Relationship of MTT reduction to stimulants of muscle metabolism

Newman, John; DiMaria, Carla A; Rattigan, Stephen; Steen, John; Miller, Kelly A.; Eldershaw, T. P. D.; Clark, Michael G.

doi:10.1016/s0009-2797(00)00192-7

Cited by 7 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of this, Zhang and Cox (1996) consider that the MTT assay may not be accurate under certain conditions. Various enzymes that may be found in serum, such as glutathione S-transferase, as well as other energy sources required for Na + pumping may also affect MTT reduction (York et al 1998;Newman et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells

et al. 2006

View full text Add to dashboard Cite

The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay is widely accepted as a simple and reproducible method for determining cell proliferation or cytotoxicity in vitro. In this study, we show that the flavonoids quercetin, rutin and luteolin but not apigenin can reduce MTT in the absence of live cells in the following order: quercetin >> rutin > luteolin > apigenin. Moreover, this reduction can be influenced by medium type and serum. The final concentrations of the flavonoids used were 200, 100, 50, 25 and 12.5 lg/mL. MTT reduction in Dulbecco's Modified Eagle's Medium (DMEM) is statistically higher than those in RPMI 1640 and F12 media, which are generally similar. Particularly for luteolin, MTT reduction is considerably higher with serum than without serum. In the case of quercetin at 50 lg/mL, a serum concentration of even only 0.01% is sufficient to significantly enhance MTT reduction versus that at 0% (P < 0.05). Serum at concentrations ranging from 0% to 5% also dosedependently affects the pattern of formazan crystal formation. In the presence of 0.156-5% serum, the formazan crystals gradually change from being small, numerous and scattered to being large, few and clumpy. The authors hypothesize that flavonoid structure, nutrient concentration in the culture medium as well as serum components directly affect MTT reduction by flavonoids in the absence of cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Membrane stabilizing doses of propranolol (inhibits Na + channels) or muscle contractions reversed the inhibitory effects on formazan production and metabolism changes. Plasma membrane polarization changes can affect charge gradient for MTT entry (Newman et al, 2000) Amyloid β peptide (Aβ)…”

Section: Angiotensinmentioning

confidence: 99%

Pitfalls of the MTT assay: Direct and off-target effects of inhibitors can result in over/underestimation of cell viability

Stepanenko

Dmitrenko

2015

Gene

241

158

View full text Add to dashboard Cite

“…The water-soluble PARP inhibitor PJ34 salvaged skeletal muscle viability following reperfusion. Tissue viability in this article and other stud-ies 27,28 of isolated cells and whole skeletal muscle tissues 29 was measured using a standard reproducible biochemical assay of mitochondrial activity. Mitochondrial activity was estimated by the reduction of a tetrazolium salt to water-insoluble colored formazan crystals by electron carriers and oxidative enzymes in the mitochondria of viable tissue.…”

Section: Commentmentioning

confidence: 99%

Polyadenosine Diphosphate–Ribose Polymerase Inhibition Modulates Skeletal Muscle Injury Following Ischemia Reperfusion

et al. 2005

View full text Add to dashboard Cite

show abstract

Relationship of MTT reduction to stimulants of muscle metabolism

Cited by 7 publications

References 18 publications

Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells

Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells

Pitfalls of the MTT assay: Direct and off-target effects of inhibitors can result in over/underestimation of cell viability

Polyadenosine Diphosphate–Ribose Polymerase Inhibition Modulates Skeletal Muscle Injury Following Ischemia Reperfusion

Contact Info

Product

Resources

About