Relationship of colonization and sporulation by VA mycorrhizal fungi to plant nutrient and carbohydrate contents*

Douds, David D.; Schenck, N. C.

doi:10.1111/j.1469-8137.1990.tb00547.x

Cited by 95 publications

(56 citation statements)

References 28 publications

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“…One of the factors contributing to this result was probably the low concentration of P in this nutrient solution (3 μM). High concentration of P can reduce hyphal growth (23), as well as colonization and sporulation of AMF (10), while the reduction can improve spore production (9,38). On the other side, high sporulation of G. etunicatum was obtained in substrate irrigated with S1B or with S3, what indicates possible plasticity of this isolate in relation to wide values of pH and P concentration in the nutrient solution.…”

Section: Discussionmentioning

confidence: 93%

Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Silva¹,

Yano‐Melo²,

Brandão³

et al. 2005

Braz. J. Microbiol.

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The production of inoculum is one of the hindrances in the large scale application of arbuscular mycorrhizal fungi (AMF). The objective of this work was to evaluate the effect of nutrient solutions with or without TrisHCl buffer, on sporulation of AMF. The experiment was carried out in a greenhouse, using a substrate with sand and vermiculite (1:1 v/v). Fifty spores of Gigaspora margarita, Scutellospora heterogama, and Glomus etunicatum were inoculated in Sorghum vulgare (sorghum) or Panicum miliaceum (fodder millet). The substrate received the following nutrient solutions: Hoagland with 3 μM P (S1); Long Ashton II with 15.9 μM P (S2) and Hoagland with 20 μM P (S3), with or without 50 mM of Tris-HCl buffer (pH 6.5); the control treatment, consisting of a soil + sand + vermiculite (2:1:1 v/v) substrate was irrigated with deionized water. Ten weeks after the beginning of the experiment sporulation did not differ in treatments with sorghum. Panicum miliaceum promoted higher sporulation of the AMF than sorghum, and differences among treatments with nutrient solutions were observed. Production of spores of G. margarita and S. heterogama increased significantly after addition of buffer in S1 and S2, while that of G. etunicatum was improved when the substrate was irrigated with S1 + buffer and S3 solutions. Solution S1 + buffer benefited sporulation of the three fungi. However, as observed, each AMF, host, and substrate system should be studied separately for establishment of the most favorable conditions for inoculum production.

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Section: Discussionmentioning

confidence: 93%

Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Silva¹,

Yano‐Melo²,

Brandão³

et al. 2005

Braz. J. Microbiol.

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“…Clapp et al (1995), using molecular techniques, demonstrated a positive relationship between the distribution of spores and root colonization in Acaulospora and Scutellospora species under field conditions. More detailed works, carried out under controlled conditions, suggest that the production of spores is related more strongly to the length of root colonization than to the percent root colonization (Douds & Schenck 1990, Gazey et al 1992. Other papers showed that plants with finer roots favoured the sporulation of AMF (Kormanick et al 1980), and yet others correlated the increased number of spores with the improved nutritional state of the plants (Louis & Lim 1987).…”

Section: Resultsmentioning

confidence: 99%

Effects of using different host plants on the detected biodiversity of arbuscular mycorrhizal fungi from an agroecosystem

Carrenho¹,

Trufem²,

Bononi³

2002

Rev. bras. Bot.

