The Influence of Erythrocyte Maturity on Ion Transport and Membrane Lipid Composition in the Rat

Vokurková, M.; Rauchová, Hana; Dobešová, Z; Loukotová, J.; Nováková, Olga; Kuneš, Jaroslav; Zicha, Josef

doi:10.33549/physiolres.933326

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…Red blood cell (RBC) damage is a recognized feature of hypertensive patients, including reduced RBC membrane fluidity 1 and impaired RBC deformability 2,3 . The reduced RBC membrane fluidity might participate in the pathogenesis of hypertension 4 . However, few direct evidence verified a causal relationship between RBC-related markers for hypertension's prevalence or long-term prognosis.…”

Section: Introductionmentioning

confidence: 99%

Hemoglobin specific volume width promotes the prevalence and poor long-term prognosis of American adult hypertensive patients: the NHANES 1999-2020

Zhang,

Chang,

Wang

et al. 2023

Preprint

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Background: Hypertensive patients are always accompanied by erythrocyte dysfunction. However, current erythrocyte-related indicators can't explain hypertension's prevalence and long-term prognosis. Therefore, hemoglobin specific volume width (HSW) was first created to explain this phenomenon. Methods: 59,867 adult participants from National Health and Nutrition Examination Survey (NMAHES) were included. HSW's quartiles were determined with Q1 [1.88,3.64] cL/g, Q2 (3.64,3.84] cL/g , Q3 (3.84,4.11] cL/g, and Q4 (4.11,11.74] cL/g. 21,006 hypertensive patients had a whole following time 97 (51, 151) months, 15,519 hypertensive patients were alive, and 5,487 were dead. The relationship between HSW and hypertension was analyzed. Results: Among Controls n=35,677 and Hypertensive patients n=24,190, the percentages of hypertension in quartiles of HSW (Q1, Q2, Q3, and Q4) were 28.59%, 33.35%, 39.37%, and 47.74%. Adjusted odds ratio (OR) in HSW was still significant, 1.23 (95% CI 1.11,1.36). Among dead (n=5,487) and alive hypertensive patients (n=15,519), the percentages of hypertensive mortality in quartiles of HSW were 17.66%, 20.46%, 20.78%, and 25.02%. The adjusted HSW hazard ratio (HR) was 1.91(95%CI 1.69,2.16). Processing Q1 as reference, the HR for Q4 was 2.35 (95% CI 2.06, 2.69). Males had a higher risk (HR: 1.53 95% CI 1.24,1.89) of poor prognosis than females (HR: 1.48 95% CI 1.17,1.87). Individuals <=60 years old (HR: 2.25 95% CI 1.78,2.85) had a higher risk of poor prognosis than those >60. Hypertensive patients with HSW > 3.89 cL/g had a poor prognosis than HSW <= 3.89 cL/g. Conclusions: HSW is an innovative independent risk factor for hypertensive prevalence and long-term prognosis.

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Section: Introductionmentioning

confidence: 99%

Hemoglobin specific volume width promotes the prevalence and poor long-term prognosis of American adult hypertensive patients: the NHANES 1999-2020

Zhang,

Chang,

Wang

et al. 2023

Preprint

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“…Red blood cells (RBC) are often used to investigate membrane lipid composition [7] and membrane protein function [8]. Compared to other tissues, such as adipose tissue, RBCs membranes are easily available and reflect long-term FA intake [9].…”

Section: Introductionmentioning

confidence: 99%

Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows

Revskij

Haubold

Viergutz

et al. 2019

IJMS

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Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed–safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC.

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“…RBC life span in the circulation is influenced by several complex factors (18 -20). Characteristic changes in the activity of ion transporters and cation content are related to RBC aging (21,22). Lack of several structural membrane skeleton proteins in RBCs reduces life span and induces anemia (23,24).…”

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confidence: 99%

Loss of the clock protein PER2 shortens the erythrocyte life span in mice

Sun

Zhao

Yang

et al. 2017

Journal of Biological Chemistry

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Cell proliferation and release from the bone marrow have been demonstrated to be controlled by circadian rhythms in both humans and mice. However, it is unclear whether local circadian clocks in the bone marrow influence physiological functions and life span of erythrocytes. Here, we report that loss of the clock gene significantly decreased erythrocyte life span. Mice deficient in were more susceptible to acute stresses in the erythrocytes, becoming severely anemic upon phenylhydrazine, osmotic, and HO challenges. H NMR-based metabolomics analysis revealed that the depletion causes significant changes in metabolic profiles of erythrocytes, including increased lactate and decreased ATP levels compared with wild-type mice. The lower ATP levels were associated with hyperfunction of Na/K-ATPase activity in -null erythrocytes, and inhibition of Na/K-ATPase activity by ouabain efficiently rescued ATP levels. -null mice displayed increased levels of Na/K-ATPase α1 (ATP1A1) in the erythrocyte membrane, and transfection of cDNA into the erythroleukemic cell line TF-1 inhibited expression. Furthermore, we observed that PER2 regulates transcription through interacting with trans-acting transcription factor 1 (SP1). Our findings reveal that function in the bone marrow is required for the regulation of life span in circulating erythrocytes.

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The Influence of Erythrocyte Maturity on Ion Transport and Membrane Lipid Composition in the Rat

Cited by 4 publications

References 27 publications

Hemoglobin specific volume width promotes the prevalence and poor long-term prognosis of American adult hypertensive patients: the NHANES 1999-2020

Hemoglobin specific volume width promotes the prevalence and poor long-term prognosis of American adult hypertensive patients: the NHANES 1999-2020

Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows

Loss of the clock protein PER2 shortens the erythrocyte life span in mice

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