Relationship of cell wall composition toin vitro cell wall digestibility of maize inbred line stems

Méchin, Valérie; Argillier, O.; Menanteau, Véronique; Barrière, Yves; Mila, Isabelle; Pollet, Brigitte; Lapierre, Catherine

doi:10.1002/(sici)1097-0010(200004)80:5<574::aid-jsfa575>3.0.co;2-r

Cited by 100 publications

(109 citation statements)

References 36 publications

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“…These traits typically exhibit a genotype by environment interaction effect and have complex patterns of inheritance indicative of control by many genes. QTLs affecting mixed-linked glucan content in oat (Avena sativa) and barley, fiber content in maize, and ratio of Ara to Xyl in wheat flour have been detected (Han et al, 1995;Lü bberstedt et al, 1997Lü bberstedt et al, , 1998Martinant et al, 1998;Kianian et al, 2000;Méchin et al, 2000). However, the specific genes underlying the QTLs identified in any of these studies, due to the complexity of the relevant plant genome, the lack of adequate genomic resources, or both.…”

supporting

confidence: 79%

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Quantitative Trait Loci and Comparative Genomics of Cereal Cell Wall Composition

et al. 2003

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Quantitative trait loci (QTLs) affecting sugar composition of the cell walls of maize (Zea mays) pericarp were mapped as an approach to the identification of genes involved in cereal wall biosynthesis. Mapping was performed using the IBM (B73 × Mo17) recombinant inbred line population. There were statistically significant differences between B73 and Mo17 in content of xylose (Xyl), arabinose (Ara), galactose (Gal), and glucose. Thirteen QTLs were found, affecting the content of Xyl (two QTLs), Ara (two QTLs), Gal (five QTLs), Glc (two QTLs), Ara + Gal (one QTL), and Xyl + Glc (one QTL). The chromosomal regions corresponding to two of these, affecting Ara + Gal and Ara on maize chromosome 3, could be aligned with a syntenic region on rice (Oryza sativa) chromosome 1, which has been completely sequenced and annotated. The contiguous P1-derived artificial chromosome rice clones covering the QTLs were predicted to encode 117 and 125 proteins, respectively. Two of these genes encode putative glycosyltransferases, displaying similarity to carbohydrate-active enzyme database family GT4 (galactosyltransferases) or to family GT64 (C-terminal domain of animal heparan synthases). The results illustrate the potential of using natural variation, emerging genomic resources, and homeology within the Poaceae to identify candidate genes involved in the essential process of cell wall biosynthesis.

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confidence: 79%

“…When cross-sections of intact seeds were observed by fluorescence microscopy, three types of cells were visible at the edge (Fig. 1, A and B;Kiesselbach, 1949). The pericarp is the outermost layer and is composed of approximately 10 thick-walled cells that fluoresce blue.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Trait Loci and Comparative Genomics of Cereal Cell Wall Composition

et al. 2003

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“…Most of the above studies used materials with different lignin contents and͞or compositions as a result of divergent selection (15), natural genetic variation (16), delignification (17), or maturity (18). Such studies are complicated by the many uncontrolled genetic and developmental variables potentially affecting digestibility (19).…”

mentioning

confidence: 99%

Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa ( Medicago sativa L.)

Reddy

Chen

Shadle

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

316

328

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Improving the digestibility of forages provides a means to enhance animal performance and protect the environment against excessive animal waste. Increased lignin content during maturity, and corresponding changes in lignin composition, correlate with decreased digestibility of forages. These relationships have yet to be investigated in isogenic systems. By targeting three specific cytochrome P450 enzymes of the lignin pathway for antisense downregulation, we generated transgenic alfalfa lines with a range of differences in lignin content and composition. There was a strong negative relationship between lignin content and rumen digestibility, but no relationship between lignin composition and digestibility, in these transgenic lines. Models for genetic manipulation of forage digestibility based on the changes in lignin composition that increase paper-pulping efficiency in trees are therefore invalid. Down-regulation of 4-coumarate 3-hydroxylase provided the largest improvements in digestibility yet seen in a forage crop.forage digestibility ͉ lignin modification ͉ metabolic engineering

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“…Conversely, this yield is decreased when the frequency of lignin units involved 12 in resistant interunit bonds, referred to as condensed linkages, is high. In agreement with previously published data (Méchin et al 2000), the lignin-derived monomers released from the lignins of the extractive-free maize stem were obtained with a yield close to 800 µmol per gram lignin (Table 4 The β-O-4 yield of LC1 accounted for 54% of the initial amount of the MS EFR, calculated using data in Table 1 and Table 4 These two alkali-soluble fractions were further characterized by analyzing the amount and linkage types with respect to p-coumaric and ferulic acids. (Table 5).…”

Section: Thioacidolysis Revealed Contrasting Lignin Structures Of Lc1mentioning

confidence: 99%

“…(Table 5). The content of ester-linked CA observed in the current study for MS EFR is in agreement with literature value for F2 maize line (Méchin et al, 2000). By contrast, the LC or R fractions recovered after alkaline hydrolysis were found to be enriched in FA and CA units that resist mild alkaline hydrolysis.…”

Section: Thioacidolysis Revealed Contrasting Lignin Structures Of Lc1mentioning

confidence: 99%

Isolation of structurally distinct lignin–carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment

Sipponen

Lapierre

Méchin

et al. 2013

Bioresource Technology

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Sequential fractionation of extractive-free maize stems was carried out using two mild alkaline extractions (0.5 M and 2 M NaOH, 20°C, 24 h) before and after endoglucanase treatment. This procedure provided two lignin-carbohydrate fractions (LC1 and LC2) recovered after each alkali treatment. LC1 and LC2 contained 39% and 8% of the total lignin amount, respectively. These two fractions contained structurally distinct lignin molecules. While the content of resistant interunit bonds in lignin was 77% in LC1, it was increased up to 98% in LC2. Not unexpectedly, both alkali-soluble fractions contained substantial amount of p-coumaric and ferulic acids ether-linked to lignins. These results outline heterogeneity of maize stem lignins related to fractionation of grass materials.

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Relationship of cell wall composition toin vitro cell wall digestibility of maize inbred line stems

Cited by 100 publications

References 36 publications

Quantitative Trait Loci and Comparative Genomics of Cereal Cell Wall Composition

Quantitative Trait Loci and Comparative Genomics of Cereal Cell Wall Composition

Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa ( Medicago sativa L.)

Isolation of structurally distinct lignin–carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment

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