Relationships between antral follicle count, blood serum concentration  of anti-Müllerian hormone and fertility  in mares

Traversari, Julia; Aepli, H; Knutti, B.; Lüttgenau, Johannes; Bruckmaier, Rupert M.; Bollwein, Heinrich

doi:10.17236/sat00225

Cited by 7 publications

(12 citation statements)

References 59 publications

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“…A large variation in circulating AMH levels was observed between mares, ranging from 0.6 to 4.1 µg/L. This result is in agreement with previous studies where serum AMH concentrations ranged from 0.22 ng/mL to 2.94 ng/mL in normal cycling mares [35], from 0.07 to 3.56 ng/mL in pre-ovulatory mares [46], and from 0.26 ng/mL to 15.47 ng/mL in mares irrespective of the reproductive state [42]. Circulating AMH levels have been suggested to be related to the population of follicles measuring 6 to 20 mm [26].…”

Section: Discussionsupporting

confidence: 92%

“…However, it is worth mentioning that Traversari et al [46] did not find such a relation and that Uliani et al [42] observed a decrease in AMH concentrations only in mares older than 20 years. In agreement with previous observations, the correlation between the ovarian reserve and the amount of AMH in blood was shown to be stronger in older mares [26,46]. AMH levels were also positively correlated with the number of oocytes recovered in young and middle-aged mares.…”

Section: Discussionmentioning

confidence: 88%

“…Previous studies observed that AMH concentrations are significantly lower in older mares (16-27 years old), corresponding to a decline in the ovarian follicle pool [26,41]. However, it is worth mentioning that Traversari et al [46] did not find such a relation and that Uliani et al [42] observed a decrease in AMH concentrations only in mares older than 20 years. In agreement with previous observations, the correlation between the ovarian reserve and the amount of AMH in blood was shown to be stronger in older mares [26,46].…”

Section: Discussionmentioning

confidence: 88%

“…The relationship between AMH and fertility has been investigated previously and results seem to be controversial. While thoroughbred mares with lower AMH values appeared to have a lower pregnancy rate after mating [41], Traversari et al [46] observed no relationship between AMH concentration and pregnancy outcomes in warmblood mares. The current study indicates that the number of blastocysts produced per OPU session was significantly lower in mares whose AMH levels were below 1.5 µg/L.…”

Section: Discussionmentioning

confidence: 95%

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Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Papas

Govaere

Peere

et al. 2021

Animals

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Anti-Müllerian hormone (AMH) reflects the population of growing follicles and has been related to mammalian fertility. In the horse, clinical application of ovum pick-up and intracytoplasmic sperm injection (OPU-ICSI) is increasing, but results depend largely on the individuality of the mare. The aim of this study was to assess AMH as a predictor for the OPU-ICSI outcome in horses. Therefore, 103 mares with a total follicle count above 10 were included in a commercial OPU-ICSI session and serum AMH was determined using ELISA. Overall, the AMH level was significantly correlated with the number of aspirated follicles and the number of recovered oocytes (p < 0.001). Mares with a high AMH level (≥2.5 µg/L) yielded significantly greater numbers of follicles (22.9 ± 1.2), oocytes (13.5 ± 0.8), and blastocysts (2.1 ± 0.4) per OPU-ICSI session compared to mares with medium (1.5–2.5 µg/L) or low AMH levels (<1.5 µg/L), but no significant differences in blastocyst rates were observed. Yet, AMH levels were variable and 58% of the mares with low AMH also produced an embryo. In conclusion, measurement of serum AMH can be used to identify mares with higher chances of producing multiple in vitro embryos, but not as an independent predictor of successful OPU-ICSI in horses.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 95%

See 2 more Smart Citations

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Papas

Govaere

Peere

et al. 2021

Animals

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“…It has been reported that the antral follicle count (AFC), which is the amount of ovarian antral follicles, strongly correlates with the serum AMH levels and the number of primordial follicles. The AFC serves as an important marker of ovarian reserve ( Sakaguchi et al, 2019 ; Traversari et al, 2019 ). Therefore, we carried out morphometric analysis specific for distinct types of follicular populations.…”

Section: Discussionmentioning

confidence: 99%

Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress

Chen

Fan

et al. 2022

Front. Pharmacol.

