Relationship Between Red Cell Bound Antibody
and Agglutination in the Antiglobulin Reaction

Dupuy, Mary Edith; Elliot, Margaret; Masouredis, S. P.

doi:10.1159/000464945

Cited by 17 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One, exemplified by cells agglutinated with IgM antibodies, protease modification of the red cell, and the antiglobulin reaction, involves the clustering of antigen receptors. The (7,17) has, in fact, shown that the antiglobulin reaction is capable of agglutinating cells that contain only 100-500 molecules of cell-bound IgG.…”

Section: Discussionmentioning

confidence: 99%

The role of antigen mobility in anti-Rh0(D)-induced agglutination.

Victoria¹,

Muchmore²,

Sudora³

et al. 1975

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

The role of antigen mobility in anti-Rh0(D)-induced agglutination.

Victoria¹,

Muchmore²,

Sudora³

et al. 1975

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…As the data of Fig. 6 (4,35). Although the dilution at which antiglobulin sera are useful in Coombs testing corresponds to their anti-IgG or anti-complement content, the greatest dilution of a single serum which agglutinates sensitized RBC has often been used as a "Coombs titer" and related to the intensity of red cell coating (36).…”

Section: Discussionmentioning

confidence: 99%

The significance of erythrocyte antigen site density

Hoyer¹,

Trabold²

1970

J. Clin. Invest.

View full text Add to dashboard Cite

“…An tiglobulin sera will cause obvious agglutina tion of RBCs, sensitized in vitro with IgG Rh antibodies, when there are more than 200 IgG molecules per RBC [4][5][6]; it has been reported that down to 120 IgG molecules/RBC can be detected by the AGT, if [7,8]. If more sensitive assays are used, then RBC-bound IgG can be shown to be present on the RBCs of most individuals.…”

Section: Do Circulating Blood Cells Of Healthy Individuals Have Igg Amentioning

confidence: 99%

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Garratty¹

1991

Gerontology

View full text Add to dashboard Cite

Normal circulating red blood cells (RBCs) and platelets have been shown to have small amounts of IgG on their membranes. The cell-bound IgG may be cytophilic (IgG nonspecifically adsorbed from the plasma) and/or IgG autoantibody. It has been suggested that most of the RBC-bound IgG is on older RBCs and is an autoantibody directed against senescent cell antigen (SCA). The accumulation of this RBC-bound IgG leads to Fc-dependent removal of senescent RBCs by macrophages in the reticuloendothelial system. RBCs also have complement components on their membrane; it is not clear how this accumulates on RBCs and whether it has a physiologic function. This small amount of nonpathogenic RBC-bound IgG is not detected by the antiglobulin test. It is still unclear whether the major difference between pathogenic and nonpathogenic IgG autoantibodies is qualitative, quantitative, or both. Seemingly healthy blood donors (1 in 1,000 donors) and patients without hemolytic anemia (up to 8% of patients) have increased amounts ( > 200 molecules/RBC) of RBC-bound IgG and complement that is detected by the antiglobulin test. This RBC-bound IgG has been shown to be an IgG autoantibody directed against blood group antigens, and/or IgG anti-idiotype, and/or IgG nonspecifically adsorbed onto the RBC membrane when plasma IgG levels are high. Most patients with autoimmune hemolytic anemia (AIHA) have RBC-bound IgG and/or complement detectable by the antiglobulin test. Most of the RBC-bound IgG is of the IgG1 subclass, whether one examines the RBCs of healthy blood donors or hospitalized patients with and without AIHA. Although the quantity of RBC-bound IgG is generally higher in patients with AIHA, there is no clear correlation with the quantity of RBC-bound IgG and the rate of in vivo RBC destruction. There is some recent evidence that the SCA autoantibody may at times be pathogenic and cause autoimmune disease.

show abstract

Relationship Between Red Cell Bound Antibody and Agglutination in the Antiglobulin Reaction

Cited by 17 publications

References 3 publications

The role of antigen mobility in anti-Rh0(D)-induced agglutination.

The role of antigen mobility in anti-Rh0(D)-induced agglutination.

The significance of erythrocyte antigen site density

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Contact Info

Product

Resources

About