Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, neutral invertase and soluble acid invertase in micropropagated sugarcane plants

Gutiérrez-Miceli, Federico Antonio; Rodríguez-Mendiola, M. A.; Ochoa-Alejo, Neftalí; Mendez-Salas, R.; Dendooven, Luc; Arias-Castro, Carlos

doi:10.1007/s11738-002-0041-5

Cited by 27 publications

(25 citation statements)

References 27 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data are mean ± SE with three biological replicates Planta (2011) 234:445-457 453 suggesting that the accumulated Pro is not a key factor for the observed greater tolerance of M. falcata to freezing. There have been numerous reports demonstrating that cold acclimation-induced increases in soluble sugars are positively correlated with freezing tolerance in a number of species (Castonguay et al 1995;Wanner and Junttila 1999;Gilmour et al 2000;Gutierrez-Miceli et al 2002;Klotke et al 2004). Our results demonstrated that soluble sugar and sucrose contents were significantly correlated with freezing tolerance in M. falcata, but not in M. truncatula (Fig.…”

Section: Discussionsupporting

confidence: 51%

“…Exogenous supply of sugars enhances freezing tolerance in a number of species (Tabaei-Aghdaei et al 2003). During cold acclimation, the accumulation of sucrose is related to increases in activities of SPS and SuSy, while sucrose content is negatively regulated by the activity of acid invertase (Gutierrez-Miceli et al 2002;Bhowmik et al 2006). In addition, sugars such as sucrose and glucose may also play a role in regulation of cold-induced expression of genes in plants (Tabaei-Aghdaei et al 2003).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Comparative studies on tolerance of Medicago truncatula and Medicago falcata to freezing

Zhang

Zhao

Tian

et al. 2011

Planta

101

View full text Add to dashboard Cite

Medicago falcata is a legume species that exhibits great capacity of tolerance to abiotic stresses. To elucidate the mechanism underlying tolerance of M. falcata to freezing, we compared the characteristics of M. falcata in response to cold acclimation and freezing with those of the legume model plant Medicago truncatula. M. falcata seedlings were more tolerant to freezing than M. truncatula, as evidenced by a lower value of EL(50) (temperature at which 50% electrolyte leakage after freezing) and greater survival rate for M. falcata than M. truncatula. Cold acclimation led to greater reduction in EL(50) for M. falcata than M. truncatula. Sucrose was the most abundant sugar in both M. falcta and M. truncatula, and a greater accumulation of sucrose and Pro in M. falcata than in M. truncatula during cold acclimation was observed. Cold acclimation induced small amounts of raffinose and stachyose in M. falcata, but not in M. truncatula. The activities of sucrose phosphate synthase and sucrose synthase were greater in M. falcata than in M. truncatula. In contrast, the activity of acid invertase was higher in M. truncatula than in M. falcata. There was an increase in transcript of CRT binding factor (CBF) upon exposure to low temperature in the two species. The low temperature-induced increase in transcript of CBF2 was much higher in M. truncatula than in M. falcata, while transcript of CBF3 in M. falcata was greater than that in M. truncatula. There were sustained increases in transcripts of cold acclimation specific (CAS), a downstream target of CBF, during cold acclimation and the increases were greater in M. falcata than in M. truncatula. These results demonstrate that accumulation of greater amounts of soluble sugars coupled with higher CBF3 and CAS transcript levels in M. falcata may play a role in conferring greater tolerance of M. falcata to freezing than that of M. truncatula.

show abstract

Section: Discussionsupporting

confidence: 51%

mentioning

confidence: 99%

Comparative studies on tolerance of Medicago truncatula and Medicago falcata to freezing

Zhang

Zhao

Tian

et al. 2011

Planta

101

View full text Add to dashboard Cite

show abstract

“…The extraction and enzyme assays were done as described by Gutiérrez-Miceli et al (2002). Briefly, SPS and SUSY were extracted, dialyzed, partially purified on a micro-scale ω-aminohexyl-agarose column as described by Dancer et al (1990) and recovered by washing with 500 mM NaCl solution.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Sucrose accumulation and enzyme activities in callus culture of sugarcane

Gutiérrez-Miceli¹,

Rodríguez-Mendiola²,

Ochoa-Alejo³

et al. 2005

Biol. plant.

View full text Add to dashboard Cite

The activities of sucrose phosphate synthase (SPS), sucrose synthase (SUSY), neutral invertase (NI) and soluble acid invertase (SAI) were measured in callus cultures of four Mexican sugarcane cultivars (Saccharum spp.) with a different capacity to accumulate sucrose in stem parenchyma cells. The results indicated that sucrose accumulation in callus was positively correlated to the activity of SPS and SUSY and negatively to the activity of SAI and NI while SPS explained most of the variation found for sucrose accumulation and NI least.Additional key words: neutral invertase, Saccharum spp., soluble acid invertase, sucrose phosphate synthase, sucrose synthase.

show abstract

“…Generally, carbon metabolism starts from the breakdown of sucrose during canola seed filling (White et al 2000). Invertase and sucrose synthase (SUS) are two enzymatic systems that catalyze sucrose in consequential metabolism (Schmalstig and Hitz 1987;Gutiérrez-Miceli et al 2002;Fallahi et al 2008). In canola, considerable amounts of hexose accumulate in the early developing silique and seed (mainly in the form of liquid endosperm) (King et al 1997;Hill et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Comparison on the carbohydrate metabolic enzyme activities and their gene expression patterns in canola differing seed oil content

Hua

Zhang

et al. 2015

Plant Growth Regul

View full text Add to dashboard Cite

Carbohydrates are an important regulator of canola seed oil content. However, understanding the physiological regulation by carbohydrates governing seed oil accumulation is fragmented. In the present study, activities of sucrose and starch catalytic enzymes, including neutral and alkaline invertase, sucrose synthase (SUS), and starch phosphorylase, and biosynthetic enzymes, including sucrose phosphate synthase and ADP-glucose pyrophosphorylase, were compared in developing silique and seed of high oil content and low oil content lines (HOCL and LOCL, respectively). The results showed that the HOCL had significantly higher total soluble sugar concentration in the developing silique wall and seed during the seed lipid accumulation stage. Strikingly, all the enzymes showed very strong activities at 20 days after anthesis in the silique wall of the HOCL, which was in agreement with the higher amounts of corresponding gene expression. The result indicated that the homeostasis of the high efficiency of cleavage and biosynthetic of carbohydrates in the HOCL was beneficial for the rapid volume expansion of silique and transportation of carbohydrates, i.e., sucrose and starch, to the seed for utilization. At seed deposition stage, all the enzymes exhibited significantly higher activities in the HOCL than in the LOCL, which was helpful for increased production of carbohydrates. The results of gene expression at seed oil deposition stages revealed that one of the SUS genes, SUS3, showed significantly higher transcript amount in the HOCL than in the LOCL at all stages while most of other genes showed no significant differences between two lines at developing stages. Therefore, SUS3 might be played vital roles in the sucrose cleavage.

show abstract

Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, neutral invertase and soluble acid invertase in micropropagated sugarcane plants

Cited by 27 publications

References 27 publications

Comparative studies on tolerance of Medicago truncatula and Medicago falcata to freezing

Comparative studies on tolerance of Medicago truncatula and Medicago falcata to freezing

Sucrose accumulation and enzyme activities in callus culture of sugarcane

Comparison on the carbohydrate metabolic enzyme activities and their gene expression patterns in canola differing seed oil content

Contact Info

Product

Resources

About