Relationship Between Salivary and Serum Testosterone Levels in Response to Different Exercise Intensities

Lane, Amy R.; VanBruggen, Mitch; Ondrak, Kristin S.; Hackney, Anthony C.

doi:10.1249/01.mss.0000494377.26447.fc

Cited by 2 publications

(11 citation statements)

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“…[14,19,40] In contrast to the recent study of Lippi et al, [45] our data revealed a higher correlation between total serum to salivary testosterone (r = 0.605) than between free serum and salivary testosterone (r = 0.379). The results showed a correlation between serum total and free testosterone, urinary and salivary testosterone absolute concentrations, and time-matched data, which confirms the results of other studies also in combination with exercise.…”

Section: Hormone Quantificationcontrasting

confidence: 99%

“…This result might be in contrast to other authors who have shown an increase of testosterone after acute resistance exercise. [19] So these factors could lead to the non-significant impact of physical exercise in the present study, where an all-out-incremental test on the cycle ergometer represents the exercise bout. Spearman rank correlation (Spearman's ρ) between the absolute concentrations (ρ absolute ) and relative change to control (ρ relative) of salivary testosterone and free testosterone, total testosterone and SHGB in serum, urinary testosterone and the salivary testosterone concentration measured by GC-MS/MS and LC-MS/MS.…”

Section: Hormone Quantificationmentioning

confidence: 93%

“…[19,25] The statistical analysis was performed using the automated statistical software tool SigmaStat 3.5 for Windows Software (Systat Software Inc, San Jose, CA, USA). Therefore, only urine and saliva concentrations were used for further analyses.…”

Section: Data Analysis and Statisticsmentioning

confidence: 99%

“…[33] The baseline measurements without any intervention and the measurements with exercise intervention only, did not provoke any significant changes in salivary, serum, and urinary testosterone concentrations, both in absolute concentrations and in timematched results. Although several authors [19,25,37] have shown that testosterone also has increased after endurance exercise at different intensities, the time of sampling as well as the intensity and duration of endurance exercise could interfere with testosterone levels. [34][35][36] But in the present study, we used a cycling ergometry Table 3.…”

Section: Hormone Quantificationmentioning

confidence: 99%

“…[10] Kley [11] even describes AAS as a lifestyle drug with a large user group and an associated illegal market. [7,18] Enzymelinked immunosorbent assays (ELISA) [19] was found to be a suitable alternative for clinical testing of testosterone concentration in saliva. [12] In order to verify testosterone levels, urine and serum samples are commonly used.…”

Section: Introductionmentioning

confidence: 99%

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Potential detection of low‐dose transdermal testosterone administration in blood, urine, and saliva

Schönfelder

Hofmann

Schulz

et al. 2016

Drug Testing and Analysis

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Administration of low amounts of endogenous hormones - so-called micro-dosages - are supposed to represent a major challenge in doping analysis. To model such a situation, we have studied transdermal administrations of 2.4 mg/24 h testosterone patches and examined various steroid concentrations in blood, urine, and saliva of 11 volunteers. Multiple samples were collected at t = 0, 3, 6, 9, 24, 48, and 72 h in four different phases, i.e., all combinations with/without physical exercise and with/without testosterone. Testosterone was analyzed by enzyme-linked-immuno-assay as well as by mass spectrometry and validated in an accredited anti-doping laboratory. Circadian controls with and without exercise did not provoke prominent alterations of whole, free, and salivary testosterone. Testosterone application for 24 h led to a significant (all p < 0.001) mean increase above controls: total testosterone (median: 5.2 vs. 8.0 ng/mL), free testosterone (median: 11.3 vs. 15.6 pg/mL), and salivary testosterone (median: 62.4 vs. 99.9 pg/mL). Additionally, all three testosterone measurements indicated significant correlations to each other (all r > 0.538, all p < .001). Circadian-matching showed peaking testosterone values after 6 h and 9 h, reaching highest augmentation up to 252.6 ± 123.5% in saliva after 9 h. After removal of the testosterone patch, all testosterone levels in blood, saliva, and urine returned to baseline within 24 h. Different techniques of hormone detection (enzyme-linked immunosorbent assay (ELISA), gas chromatography-tandem mass spectrometry (GC-MS/MS), liquid chromatography-tandem mass spectrometry (LC-MS/MS)) indicated significant correlations. Results indicate that saliva, blood, and urine exhibit comparable hormone augmentation during micro-dose testosterone application, indicating a possible consideration in future doping analysis. The inter-individual variability was high in all biofluids, requiring the use of an individual biological passport rather than statistical values. Copyright © 2016 John Wiley & Sons, Ltd.

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