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(Effects of using different host plants on the detected biodiversity of arbuscular mycorrhizal fungi from an agroecosystem). The influence of peanut (Arachis hypogaea L.), sorghum (Sorghum bicolor (L.) Moench) and maize (Zea mays L.) on the development and diversity of arbuscular mycorrhizal fungi (AMF) from an agrosystem was investigated. Soil collected from an agricultural field where maize had been grown was inserted into sowing holes, under the seeds of peanut, sorghum and maize those were subsequently grown in sterilised quartz sand separately in plastic boxes for five months. After this period, collections of roots and rhizospheric soil were made to evaluate the percentages of root colonization (RC), number of spores (NS) and species of AMF. Peanut showed the highest average values for RC and NS: 24.5% and 547.8/100 g of soil, respectively. Maize had an average RC of 19.7% and 415.2 spores/100g soil. Sorghum showed the lowest values: 15.9% for average RC and 349.8 spores/100 g soil. A total of fourteen species of AMF were identified. Seven species were identified from peanut rhizospheres, Entrophospora colombiana being the most abundant and frequent. In sorghum rhizospheres, twelve species were found, Glomus geosporum was the dominant taxon in terms of number of spores and frequency. Ten species were detected in maize with Acaulospora longula being the most abundant and the most frequent. It was observed that peanut was the best plant for promoting the sporulation of AMF, while sorghum favoured the establishment of most AMF species, followed by maize. RESUMO (Efeitos do uso de diferentes plantas hospedeiras na biodiversidade detectada de fungos micorrízicos arbusculares de um agroecossistema). O objetivo deste trabalho foi estudar a influência de amendoim (Arachis hypogaea L.), sorgo (Sorghum bicolor (L.) Moench) e milho (Zea mays L.) sobre o desenvolvimento e diversidade de fungos micorrízicos arbusculares (FMA).Esporos de FMA, multiplicados anteriormente em milho, foram depositados sob sementes de amendoim, sorgo ou milho em caixas plásticas contendo areia de rio esterilizada. Cinco meses depois, amostras de raízes e solo rizosférico das três plantas hospedeiras foram coletadas para avaliar-se os parâmetros: colonização radical (CR), número de esporos (NE) e diversidade de espécies de FMA. Amendoim apresentou os valores mais altos de CR e NE: 24,5% e 547,8/100 g solo, respectivamente; milho teve valores menores para CR e NE: 19,7% e 415,2 esporos/100g solo, respectivamente; e sorgo apresentou em média 15,9% de CR e 349,8 esporos/100 g solo. Ao todo, foram isoladas e identificadas quatorze espécies de FMA. Sete foram verificadas em milho, sendo Entrophospora colombiana a mais abundante e freqüente. Em sorgo verificou-se 12 espécies, sendo Glomus geosporum o táxon dominante. Em milho foram isoladas dez espécies, sendo Acaulospora longula a mais abundante e freqüen-te. Pôde-se observar também que amendoim foi a planta que mais promoveu a esporulação dos FMA, enquanto sorgo favoreceu o estabelecimen...

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“…Because mycorrhizae help to increase uptake of minerals in plants, the levels of P and Zn in the plant may regulate root colonization and spore formation (Smith and Read, 1997). It has been shown that high levels of phosphorus in soil and plant are able to inhibit mycorrhiza formation (Douds and Schenck, 1990) and influence the diversity of AM fungi in field soils (Cuenca and Menses, 1996).…”

Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungi associated with shade trees and Coffea arabica L. in a coffee-based agroforestry system in Bonga, Southwestern Ethiopia

Sewnet

Tuju

2013

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(1) Haramaya University, Ethiopia (2) Addis Ababa University, EthiopiaIn a first step to understand the interactions between Coffea arabica L. trees and mycorrhizae in Ethiopia, an investigation of the current mycorrhizal colonization status of roots was undertaken. We sampled 14 shade tree species occurring in coffee populations in Bonga forest, Ethiopia. Scutellospora (Sp1 & Sp2) and Gigaspora (Sp1 & Sp2) were found under all 14 shade tree species, whereas Acaulospora (Sp1) occurred only in slightly acidic soils, within a pH range of 4.93-5.75. Generally, roots of the coffee trees were colonized by arbuscules to a greater degree than those of their shade trees, the arbuscular colonization percentage (AC%) of the former being higher than the latter (significant difference at 0.05 level). Though differences were not statistically significant, the overall hyphal colonization percentage (HC%) and mycorrhizal hyphal colonization percentage (MHC%) were shown to be slightly higher under coffee trees than under their shade trees. However, the differences were statistically significant at 0.05 level in the case of HC% values of coffee trees under Pouteria adolf-friederici and MHC% under Cordia africana. Spore density and all types of proportional root colonization parameters (HC%, MHC%, AC% and vesicular colonization percentage, VC%) for both coffee and shade trees were negatively and significantly correlated with organic soil carbon, total N, available P, EC and Zn. Correlation between arbuscular colonization for coffee (AC%) and organic carbon was not significantly positive at a 0.05 level. Incidence of specific spore morphotypes was also correlated with physical and chemical soil properties. Results indicate that AM fungi could potentially be important in aforestation and help to promote coffee production activities in Ethiopia providing an alternative to expensive chemical fertilizer use, and would offer management methods that take advantage of natural systems dynamics that could potentially preserve and enhance coffee production.

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Relationship of colonization and sporulation by VA mycorrhizal fungi to plant nutrient and carbohydrate contents*

Cited by 95 publications

References 28 publications

Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Effects of using different host plants on the detected biodiversity of arbuscular mycorrhizal fungi from an agroecosystem

Arbuscular mycorrhizal fungi associated with shade trees and Coffea arabica L. in a coffee-based agroforestry system in Bonga, Southwestern Ethiopia

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