Relationships among DNA adducts, micronuclei, and fitness parameters in Xenopus laevis exposed to benzo[a]pyrene

Sadinski, Walter J.; Lévay, György; Wilson, Michaile C.; Hoffman, Jennifer R.; Bodell, William J.; Anderson, Susan

doi:10.1016/0166-445x(94)00092-5

Cited by 19 publications

(8 citation statements)

References 53 publications

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“…It is also important to note from this series of experiments that RAPD profiling can detect DNA changes earlier than other changes in conventional toxicity assays measuring fitness parameters. After exposing larval Xenopus laevis to B a P, Sadinski et al [7] suggested that DNA adducts and micronuclei were sensitive measures of sublethal DNA damage as well as possible short—term indicators of indirect effects on fitness parameters. The present study, using a novel and simple approach, also supports this notion in a clonal freshwater invertebrate species.…”

Section: Discussionmentioning

confidence: 99%

Qualitative assessment of genotoxicity using random amplified polymorphic DNA: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene

Atienzar

Conradi

Evenden

et al. 1999

Enviro Toxic and Chemistry

194

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A method of DNA profiling using the random amplified polymorphic DNA (RAPD) was used to assess toxicant-induced DNA effects in laboratory populations of Daphnia magna exposed to varying concentrations of the genotoxic hydrocarbon benzo[a]pyrene. These effects, represented by changes in the RAPD profiles, were compared with a number of key ecological fitness parameters (age-specific survival, age-specific fecundity, net reproductive rate, and intrinsic rate of population increase). Not only was the RAPD profiling method shown to be a rapid and reproducible assay of toxicant-induced DNA effects, but the qualitative measure of genomic template stability compared favorably with the traditional indices of fitness. The RAPD profiles, however, exhibited higher sensitivity in detecting toxic effects. The significance of these findings for future ecotoxicological studies is discussed.

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Section: Discussionmentioning

confidence: 99%

Qualitative assessment of genotoxicity using random amplified polymorphic DNA: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene

Atienzar

Conradi

Evenden

et al. 1999

Enviro Toxic and Chemistry

194

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show abstract

“…In contrast to several physiologic endpoints, genotoxic responses may reflect ecotoxicological effects of greater importance, because they often have been more convincingly correlated with impairment of development, growth, and reproduction [4][5][6][7][8][9][10][11][12]. In addition, genotoxic endpoints tend to reflect exposure to a broad range of chemical stressors and may be more appropriate markers for initial screening of complex ag- otoxicity biomarker that responds to a wide spectrum of chemicals.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity in native fish associated with agricultural runoff events

Whitehead

Kuivila

Orlando

et al. 2004

Enviro Toxic and Chemistry

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The primary objective of the present study was to test whether agricultural chemical runoff was associated with in-stream genotoxicity in native fish. Using Sacramento sucker (Catostomus occidentalis), we combined field-caging experiments in an agriculturally dominated watershed with controlled laboratory exposures to field-collected water samples, and we coupled genotoxicity biomarker measurements in fish with bacterial mutagenicity analysis of water samples. We selected DNA strand breakage as a genotoxicity biomarker and Ames Salmonella mutagenicity tests as a second, supporting indicator of genotoxicity. Data from experiments conducted during rainfall runoff events following winter application of pesticides in 2000 and 2001 indicated that DNA strand breaks were significantly elevated in fish exposed to San Joaquin River (CA, USA) water (38.8, 28.4, and 53.6% DNA strand breakage in year 2000 field, year 2000 lab, and year 2001 field exposures, respectively) compared with a nearby reference site (15.4, 8.7, and 12.6% DNA strand breakage in year 2000 field, year 2000 lab, and year 2001 field exposures, respectively). Time-course measurements in field experiments supported a linkage between induction of DNA strand breakage and the timing of agricultural runoff. San Joaquin River water also caused significant reversion mutation in two Ames Salmonella tester strains. Salmonella mutagenicity corroborated in-stream effects, further strengthening a causal relationship between runoff events and genotoxicity. Potentially responsible agents are discussed in the context of timing of runoff events in the field, concordance between laboratory and field exposures, pesticide application patterns in the drainage, and analytical chemistry data.

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“…Coal tar pavement sealers effect amphibian growth 245 Sadinski et al (1995) found that when exposed to 248 nM benzo(a)pyrene Xenopus laevis tadpoles were smaller and took longer to metamorphose than the controls. The longer development time, in turn, predisposed those individuals to greater frequency of DNA adducts and red blood cells with micronuclei.…”

Section: Control N=10mentioning

confidence: 99%

“…Benzo(a)pyrene, a frequently encountered PAH, was lethal to newt larvae at low levels (50 ppb) (Fernandez and Lharidon, 1994). Sadinski et al (1995) showed that Xenopus laevis had slower development and growth after exposure to 249 nM benzo(a)pyrene, a sublethal concentration equivalent to approximately 60 ppb. Hatch and Burton (1998) compared the sensitivity of three species of amphibians and found that X. laevis was more sensitive than either Ambystoma maculatum or Rana pipiens to a combined exposure of fluoranthene and sunlight.…”

Section: Introductionmentioning

confidence: 98%

The Effects of Coal Tar Based Pavement Sealer on Amphibian Development and Metamorphosis

2006

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Coal tar based pavement sealers are applied regularly to parking lots and contain significant levels of polycyclic aromatic hydrocarbons (PAHs). Recently a connection between elevated levels of PAHs in streams and storm water runoff from parking lots has been identified. We tested the hypothesis that coal tar based pavement sealers could alter the survival, growth, and development of amphibians using a model species, Xenopus laevis. Ten fertilized individuals were placed singly into containers containing one of four treatment groups: control, low, medium, and high (respective nominal concentrations 0, 3, 30, and 300 ppm TPAH). All of the individuals in the high exposure group died by the sixth day of exposure. By day 14 there were significant patterns of stunted growth (p<0.0001) and slower development (p=0.006) in the medium and high exposure groups relative to the control and low treatment groups. When the experiment ended on day 52 the control and low-dose individuals had achieved more advanced developmental stages than the medium group (p=0.0007). These data indicate that these commonly used coal tar based pavement sealers may potentially affect the amphibian taxa living in areas that receive storm water runoff.

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Relationships among DNA adducts, micronuclei, and fitness parameters in Xenopus laevis exposed to benzo[a]pyrene

Cited by 19 publications

References 53 publications

Qualitative assessment of genotoxicity using random amplified polymorphic DNA: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene

Qualitative assessment of genotoxicity using random amplified polymorphic DNA: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene

Genotoxicity in native fish associated with agricultural runoff events

The Effects of Coal Tar Based Pavement Sealer on Amphibian Development and Metamorphosis

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