? accumulation in various cells [22,23]. Increased Na ?…”

Section: Introductionmentioning

confidence: 99%

Tubulin pools in human erythrocytes: altered distribution in hypertensive patients affects Na+, K+-ATPase activity

Amaiden

Santander

Monesterolo

et al. 2010

Cell. Mol. Life Sci.

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The presence of tubulin in human erythrocytes was demonstrated using five different antibodies. Tubulin was distributed among three operationally distinguishable pools: membrane, sedimentable structure and soluble fraction. It is known that in erythrocytes from hypertensive subjects (HS), the Na(+), K(+)-ATPase (NKA) activity is partially inhibited as compared with erythrocytes from normal subjects (NS). In erythrocytes from HS the membrane tubulin pool is increased by ~150%. NKA was found to be forming a complex with acetylated tubulin that results in inhibition of enzymes. This complex was also increased in erythrocytes from HS. Treatment of erythrocytes from HS with nocodazol caused a decrease of acetylated tubulin in the membrane and stimulation of NKA activity, whereas taxol treatment on erythrocytes from NS had the opposite effect. These results suggest that, in erythrocytes from HS, tubulin was translocated to the membrane, where it associated with NKA with the consequent enzyme inhibition.

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Relationships between membrane lipids and ion transport in red blood cells of Dahl rats

Cited by 13 publications

References 44 publications

The Influence of Erythrocyte Maturity on Ion Transport and Membrane Lipid Composition in the Rat

The Influence of Erythrocyte Maturity on Ion Transport and Membrane Lipid Composition in the Rat

Effects of Iron Overload on the Activity of Na,K-ATPase and Lipid Profile of the Human Erythrocyte Membrane

Tubulin pools in human erythrocytes: altered distribution in hypertensive patients affects Na+, K+-ATPase activity

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