Sucrose accumulation and enzyme activities in callus culture of sugarcane

Gutiérrez-Miceli, Federico Antonio; Rodríguez-Mendiola, M. A.; Ochoa-Alejo, Neftalí; Mendez-Salas, R.; Arias-Castro, Carlos; Dendooven, Luc

doi:10.1007/s10535-005-0034-5

Cited by 20 publications

(12 citation statements)

References 16 publications

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“…The similar patterns of sugar accumulation were also shown in banana, kiwifruit and citrus (Langenkämper et al, 1998;Komatsu et al, 1999;Ménager et al, 2004). The contrary findings of SPS activity in other fruits indicated that different biochemical pathways participated into sugar accumulation (Gutierrez-Miceli et al, 2005;Grof et al, 2006).…”

Section: Sugar Accumulation and Metabolic Enzymes Analysissupporting

confidence: 55%

Physico-chemical properties of longan fruit during development and ripening

Shi

Wang

Liu

et al. 2016

Scientia Horticulturae

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Section: Sugar Accumulation and Metabolic Enzymes Analysissupporting

confidence: 55%

Physico-chemical properties of longan fruit during development and ripening

Shi

Wang

Liu

et al. 2016

Scientia Horticulturae

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“…; Gutierrez‐Miceli et al . ) and shown to have a pivotal role in carbohydrate metabolism. Usually, sucrose synthesised in the cytosol is partitioned either for export or for storage in the vacuole (Winter et al .…”

Section: Introductionmentioning

confidence: 99%

“…Acid invertase exists as a soluble form in the vacuole (SAI) and as a cell wall-bound (CWI) form, but is totally absent from the cytosol (Walker & Pollock 1993). The role of invertase, especially SAI, in regulating the sucrose content in leaves has been extensively studied (Scholes et al 1996;Gutierrez-Miceli et al 2005) and shown to have a pivotal role in carbohydrate metabolism. Usually, sucrose synthesised in the cytosol is partitioned either for export or for storage in the vacuole (Winter et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Expression analysis of genes associated with sucrose accumulation in sugarcane (Saccharum spp. hybrids) varieties differing in content and time of peak sucrose storage

et al. 2015

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Sucrose synthesis/accumulation in sugarcane is a complex process involving many genes and regulatory sequences that control biochemical events in source-sink tissues. Among these, sucrose synthase (SuSy), sucrose phosphate synthase (SPS), soluble acid (SAI) and cell wall (CWI) invertases are important. Expression of these enzymes was compared in an early (CoJ64) and late (BO91) maturing sugarcane variety using end-point and qRT-PCR. Quantitative RT-PCR at four crop stages revealed high CWI expression in upper internodes of CoJ64, which declined significantly in both top and bottom internodes with maturity. In BO91, CWI expression was high in top and bottom internodes and declined significantly only in top internodes as the crop matured. Overall, CWI expression was higher in CoJ64 than in BO91. During crop growth, there was no significant change in SPS expression in bottom internodes in CoJ64, whereas in BO91 it decreased significantly. Apart from a significant decrease in expression of SuSy in mature bottom internodes of BO91, there was no significant change. Similar SAI expression was observed with both end-point and RT-PCR, except for significantly increased expression in top internodes of CoJ64 with maturity. SAI, being a major sucrose hydrolysing enzyme, was also monitored with end-point PCR expression in internode tissues of CoJ64 and BO91, with higher expression of SAI in BO91 at early crop stages. Enzyme inhibitors, e.g. manganese chloride (Mn(++) ), significantly suppressed expression of SAI in both early- and late-maturing varieties. Present findings enhance understanding of critical sucrose metabolic gene expression in sugarcane varieties differing in content and time of peak sucrose storage. Thus, through employing these genes, improvement of sugarcane sucrose content is possible.

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“…However, as the primary translocatable carbohydrate in the majority of plants, the accumulation of sucrose is vital to the initiation of increased freezing tolerance (Strand et al 2003). Enzymes, such as sucrose-phosphate synthase, sucrose synthase, neutral invertase and soluble acid invertase, are directly involved in sucrose synthesis and degradation (Gutierrez-Miceli et al 2005). The alterations of these enzymes have been investigated in plants exposed to low temperatures; however, the activities of these enzymes at low temperatures vary greatly among plant species (Guy et al 1992;Tognetti et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Seasonal changes in non-structural carbohydrates and sucrose metabolism enzymes in two Sabina species

et al. 2011

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Evergreen plants need to store reserves to allow for their survival during the winter months and for new leaf growth in the following spring. In many of the tree species, these reserve functions are mainly carried out by starch, which is degraded to soluble carbohydrates during the dormant season to maintain active respiration and provide protection against freezing. In the present study, two evergreen woody plants, S. przewalskii Kom. (SP) and S. chinensis (Lin.) Ant.(SC), were used to investigate the patterns of seasonal variation in the concentrations of soluble sugars, sucrose, fructose and starch, and the activities of sucrose-phosphate synthase (SPS), sucrose synthase (SUSY), neutral invertase (NI) and soluble acid invertase (SAI). Foliar soluble sugar, sucrose, fructose, and starch concentrations were markedly higher in SC than in SP; moreover, the activities of SPS, SUSY, NI and SAI were also higher in SC than in SP. There were generally higher concentrations of soluble sugars, sucrose and fructose and SPS activities in the winter than in the summer for both Sabina trees, which was consistent with the necessity for protection against freezing; however, opposite results were found with regards to starch concentrations and the activities of SUSY, NI and SAI. In contrast with the activities of SUSY, NI and SAI, the negative correlation observed between SPS and air temperature was likely a reflection of its crucial role in the acquisition of freezing tolerance by sucrose metabolism in the winter. These results suggest that higher carbon reserves do not give S. chinensis (Lin.) Ant. a competitive advantage in tolerating cold temperatures and that only SPS, but not SUSY, NI and SAI, may play a positive role in freezing tolerance by increasing soluble sugar.

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Sucrose accumulation and enzyme activities in callus culture of sugarcane

Cited by 20 publications

References 16 publications

Physico-chemical properties of longan fruit during development and ripening

Physico-chemical properties of longan fruit during development and ripening

Expression analysis of genes associated with sucrose accumulation in sugarcane (Saccharum spp. hybrids) varieties differing in content and time of peak sucrose storage

Seasonal changes in non-structural carbohydrates and sucrose metabolism enzymes in two Sabina species

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