Relationships between growth, cyclic amp and tylosin production in two mutants of Streptomyces fradiae

Colombo, Arnaldo Lopes; Crespi‐Perellino, N.; Micalizio, S.

doi:10.1007/bf00134671

Cited by 8 publications

(3 citation statements)

References 14 publications

(11 reference statements)

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“…In E. coli, cAMP is involved in carbon catabolite repression. Although cAMP occurs in Streptomyces species (Gersch, 1979;Colombo et al, 1982;Ensign, 1982;Surowitz & Pfister, 1985), it is uncertain whether it has an essential role in catabolite repression. In contrast with such negative regulations, compounds which 'positively' function have been reported recently.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of the Stringent Response in Streptomyces sp. and its Significance for the Initiation of Morphological and Physiological Differentiation

Ochi

1986

Microbiology

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Streptomyces sp. MA406-A-1 produced formycin (a nucleoside antibiotic) in parallel with cell growth in a synthetic medium. When the synthetic medium was supplemented with 1 % (w/v) Casamino acids, however, formycin was produced only after the end of exponential growth. The intracellular ppGpp pool increased gradually towards the end of exponential growth and was maximal at the beginning of formycin production. After shift down from Casamino acids medium to synthetic medium, the ppGpp pool increased immediately, while the GTP pool decreased ; under such conditions, the ability to produce formycin increased eightfold. Relaxed (re0 mutants, the first isolated for a Streptomyces species, were found at high incidence (10%) among spontaneous thiopeptin-resistant isolates and had severely reduced abilities to accumulate ppGpp. These re1 mutants also failed to produce formycin under the usual culture conditions and exhibited numerous pleiotropic effects such as an inability to produce melanin and an extended delay of aerial mycelium formation. Thus Streptomyces sp. exhibited a typical stringent response, and the response initiated (or was needed for) the induction of secondary metabolism. The response may have also participated in the initiation of aerial mycelium formation by decreasing the intracellular GTP pool.

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Section: Discussionmentioning

confidence: 99%

Occurrence of the Stringent Response in Streptomyces sp. and its Significance for the Initiation of Morphological and Physiological Differentiation

Ochi

1986

Microbiology

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“…l3 Also, the levels of cyclic AMP drop just before the onset of biosynthesis of the antibiotics streptomycin in S. griseus3' and turimycin in S. hygroscopicus. ' 6 The role of cyclic AMP seems to be more involved than what is implied above: Gersch and co-workers report that phosphate repression of turimycin synthesis is abolished by exogenously added cyclic AMP. l 3 With regard to tylosin biosynthesis, higher productivity tended to correlate with higher intracellular levels of cyclic AMP.…”

Section: Introductionmentioning

confidence: 99%

“…l 3 With regard to tylosin biosynthesis, higher productivity tended to correlate with higher intracellular levels of cyclic AMP. 6 Martin and Demain suggest that high cyclic AMP levels probably do not relieve repression of enzymes, as in enteric bacteria, but instead turn on antibiotic synthetases-this aspect being more closely related to phosphate regulation of secondary metab~lisrn.~~ Such a widely variable response to cyclic AMP is indicative of the possibility that the effects could be dependent on the nutritional status of the culture.…”

Section: Introductionmentioning

confidence: 99%

Cyclic AMP regulation of tylosin biosynthesis and secondary metabolism in Streptomyces fradiae

Tata

Menawat

1994

Biotech & Bioengineering

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Adenosine 3':5' cyclic monophosphate seems to regulate antibiotic biosynthesis and secondary metabolism in tylosin-producing cultures of Streptomyces fradiae C373.1. A dose-dependent response is observed by exogenous additions of dibutyryl cyclic AMP (cAMP), and is related to the nutritional status of the culture. Addition of cAMP to cultures growing in nutritionally lean media caused higher cumulative antibiotic tigers and some cellular differentiation compared with the control. In nutritionally rich media, a qualitatively different behavior resulted: an almost instantaneous shift toward secondary metabolism occurred. The response is characterized by extensive cellular differentiation with little growth and only a trace of antibiotic production. The possible role of cyclic AMP n the regulation of tylosin biosynthesis and secondary metabolism and its relation to specific nutrient limitations in synthetic, defined media in Streptomyces fradiae is discussed.

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Biochemical pathways and mechanisms nitrogen, amino acid, and carbon metabolism

Hütter

Niederberger

1983

Biotechnology Advances

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Relationships between growth, cyclic amp and tylosin production in two mutants of Streptomyces fradiae

Cited by 8 publications

References 14 publications

Occurrence of the Stringent Response in Streptomyces sp. and its Significance for the Initiation of Morphological and Physiological Differentiation

Occurrence of the Stringent Response in Streptomyces sp. and its Significance for the Initiation of Morphological and Physiological Differentiation

Cyclic AMP regulation of tylosin biosynthesis and secondary metabolism in Streptomyces fradiae

Biochemical pathways and mechanisms nitrogen, amino acid, and carbon metabolism

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