Relationship between the matrix effect and the physicochemical properties of analytes in gas chromatography

Saka, Kanju; Kudo, Kazuhiko; Hayashida, Makiko; Kurisaki, Emiko; Niitsu, Hisae; Terada, Masaru; Yamaguchi, Koji; Yoshida, Ken

doi:10.1007/s00216-013-7433-9

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…Based on the results of the method validation, the deviation resulting from the analytical method was excluded, and the ME was considered to be the primary cause of the error in the GC/MS analysis. Although the ME is related to the GC/MS system, analyte nature, matrix type, and the concentration of the analyte, its underlying mechanisms are not completely understood 14,26 . In our study, the possible reasons were as follows.…”

Section: Resultsmentioning

confidence: 90%

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

Zhao

Zhang

Guo

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale The matrix effect is tricky in gas chromatography/mass spectrometry (GC/MS) analyses. Although several methods have been proposed to solve this problem, the results were unsatisfactory. Even fewer studies have assessed the performance of corrective methods. Hence, our study focused on assessing several common corrective methods, and then proposed a new strategy to correct for the matrix effect in GC/MS analyses. Methods In GC/MS analyses, the internal standard method (ISM) was employed to overcome the matrix effect during the detection of pyruvic acid (PA) in serum samples from a healthy adult female. The accuracy of the ISM was evaluated by comparing it with the standard addition method (SAM). To employ the ISM‐SAM strategy, correction factors (CFs) were established by combining the ISM and the SAM based on different groups. The CFs were used to normalize data onto the results of subsequent analyses. Results When using the ISM to detect levels of PA, a serious bias is observed, thereby affecting the conclusions reached. In contrast, more reliable data can be obtained after normalizing results by undertaking the ISM‐SAM strategy. The feasibility of this strategy was verified by comparing it with the results of the SAM alone. The ISM‐SAM strategy was successfully applied to quantify the PA levels in healthy people and nephrotic syndrome patients. Conclusions Our results indicated that a false outcome was presented when only the ISM was used to adjust the data, and important information would be missed if the correction strategy was not carried out. Therefore, ISM‐SAM, as an available correction method, should be adapted to improve the reliability of research results.

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Section: Resultsmentioning

confidence: 90%

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

Zhao

Zhang

Guo

et al. 2021

Rapid Comm Mass Spectrometry

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“…Saka et al. developed a predictive model for the matrix effect in GC analysis using quantitative structure–property relationships . They concluded that increases in molecular polarity (particularly H‐bond donors) and the volume of the analyte increase the matrix effect, while hydrophobicity and an increasing number of nonpolar carbon atoms in the analyte decrease the matrix effect.…”

Section: Resultsmentioning

confidence: 99%

“…Both polarity and stability of a compound would be important factors influencing the degree of response enhancement caused by the matrix-like effect. It has been shown that highly polar compounds, such as organophosphates, have a high adsorption interaction potential with active sites, and a fraction of these decompose in Information Table S3) Note: All tested solutions contained all four IS candidates at the same concentration as the evaluated pesticide (0.05 or 0.5 μg mL −1 each) model for the matrix effect in GC analysis using quantitative structure-property relationships [30]. They concluded that increases in molecular polarity (particularly H-bond donors) and the volume of the analyte increase the matrix effect, while hydrophobicity and an increasing number of nonpolar carbon atoms in the analyte decrease the matrix effect.…”

Section: Compoundsmentioning

confidence: 99%

Evaluation of the matrix-like effect in multiresidue pesticide analysis by gas chromatography with tandem mass spectrometry

et al. 2017

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In multiresidue pesticide analysis using gas chromatography, it has long been recognized that an increase in the number of pesticides present in a standard solution can result in an enhancement of the peak responses of certain pesticides. Despite being widely acknowledged, this phenomenon has been rarely studied and is poorly understood. In this study, the authors have tentatively called this phenomenon the "matrix-like effect" and demonstrated it clearly using gas chromatography with tandem mass spectrometry. Five selected pesticides, namely, omethoate, terbufos, malathion, procymidone, and permethrin, and four internal standard candidates, namely, triphenyl phosphate, naphthalene-d , phenanthrene-d , and fluoranthene-d , were used to evaluate the matrix-like effect following the addition of 58, 108, and 166 other pesticides. With the exception of naphthalene-d , the responses of all evaluated pesticides and internal standard candidates were dramatically enhanced by the addition of up to 166 coexisting pesticides. The relative response factors of the five pesticides to each internal standard candidate were not constant under the conditions studied, meaning that these internal standard candidates did not adequately compensate for the matrix-like effect, at least for the five evaluated pesticides. The results revealed that the presence of various mixtures of pesticides in standard solutions might act as an unintentional analyte protectant, that is, some sort of troublesome "quasi-matrix."

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“…were compared . The relationship between the matrix effects and physicochemical properties was investigated, and it indicated that the molecular polarity and volume of the analyte increase the matrix effect, while hydrophobicity and an increasing number of nonpolar carbon atoms in the analyte decrease the matrix effects . Nevertheless, further research should be performed to clarify the origin and mechanism underlying these matrix effects.…”

Section: Resultsmentioning

confidence: 99%

A quick, easy, cheap, effective, rugged, and safe method for the simultaneous detection of four triazolone herbicides in cereals combined with ultrahigh performance liquid chromatography with tandem mass spectrometry

Yan

Liu

et al. 2014

J of Separation Science

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This paper describes a novel, rapid, and sensitive analytical method for monitoring four triazolone herbicides in cereals (wheat, rice, corn, and soybean), using a quick, easy, cheap, effective, rugged, and safe sample extraction procedure followed by ultrahigh performance liquid chromatography coupled with tandem mass spectrometry. The four triazolone herbicides (amicarbazone, carfentrazone-ethyl, sulfentrazone, and thiencarbazone-methyl) were extracted using acidified acetonitrile (containing 1% v/v formic acid) and subsequently purified with octadecylsilane (C18 ) prior to sample analysis. Ultrahigh performance liquid chromatography coupled with tandem mass spectrometry was operated in positive and negative ionization switching mode. Amicarbazone and carfentrazone-ethyl were detected in the positive mode (ESI+), while sulfentrazone and thiencarbazone-methyl were detected in the negative mode (ESI-). All compounds were successfully separated in less than 3.0 min. Further optimization achieved desired recoveries ranging from 74.5 to 102.1% for all analytes with relative standard deviation values ≤17.2% in all tested matrices at three levels (10, 100, and 500 μg/kg). The limits of detection for all compounds were ≤2.3 μg/kg, and the limits of quantitation did not exceed 7.1 μg/kg. The developed method showed excellent linearity (R(2) ≥ 0.994) and was proven to be highly efficient and reliable for the routine monitoring of triazolone herbicides in cereals.

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Relationship between the matrix effect and the physicochemical properties of analytes in gas chromatography

Cited by 13 publications

References 29 publications

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

Evaluation of the matrix-like effect in multiresidue pesticide analysis by gas chromatography with tandem mass spectrometry

A quick, easy, cheap, effective, rugged, and safe method for the simultaneous detection of four triazolone herbicides in cereals combined with ultrahigh performance liquid chromatography with tandem mass spectrometry

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