Relationships between Antibodies and Experimental Allergic Encephalomyelitis

Relationships between Antibodies and Experimental Allergic Encephalomyelitis

“…4 Delayed hypersensitivity as measured by skin test with homologous BP [45]. 5 Cytophilic antibody; number of RBC adherent to 100 macrophages [22]. 6 Hemagglutination; median titer [24].…”

“…The following techniques were investigated for their usefulness in demonstrating anti bodies to BP in guinea pigs: passive cutaneous anaphylaxis or PCA [20]; hemagglutination or HA [24] with formalinized sheep red blood cells coupled to BP by bis-diazotized benzi dine; radioimmunoassays (RIA) by coprecipitation with anti-globulin antisera [32], by radioimmunodifTusion (RID), by radioimmunoelectrophoresis (RIE) and by Scphadex G-200 thin layer chromatographic (TLC) separation using 126I-labelcd BP; immunodiffu sion (ID) or immunoelectrophoresis (IE) in agarose [24] ; and cytophilic antibody (CA) assay [22] with fresh sheep red blood cells coupled to BP by bis-diazotized benzidine. In addition, delayed-type skin hypersensitivity (DH) to homologous (guinea pig) BP was studied [45].…”

“…From these results it is clear that encephalitogenic challenge (sensitization to BP with mycobacteria in the adjuvant) rather selectively evokes delayed hypersensitivity to BP. Cytophilic antibody is also evoked but occurs in only small amounts with acute EAE and not before [22]. Low titers of hemagglutinating antibodies can also be detected in acute EAE and these are sensitive to 2-mercaptoethanol [24].…”

“…Radioimmunoassay of antibody to BP [27]. Low titers of cytophilic [22] and hemagglutinating [24] antibodies to BP. Demyelinating [12] and synapse-blocking [13,19], complement-fixing antibodies to unidentified antigens of whole CNS.…”

“…The specific antigens evoking demyelinating [12] and synapse-blocking [13,19] antibodies remain to be defined, but the development of these complement-fixing antibodies in acute EAE evoked by whole CNS suggests parallels with the development of cytophilic antibody [22] to enccphalitogenic BP and of complement fixing antibody to nonencephalitogenic cerebroside (lipid) hapten [35][36][37][38]. Although this last has been related to resistance to EAE [36,37], such resistance of rats following recovery from acute EAE may be different from the resistance (protection) of guinea pigs and rabbits following immunization with BP in incomplete adjuvants [6,24,47].…”