Pitfalls of the MTT assay: Direct and off-target effects of inhibitors can result in over/underestimation of cell viability

Stepanenko, Aleksei A.; Dmitrenko, V. V.

doi:10.1016/j.gene.2015.08.009

Cited by 227 publications

(152 citation statements)

References 107 publications

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“…-4 -membrane, and are also partly associated with oxidative phosphorylation in mitochondria (Stepanenko and Dmitrenko 2015). Meanwhile a couple of assays using similar dyes such as MTS, WST or XTT are available as well.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

A guide to nanosafety testing: Considerations on cytotoxicity testing in different cell models

et al. 2018

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Section: Accepted Manuscriptmentioning

confidence: 99%

A guide to nanosafety testing: Considerations on cytotoxicity testing in different cell models

et al. 2018

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“…Tetrazolium-to-formazan reduction in colorimetric cell viability assays measures cell metabolic activity and often under-or overestimates the antiproliferative potential of drugs. 24 To account for this shortcoming and to determine the true number of viable, proliferating T cells, we used an assay based on direct cell counting. Purified, labeled CD8 + cells were treated with vehicle, rHSA, gold complexes, or the corresponding modified protein samples for 60 min, activated with the appropriate antibodies for 72 h, and analyzed for CFSE fluorescence (Figure 3).…”

mentioning

confidence: 99%

Human Serum Albumin-Delivered [Au(PEt₃)]⁺ Is a Potent Inhibitor of T Cell Proliferation

Dean

Yang

Liu

et al. 2017

ACS Med. Chem. Lett.

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“…The Trypan blue exclusion assay was also applied [23]. After time treatment, cells were stained with Trypan blue and counted in a hemocytometer.…”

Section: Methodsmentioning

confidence: 99%

Guanosine promotes cytotoxicity via adenosine receptors and induces apoptosis in temozolomide-treated A172 glioma cells

Oliveira

Dal-Cim

Lopes

et al. 2017

Purinergic Signalling

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Gliomas are a malignant tumor group whose patients have survival rates around 12 months. Among the treatments are the alkylating agents as temozolomide (TMZ), although gliomas have shown multiple resistance mechanisms for chemotherapy. Guanosine (GUO) is an endogenous nucleoside involved in extracellular signaling that presents neuroprotective effects and also shows the effect of inducing differentiation in cancer cells. The chemotherapy allied to adjuvant drugs are being suggested as a novel approach in gliomas treatment. In this way, this study evaluated whether GUO presented cytotoxic effects on human glioma cells as well as GUO effects in association with a classical chemotherapeutic compound, TMZ. Classical parameters of tumor aggressiveness, as alterations on cell viability, type of cell death, migration, and parameters of glutamatergic transmission, were evaluated. GUO (500 and 1000 μM) decreases the A172 glioma cell viability after 24, 48, or 72 h of treatment. TMZ alone or GUO plus TMZ also reduced glioma cell viability similarly. GUO combined with TMZ showed a potentiation effect of increasing apoptosis in A172 glioma cells, and a similar pattern was observed in reducing mitochondrial membrane potential. GUO per se did not elevate the acidic vesicular organelles occurrence, but TMZ or GUO plus TMZ increased this autophagy hallmark. GUO did not alter glutamate transport per se, but it prevented TMZ-induced glutamate release. GUO or TMZ did not alter glutamine synthetase activity. Pharmacological blockade of glutamate receptors did not change GUO effect on glioma viability. GUO cytotoxicity was partially prevented by adenosine receptor (A 1 R and A 2A R) ligands. These results point to a cytotoxic effect of GUO on A172 glioma cells and suggest an anticancer effect of GUO as a putative adjuvant treatment, whose mechanism needs to be unraveled.

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Pitfalls of the MTT assay: Direct and off-target effects of inhibitors can result in over/underestimation of cell viability

Cited by 227 publications

References 107 publications

A guide to nanosafety testing: Considerations on cytotoxicity testing in different cell models

A guide to nanosafety testing: Considerations on cytotoxicity testing in different cell models

Human Serum Albumin-Delivered [Au(PEt₃)]⁺ Is a Potent Inhibitor of T Cell Proliferation

Guanosine promotes cytotoxicity via adenosine receptors and induces apoptosis in temozolomide-treated A172 glioma cells

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Pitfalls of the MTT assay: Direct and off-target effects of inhibitors can result in over/underestimation of cell viability

Cited by 227 publications

References 107 publications

A guide to nanosafety testing: Considerations on cytotoxicity testing in different cell models

A guide to nanosafety testing: Considerations on cytotoxicity testing in different cell models

Human Serum Albumin-Delivered [Au(PEt3)]+ Is a Potent Inhibitor of T Cell Proliferation

Guanosine promotes cytotoxicity via adenosine receptors and induces apoptosis in temozolomide-treated A172 glioma cells

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Human Serum Albumin-Delivered [Au(PEt₃)]⁺ Is a Potent Inhibitor of T Cell Proliferation