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Premature ovarian insufficiency (POI) can result in lower fertility and shorten the female reproductive span. Bushen-Culuan Decoction (BCD) is a traditional Chinese medication utilized for treating POI for many years. We previously observed that BCD protects against further deterioration of the ovarian reserve of POI patients, however, the underlying mechanism has not been well studied. Our investigation seeks to evaluate the effect of BCD on POI induced by Tripterygium wilfordii polyglycosidium (TWP) and the likely mechanistic pathways, which we hypothesize may involve the Nrf2/ARE pathway. The body weights, estrous cycle, serum hormone levels, histological follicular analysis and quantification, levels of oxidative stress biomarkers in the ovarian tissue of POI mice models were evaluated. Western blotting and RT-PCR enabled quantification of the components of the Nrf2/ARE pathway. Our results showed that BCD restored hormonal profiles and estrous cycles of POI mice similar to those observed in healthy controls. BCD reduced the numbers of atretic follicles while increasing the number of primordial follicles. BCD facilitated lower 8-OHdG and MDA levels while increasing levels of key antioxidant enzymes including GSH-Px, CAT, and SOD. Furthermore, TWP increased Bach 1, Nrf2, and Keap 1 expressions at the translational level, while decreased that of HO-1. BCD treatment also promoted nuclear translocation rates of Bach 1 and Nrf2, suppressed Keap 1 protein expression, as well as raised HO-1 protein expression. Taken together, BCD likely augments ovarian reserve by activating the Nrf2/ARE signaling pathway, which stimulated higher levels of antioxidants and suppressed oxidative stress. BCD may be an important therapeutic compound in POI.

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Assessment and quantification of ovarian reserve on the basis of machine learning models

Ding

Ren²,

Han³

et al. 2023

Front. Endocrinol.

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BackgroundEarly detection of ovarian aging is of huge importance, although no ideal marker or acknowledged evaluation system exists. The purpose of this study was to develop a better prediction model to assess and quantify ovarian reserve using machine learning methods.MethodsThis is a multicenter, nationwide population-based study including a total of 1,020 healthy women. For these healthy women, their ovarian reserve was quantified in the form of ovarian age, which was assumed equal to their chronological age, and least absolute shrinkage and selection operator (LASSO) regression was used to select features to construct models. Seven machine learning methods, namely artificial neural network (ANN), support vector machine (SVM), generalized linear model (GLM), K-nearest neighbors regression (KNN), gradient boosting decision tree (GBDT), extreme gradient boosting (XGBoost), and light gradient boosting machine (LightGBM) were applied to construct prediction models separately. Pearson’s correlation coefficient (PCC), mean absolute error (MAE), and mean squared error (MSE) were used to compare the efficiency and stability of these models.ResultsAnti-Müllerian hormone (AMH) and antral follicle count (AFC) were detected to have the highest absolute PCC values of 0.45 and 0.43 with age and held similar age distribution curves. The LightGBM model was thought to be the most suitable model for ovarian age after ranking analysis, combining PCC, MAE, and MSE values. The LightGBM model obtained PCC values of 0.82, 0.56, and 0.70 for the training set, the test set, and the entire dataset, respectively. The LightGBM method still held the lowest MAE and cross-validated MSE values. Further, in two different age groups (20–35 and >35 years), the LightGBM model also obtained the lowest MAE value of 2.88 for women between the ages of 20 and 35 years and the second lowest MAE value of 5.12 for women over the age of 35 years.ConclusionMachine learning methods combining multi-features were reliable in assessing and quantifying ovarian reserve, and the LightGBM method turned out to be the approach with the best result, especially in the child-bearing age group of 20 to 35 years.

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Relationships between antral follicle count, blood serum concentration of anti-Müllerian hormone and fertility in mares

Cited by 7 publications

References 59 publications

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress

Assessment and quantification of ovarian reserve on the basis of machine learning models

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