Relationship between super antigenicity, antimicrobial resistance and origin of Staphylococcus aureus isolated

Arias, Luisa; Espinal, Jenna Samara Luligo; Ortiz, José Ignacio Moncayo; Ibarra, Jorge Javier Santacruz; Aldana, Adalucy Álvarez

doi:10.25100/cm.v47i1.1818

Cited by 7 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, we determined that the egc operon is frequently found in the 13 analyzed strains, in concordance with an extensive prospective study in Colombia (74). In contrast to this study, our results in the analyzed strains demonstrated that sen (previously sek) was more frequent than the operon itself.…”

Section: Discussionsupporting

confidence: 88%

egc Superantigens Impair Monocytes/Macrophages Inducing Cell Death and Inefficient Activation

et al. 2020

View full text Add to dashboard Cite

Bacterial superantigens (SAgs) are enterotoxins that bind to MHC-II and TCR molecules, activating as much as 20% of the T cell population and promoting a cytokine storm which enhances susceptibility to endotoxic shock, causing immunosuppression, and hindering the immune response against bacterial infection. Since monocytes/macrophages are one of the first cells SAgs find in infected host and considering the effect these cells have on directing the immune response, here, we investigated the effect of four non-classical SAgs of the staphylococcal egc operon, namely, SEG, SEI, SEO, and SEM on monocytic-macrophagic cells, in the absence of T cells. We also analyzed the molecular targets on APCs which could mediate SAg effects. We found that egc SAgs depleted the pool of innate immune effector cells and induced an inefficient activation of monocytic-macrophagic cells, driving the immune response to an impaired proinflammatory profile, which could be mediated directly or indirectly by interactions with MHC class II. In addition, performing surface plasmon resonance assays, we demonstrated that non-classical SAgs bind the gp130 molecule, which is also present in the monocytic cell surface, among other cells.

show abstract

Section: Discussionsupporting

confidence: 88%

egc Superantigens Impair Monocytes/Macrophages Inducing Cell Death and Inefficient Activation

et al. 2020

View full text Add to dashboard Cite

show abstract

Investigation of Virulence Genes of Staphylococcus aureus Isolated from Sterile Body Fluid Samples and Their Correlation with Clinical Symptoms and Outcomes

Lin

Jin

et al. 2021

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Staphylococcus aureus is the major pathogen causing nosocomial human infections and produces a variety of virulence factors that contribute to its ability to colonize and cause diseases. This study was conducted to investigate the virulence genes in S. aureus isolated from sterile body fluid samples and their correlation with clinical symptoms and outcomes. The VITEK 2® Compact system was used to perform biochemical identification and antimicrobial susceptibility tests on 33 S. aureus isolates. Virulence genes were amplified using multiplex PCR. The virulence gene patterns were analyzed by systematic cluster analysis. The frequency of methicillin-resistant S. aureus was 45.45%, and 17 virulence genes were identified. Genes encoding hemolysins showed high frequencies. The frequencies of hla, hlb, hld, and hlgB were 93.94% and that of the luk-F/S-PV was 21.21%. Except for the frequency of splB (51.52%), the remaining genes encoding invasive proteases showed frequencies greater than 81.82%. Among the patients, 100.00% had undergone invasive medical procedures and 24.00% had been treated with more than three types of antibiotic drugs. Invasive medical procedures are the main causes of infection. Resistance to antibiotic drugs and the status of carrying virulence genes were highly related to clinical symptoms and outcomes.

show abstract

Association between the accessory gene regulator (agr) locus and the presence of superantigen genes in clinical isolates of methicillin-resistant Staphylococcus aureus

2019

View full text Add to dashboard Cite

ObjectiveMethicillin-resistant Staphylococcus aureus cause to a variety of hard to cure infections. MRSA isolates also, produce an arsenal of virulence factors contribute to severe infections. The aim of this study was to find out the relationship between agr locus and presence of S. aureus superantigens (SAgs).ResultsClinical isolates in two groups from two different states of Iran were collected. Antibiotic resistance patterns, agr typing, and virulence factor genes prevalence were identified and relationship between them was analyzed using SPSS software version16. Most of the samples were collected from wound 39 isolates in Group 1 and 61 isolates in Group 2. Frequency of MRSA strains was 38.1% in Group 1 and 52.1% in Group 2. Also, the most common resistance among both groups was to penicillin. agr positive isolates were detected in 132 isolates of Group 1 and 104 isolates of Group 2. In Conclusion, a significant relationship between the SAgs frequency and agr locus in both groups has been indicated. The production of superantigens in S. aureus plays an important role in the classification of agr locus, and this locus can affect differently in methicillin-resistant strains.Electronic supplementary materialThe online version of this article (10.1186/s13104-019-4166-7) contains supplementary material, which is available to authorized users.

show abstract

Relationship between super antigenicity, antimicrobial resistance and origin of Staphylococcus aureus isolated

Cited by 7 publications

References 30 publications

egc Superantigens Impair Monocytes/Macrophages Inducing Cell Death and Inefficient Activation

egc Superantigens Impair Monocytes/Macrophages Inducing Cell Death and Inefficient Activation

Investigation of Virulence Genes of Staphylococcus aureus Isolated from Sterile Body Fluid Samples and Their Correlation with Clinical Symptoms and Outcomes

Association between the accessory gene regulator (agr) locus and the presence of superantigen genes in clinical isolates of methicillin-resistant Staphylococcus aureus

Contact Info

Product

Resources

About