Qualitative assessment of genotoxicity using random amplified polymorphic DNA: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene

Abstract

A method of DNA profiling using the random amplified polymorphic DNA (RAPD) was used to assess toxicant-induced DNA effects in laboratory populations of Daphnia magna exposed to varying concentrations of the genotoxic hydrocarbon benzo[a]pyrene. These effects, represented by changes in the RAPD profiles, were compared with a number of key ecological fitness parameters (age-specific survival, age-specific fecundity, net reproductive rate, and intrinsic rate of population increase). Not only was the RAPD profiling method shown to be a rapid and reproducible assay of toxicant-induced DNA effects, but the qualitative measure of genomic template stability compared favorably with the traditional indices of fitness. The RAPD profiles, however, exhibited higher sensitivity in detecting toxic effects. The significance of these findings for future ecotoxicological studies is discussed.

Evaluation of genotoxicity of Pseudevernia furfuracea (L.) Zopf by RAPD analysis

“…GTS implies a qualitative measure reflecting changes in RAPD profiles. GTS related to the level of DNA damage, the efficiency of DNA repair and replication (Atienzar et al, 1999) could explain the appearance and disappearance of bands. The lowest values were obtained in samples no.…”

“…Polymorphism observed in RAPD profiles included the disappearance of a normal band and appearance of a normal band in comparison to RAPD profiles in the control (Atienzar et al, 1999;Liu et al, 2005) and the total number of band changes is given in Table 3.…”

Flavonoid constituents of Dobera glabra leaves: amelioration impact against CCl 4 -induced changes in the genetic materials in male rats

“…If left un-repaired, oxidative DNA damage can lead to detrimental biological consequences in organisms, including mutations and transformation of cells to malignant cells and cell death. Previous studies showed that changes in genomic DNA could reflect DNA alterations from single base changes (point mutations) to complex chromosomal rearrangements (Atienzar et al 1999(Atienzar et al , 2002.…”

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

“…Being a way of qualitative measurement of genotoxic effects, GTS is related to the level of DNA damage and also efficacy of DNA repair and replication (Atienzar et al 1999;Liu et al 2005;Cenkci et al 2010;Erturk et al 2012). GTS was used for comparing the changes in RAPD profiles, where generally in this study, GTS values tended to decrease with increasing concentrations of metal treatments.…”