Relationship between the Papain Sensitivity of Human γG Immunoglobulins and their Heavy Chain Subclass

Jefferis, Royston; Weston, P.D.; Stanworth, D.R.; Clamp, John R.

doi:10.1038/219646b0

Cited by 68 publications

(13 citation statements)

References 12 publications

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“…For example, IgGl and IgG3 are readily digested by papain, whereas IgG2 and IgG4 are more resistant [ 9 ] . With respect to complement fixation, IgG3 followed by IgCl are the most effective, IgG2 less so and lgG4 virtually inactive [ 10, 1 I].…”

Section: Discussionmentioning

confidence: 99%

The binding of human lgG subclasses to human monocytes

1972

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Section: Discussionmentioning

confidence: 99%

The binding of human lgG subclasses to human monocytes

1972

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“…The IgG2-rich fraction was eluted between pH 5.2 and pH 4.6, whereas the corresponding IgG1-rich fraction was eluted between pH 4.6 and pH 4.0. Contaminating IgG1 in the IgG2 fraction was removed by papain (Sigma, St Louis, MO, USA) digestion in the absence of any reducing agents (enzyme:substrate ¼ 1:100) for 16 h. The IgG2 is resistant to papain digestion at nonreducing conditions [20]. All fractions were concentrated with ultrafiltration (Ultrahülsen: Schleicher & Schuell, Dassel, Germany), dialysed against PBS pH 7.4 without a preservative, aliquoted and stored at ¹20ЊC.…”

Section: Methodsmentioning

confidence: 99%

Opsonophagocytic and Bactericidal Activity Mediated by Purified IgG Subclass Antibodies After Vaccination with the Norwegian Group B Meningococcal Vaccine

Aase

Ea²,

Michaelsen

1998

Scand J Immunol

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Aase A, Høiby EA, Michaelsen TE. Opsonophagocytic and Bactericidal Activity Mediated by Purified IgG Subclass Antibodies After Vaccination with the Norwegian Group B Meningococcal Vaccine. Scand J Immunol 1998;47:388-396 To study how the different immunoglobulin (Ig)G subclass antibodies may confer protection against systemic meningococcal disease, we isolated IgG1, IgG2 and IgG3 antibodies from plasma from vaccinees immunized with the Norwegian meningococcal outer membrane vesicle vaccine. Four IgG1, one IgG2 and four IgG3 preparations were purified. The IgG2 and IgG3 subclass preparations were free from contaminating subclasses, whereas the IgG1 preparations contained from 0 to 14% of IgG2 and/or IgG3. Immunoblotting against whole-cell meningococcal antigens showed broad specificities of the various preparations, both within and between subclasses. These subclass preparations were tested for opsonophagocytic and bactericidal activity. As targets we used two different variants of the meningococcal vaccine strain, with low (44/76-SL) and high (44/76-1) expression of the outer membrane protein Opc. Using polymorphonuclear leucocytes as effector cells in the presence of human complement, all three IgG subclass preparations revealed high, and similar, opsonophagocytic activities against 44/76-SL, whereas against 44/76-1 the IgG2 preparation showed a reduced activity and most IgG3 preparations were slightly more active than the IgG1 preparations. Regarding bactericidal activity, all the three subclasses were highly active against 44/76-SL. Against 44/ 76-1 the bactericidal activities were somewhat more varied: all IgG1 and three IgG3 preparations exhibited higher activities than against 44/76-SL. Due to the low concentration in the IgG2 preparations, only a weak activity was seen against 44/76-1. One IgG3 preparation that was highly opsonophagocytic revealed no bactericidal activity against either of the two bacterial variants examined. In conclusion, we have shown that the IgG subclass effector functions differ from person to person, but that antibodies of IgG1, IgG2 and IgG3 subclasses, judged by their behaviour in the functional tests, may all contribute to protection against meningococcal disease.

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“…The remainder are, in general, more susceptible to peptic digestion than either colostral or serum IgA. Precedent for varying susceptibility of immunoglobulins to enzymatic digestion can be found in the relationships between sensitivity to papain and the heavy-chain subclass of IgG (25). It should be noted that relative susceptibility of these proteins to peptic digestion, as measured by both the TCA and the Sephadex G200 methods, may reflect the position rather than the number of peptide bonds hydrolyzed (26).…”

Section: Discussionmentioning

confidence: 97%