The significance of erythrocyte antigen site density

The role of antigen mobility in anti-Rh0(D)-induced agglutination.

“…Analysis of the hemagglutination reaction in the past has emphasized the role of immunoglobulin class (40,50), the effect of quantity of cell-bound anti-body (15,41), and the role of electrochemical effects both with respect to antibody binding and to the subsequent agglutination of antibody-coated cells (38). More recently, however, evidence has been accumulating that suggests that freedom of translational mobility for membrane components is a fundamental property of cell surfaces (9,45).…”

The significance of erythrocyte antigen site density

“…Calcium and magnesium were omitted from the VBS for all studies other than immune hemolysis. RESULTS Covalent coupling of the sulfanilic acid groups to red cells has been shown to have minimal effect on the membrane properties (3,14). In a contrast with the unchanged agglutination properties of coupled red cells (3), a consistent dose-related increase in hemolysis was recognized (Table I).…”

“…Red cells coupled with sulfanilic acid were trace-labeled with 'Cr (E. R. Squibb & Sons, New York) to monitor cell recovery in the binding experiments (3). The cells were suspended in VBS to which had been added 2% (v/v) heat-inactivated normal human AB serum, and the concentration adjusted to 108 RBC/ml using a Coulter counter (Coulter Electronics Inc., Hialeah, Fla.).…”

Ectogalactosyltransferase. Presence of Enzyme and Acceptors on the Rat Lymphocyte Cell Surface

“…As the specific radioactivity of the UDP-['4C]Gal was 200 counts x min-' x pmol-' we evaluated the number of newly appeared external galactosyl residues as being at least 3 x lo4 per cell. It has been shown that the minimal number of receptors required for agglutination to take place with antibodies [16] or lectins (Sharon, personal communication) is around 2 x lo4 per cell. Therefore, spleen lymphocytes should acquire new agglutinating properties with soybean agglutinin after endogenous galactosylation of their outer cell surface.…”