Relationship of bacteriocin-like inhibitor production to the pigmentation and hemolytic activity of mutans streptococci

Crooks, Marguerite; James, S. M.; Tagg, John

doi:10.1016/s0176-6724(87)80197-9

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…Only strain UA96 was hemolytic on blood agar. These observations are consistent with our previous ¢ndings [12] of an apparent lack of direct correlation between either pigmentation or hemolytic activity and bacteriocin production in mutans streptococci. Mutacin N activity eluted from the C8 reversed phase column at approximately 41% AcN and from the C18 reversed phase column at approximately 50% AcN suggesting that it is relatively hydrophobic.…”

Section: Resultssupporting

confidence: 93%

Streptococcus mutans strain N produces a novel low molecular mass non-lantibiotic bacteriocin

Balakrishnan¹

2000

FEMS Microbiology Letters

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Streptococcus mutans strain N was shown to have bacteriocin production and immunity characteristics consistent with those of Group I mutacin-producing strains of S. mutans. The bacteriocin mutacin N was purified from agar cultures of S. mutans strain N using XAD and reversed phase chromatography. The molecular mass of mutacin N was 4806 Da and the entire 49 amino acid sequence was determined by N-terminal sequencing. Database searches indicate that mutacin N is a novel bacteriocin, but with some homology to the protein IIC domain of a hypothetical sugar-phosphotransferase enzyme from Acholeplasma florum. ß

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Section: Resultssupporting

confidence: 93%

Streptococcus mutans strain N produces a novel low molecular mass non-lantibiotic bacteriocin

Balakrishnan¹

2000

FEMS Microbiology Letters

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“…Crooks and coworkers [9] previously reported that strain D14 (group B) was haemolytic on human blood agar. Although not fully characterised, the association of bacteriocin production and haemolytic activity has been reported previously in some S. mutans [9] and S. salivarius [47]. The production of a protein that combines bacteriocin and haemolytic activity (bacteriocin/haemolysin) by E. faecalis strains is now well documented [48].…”

Section: Discussionmentioning

confidence: 99%

“…The haemolytic activity of both mutacin producers and non-producers was tested with double-layer agar plates consisting of a base layer of saline agar (NaCl 0.85% plus Davis agar 1.5%) and a top layer of Columbia agar base (CAB; Gibco BRL, Life Technologies, Paisley, Scotland) containing human blood 5% v/v [9]. Test colonies were inoculated into these plates with sterile tooth picks and incubated in an atmosphere of CO 2 5% in air at 378C for 48 h. Pigment production was assessed by visual examination of colonies for yellow pigmentation after anaerobic incubation for 2 days on TYCSB medium [30].…”

Section: Haemolytic Activity and Pigment Productionmentioning

confidence: 99%

“…The term BLIS has been adopted when referring to bacteriocin-like inhibitory effects before complete characterisation of the inhibitory molecule(s) [5]. The reported incidence of BLIS production among mutans streptococci has ranged from 11% to 100% [6][7][8][9][10][11][12][13][14], the differences in detection frequencies probably reflecting differences in the test conditions and in the BLIS indicator strains used.…”

Section: Introductionmentioning

confidence: 99%

“…A number of years ago a bacteriocin typing scheme first developed in this laboratory [26] was applied to the study of BLIS production among mutans streptococci [9]. It was found that 39% of 18 culture collection strains and 69% of 35 fresh isolates produced inhibitory activity with 7 and 13 different patterns of inhibitory activity (P-types), respectively, being distinguished against 9 standard indicator strains.…”

Section: Introductionmentioning

confidence: 99%

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Different bacteriocin activities of Streptococcus mutans reflect distinct phylogenetic lineages

Balakrishnan

Simmonds

Kilian

et al. 2002

Journal of Medical Microbiology

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Bacteriocins produced by mutans streptococci are known as mutacins. In this study 16 broadly active mutacin-producing Streptococcus mutans strains from New Zealand, North America and Europe were classified into four groups (A-D) on the basis of differences in their activity in deferred antagonism tests against either the homologous producer strain (to test for presence of self-immunity) or indicator strains Staphylococcus aureus 46 and Enterococcus faecium TE1. Two of the strains included in the study (UA140 and UA96) were representatives of the group I and II mutacin producer strains previously described by Caufield and co-workers. One of the New Zealand isolates of group A (S. mutans strain N) appeared to produce inhibitory activity similar to that of the group I prototype strain UA140. Four other New Zealand isolates of group B (S. mutans strains M19, M34, B34 and D14) had mutacin II-like activity. The group B mutacin producers differed from the group A mutacin producers in their additional activity against Staph. aureus 46. Seven S. mutans strains (M46, B46, B57, M12, M28, B28 and 13M) were distinguished from the group A and group B mutacin producers in that they inhibited E. faecium TE1. These were called group C mutacin producers. Strains H7 and H23 resembled the group C strains in their action on both indicator strains TE1 and 46. However, these two strains failed to exhibit immunity to their own inhibitory products in the deferred antagonism test and were separately classified as group D mutacin producers. Phylogenetic analysis of the strains by several genotypic and phenotypic characteristics revealed that the mutacin groups were associated with distinct evolutionary lineages of S. mutans.

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Streptococcus rattusstrain BHT produces both a class I two-component lantibiotic and a class II bacteriocin

Hyink

Balakrishnan

Tagg

2005

FEMS Microbiology Letters

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Streptococcus rattus strain BHT is a species representative and strong bacteriocin producer. Here we report that S. rattus BHT produces two quite different types of bacteriocin activity, named BHT-A and BHT-B. The two bacteriocins were purified and analysed for activity and by MALDI-TOF mass spectrophotometry. BHT-A was found to be a variant of the two-component lantibiotic, Smb. BHT-B is a non-modified 5195Da peptide with some similarity to the tryptophan-rich Staphylococcus aureus bacteriocin, aureocin A53. Six S. rattus and two S. mutans strains were found to contain both the BHT-A and BHT-B genetic loci.

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Relationship of bacteriocin-like inhibitor production to the pigmentation and hemolytic activity of mutans streptococci

Cited by 9 publications

References 19 publications

Streptococcus mutans strain N produces a novel low molecular mass non-lantibiotic bacteriocin

Streptococcus mutans strain N produces a novel low molecular mass non-lantibiotic bacteriocin

Different bacteriocin activities of Streptococcus mutans reflect distinct phylogenetic lineages

Streptococcus rattusstrain BHT produces both a class I two-component lantibiotic and a class II bacteriocin

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