Relationship between the subcellular distribution of delta-9-tetrahydrocannabinol and its metabolites in rat brain and the duration of the effect on motor activity

Metabolic mapping of the time-dependent effects of Δ 9 -tetrahydrocannabinol administration in the rat

“…Measures of core body temperature, spontaneous locomotor activity, catalepsy, as well as antinociception, have all been reported to be maximally altered within approximately 1 h after administration (Magour et al 1976;Carta et al 1999;Nava et al 2000;Freedland et al, unpublished data). The duration of these effects, however, can vary considerably.…”

“…Low doses of THC administered to rats, for example, have been shown to augment spontaneous locomotor behavior, whereas higher doses reduce locomotor activity (Sañudo-Peña et al 2000). In terms of duration of action, the behavioral effects of THC have been observed for as long as 4-8 h after drug administration, even though maximal effects occurred within the first hour (Magour et al 1976). Because of the long terminal half-life of THC, it is likely that subtle effects may persist even in the absence of most behavioral disruptions.…”

The Effect of Mercury Chloride and Methyl Mercury on Brain Microsomal Na+‐K+‐ATPase after Partial Delipidisation with Lubrol®

“…Food and water were provided ad libirum. Brain subcellular fractions were prepared as previously described (Magour et al 1976(Magour et al & 1984. Microsomal or synaptosomal pellets were either suspended in Tris/ HCl buffer, pH 7.4, for immediate use or frozen at -20" to be used during the subsequent two weeks.…”