Relationship between the Amounts of Antibodies to <i>Actinobacillus pleuropneumoniae</i> Serotype 2 Detected in Blood Serum and in Fluids Collected from Muscles of Pigs

Wallgren, Per; Persson, Maurits

doi:10.1111/j.1439-0450.2000.00408.x

Cited by 3 publications

(2 citation statements)

References 27 publications

(31 reference statements)

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“…Serum antibodies to M. hyopneumoniae and H. parasuis were analysed by commercial kits according to the manufacturers’ instructions (IDEXX M. hyo . Ab test, IDEXX, Westbrook, USA; Swinecheck HPS, Biovet, St Hyacinthe, Canada) and to A. pleuropneumoniae and P. multocida by indirect ELISA systems [ 30 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Ahlberg

Hjertner

Wallgren

et al. 2017

Vet Res

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Saponin-based adjuvants have been widely used to enhance humoral and cellular immune responses in many species, but their mode of action is not fully understood. A characterization of the porcine transcriptional response to Matrix-M was performed in vitro using lymphocytes, monocytes or monocyte-derived dendritic cells (MoDCs) and in vivo. The effect of Matrix-M was also evaluated in specific pathogen free (SPF) pigs exposed to conventionally reared pigs. The pro-inflammatory cytokine genes IL1B and CXCL8 were up-regulated in monocytes and lymphocytes after Matrix-M exposure. Matrix-M also induced IL12B, IL17A and IFNG in lymphocytes and IFN-α gene expression in MoDCs. Several genes were indicated as up-regulated by Matrix-M in blood 18 h after injection, of which the genes for IFN-α and TLR2 could be statistically confirmed. Respiratory disease developed in all SPF pigs mixed with conventional pigs within 1–3 days. Two out of four SPF pigs injected with saline prior to contact exposure displayed systemic symptoms that was not recorded for the four pigs administered Matrix-M. Granulocyte counts, serum amyloid A levels and transcription of IL18 and TLR2 coincided with disease progression in the pigs. These results support further evaluation of Matrix-M as a possible enhancer of innate immune responses during critical moments in pig management.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-017-0437-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Ahlberg

Hjertner

Wallgren

et al. 2017

Vet Res

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“…An indirect ELISA, based on a phenol-water extraction of the microbe as coating antigen, was used to measure serum antibodies to A. pleuropneumoniae serotype 2 in all serum samples collected. The cut-off value for a positive reaction in sera diluted 1/1000 was defined as A 450 = 0.5 [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Field experience with two different vaccination strategies aiming to control infections with Actinobacillus pleuropneumoniae in a fattening pig herd

Sjölund

Wallgren

2010

Acta Vet Scand

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BackgroundThe prevalence of pleurisies recorded at slaughter is increasing in Sweden, and acute outbreaks of actinobacillosis that require antimicrobial treatments have become more frequent. As an increased use of antimicrobials may result in the development of antimicrobial resistance it is essential to develop alternative measures to control the disease. Vaccinations present an appealing alternative to antimicrobial treatments. The aim of this work was to evaluate the potential of two different vaccination strategies in a specialized fattening herd affected by actinobacillosis.MethodsThe study was conducted in a specialized fattening herd employing age segregated rearing in eight units. The herd suffered from infections caused by Actinobacillus pleuropneumoniae serotype 2, confirmed by necropsy and serology. The study included 54 batches of pigs grouped into five periods. Batches of pigs of the second period were vaccinated against actinobacillosis twice, and pigs in the fourth period were vaccinated three times. Batches of pigs of the first, third and fifth period were not vaccinated. Concentrations of serum antibodies to A. pleuropneumoniae and serum amyloid A (SAA) were analysed and production data were recorded.ResultsDespite vaccinating, medical treatments were required to reduce the impact of the disease. The mean incidence of individual treatments for respiratory diseases during the rearing period ranged from 0 to 4.7 ± 1.8%, and was greatest during the triple vaccination period (period IV; p < 0.05 when compared to other groups). A large proportion of the vaccinated pigs seroconverted to A. pleuropneumoniae serotype 2 in the absence of a SAA-response. The prevalence of pleuritis decreased from 25.4 ± 6.5% in the first period to 5.0 ± 3.7% in the fifth period (p < 0.001).ConclusionsThe vaccine did not effectively prevent clinical expression of A. pleuropneumoniae infections, but seroconversion to A. pleuropneumoniae in the absence of a SAA-response in a large number pigs indicated that the vaccine had activated the immune system. Further, the prevalence of pleuritis decreased with time. This indicates that vaccinations together with intensified medical treatments of affected pigs could be useful in reducing the impact of A. pleuropneumoniae serotype 2 infections.

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Actinobacillus pleuropneumoniaein Thai Pig Herds. Prevalence of Serum Antibodies and Relation to Performance

Assavacheep

Persson

Luengyosluechakul

et al. 2003

Journal of Veterinary Medicine, Series B

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The aim of the study was to investigate the prevalence of Actinobacillus pleuropneumoniae infections in market weight pigs in Thailand. ELISA systems employing purified lipopolysaccharide antigens were used to detect antibodies in 549 serum samples collected from pigs of 22 herds. Relevant cut-off values were established from three herds defined seronegative. Serum antibodies were detected to all serotypes except serotype 10. Almost 60% of the samples were seropositive to at least one serotype and 45% of the pigs were seropositive to more than one serotype. Antibodies to the cross-reacting serotypes 1, 9 or 11 were found in 29% of the pigs. Other common serotypes included the cross-reacting serotypes 3, 6 or 8 (26% seropositive pigs) and serotype 5a (also 26%). Antibodies to serotypes 2, 5b and 12 were low in prevalence (<10%). Three herds were regarded to be seronegative and six to have a low pathogen load with respect to the prevalence of seropositive pigs. The remaining 13 herds had a high incidence of pigs with antibodies to A. pleuropneumoniae, dominated by serotypes 1-9-11 and 5a (n = 6), serotypes 3-6-8, and 5a (n = 4) or 1-9-11, 3-6-8, 5a and 4-7 (n = 3). A low pathogen load with respect to A. pleuropneumoniae, as well as small herd size and age-segregated rearing, tended to improve the performance of growers.

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Relationship between the Amounts of Antibodies to Actinobacillus pleuropneumoniae Serotype 2 Detected in Blood Serum and in Fluids Collected from Muscles of Pigs

Cited by 3 publications

References 27 publications

Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Field experience with two different vaccination strategies aiming to control infections with Actinobacillus pleuropneumoniae in a fattening pig herd

Actinobacillus pleuropneumoniaein Thai Pig Herds. Prevalence of Serum Antibodies and Relation to Performance

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