Relationship between PGCs Settle and Gonad Development in the Early Chicken Embryo

Li, B. C.; Chen, G. H.; Xiao, X. J.; Qin, Jun-Hui; Wu, Shenglong; Xie, Ke-qin; Olojide, Olowokeso

doi:10.5713/ajas.2004.453

Cited by 9 publications

(11 citation statements)

References 10 publications

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“…We next further explored the specific function of Notch signaling during germ cell formation. To this end, we detected the formation of PGCs (4.5 days) [ 20 ] and SSCs (18 days) [ 21 , 22 ] after activation and inhibition of Notch signaling during chick embryo hatching. With respect to PGCs, we found that the activation of Notch signaling significantly upregulated the expression of Lin28 (8.3427 ± 0.23, p < 0.05) and Blimp1 (12.4213 ± 0.13, p < 0.05), which mark formation of PGCs at 4.5 days.…”

Section: Resultsmentioning

confidence: 99%

NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

et al. 2018

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BackgroundNotch signaling is mainly regulated by Notch1 during development of chicken germ stem cells; however, the molecular mechanisms that contribute to generation of these germ stem cells have not been thoroughly investigated.ResultsIn our studies, Overexpression of the Notch1 NICD promoted development of the reproductive ridge, but inhibited the formation of seminiferous tubules. The formation efficiency of PGCs in the reproductive ridge following overexpression of NICD (7.5% ± 0.11) was significantly higher than that (4.9% ± 0.17, p < 0.05) following inhibition of NICD, While the formation efficiency of spermatogonial stem cells (SSCs) in the testes (12.7% ± 0.08) was significantly lower after NICD overexpression than that after inhibition of NICD (16.3% ± 0.16, p < 0.05). Using co-immunoprecipitation, we found that this anomaly stemmed from the reversal of dissociation of the Notch-regulated transcription factor CBF-1/RBP co-suppression complex during the differentiation of PGCs into SSCs. This dissociation of the CBF-1/RBP co-suppressing complex during the differentiation of ESCs into PGCs resulted in the release of HDAC1 and HDAC2 and the recruitment of mastermind-like 1 to form a coactive complex to promote the expression of the downstream transcription suppressor hairy/enhancer of split-1. Dynamic expression of transducin-like enhancer of split 3, TLE4, and C-terminal binding protein 2 during further differentiation of PGCs inhibited the dissociation of the CBF-1/RBP co-suppression complex and inhibited the expression of the downstream genes.ConclusionsIn summary, Notch signaling plays diametrically opposing roles during normal development of chicken PGCs and SSCs, and these functions was determined by the expression of NICD, changes in the CBF-1/RBP complex composition, and histone modification.Electronic supplementary materialThe online version of this article (10.1186/s13578-018-0238-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

et al. 2018

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“…Separation and cultivation of ESCs, PGCs and SSCs were performed as previously described (Li et al . ; Sun et al . ).…”

Section: Methodsmentioning

confidence: 96%

“…The origin, migration, accumulation and settlement process of reproductive cells are well established (Li et al . ). Chickens also possess a unique advantage in their migration patterns, which make germ cells easily separable during early development.…”

Section: Introductionmentioning

confidence: 97%

Regulation of crucial lncRNAs in differentiation of chicken embryonic stem cells to spermatogonia stem cells

Wang

et al. 2016

Animal Genetics

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Regulation of crucial lncRNAs involved in differentiation of chicken embryonic stem cells (ESCs) to spermatogonia stem cells (SSCs) was explored by sequencing the transcriptome of ESCs, primordial germ cells (PGCs) and SSCs with RNA-Seq; analytical bioinformatic methods were used to excavate candidate lncRNAs. We detected expression of candidate lncRNAs in ESCs, PGCs and SSCs and forecasted related target genes. Utilizing wego, david and string, function and protein-protein interactions of target genes were analyzed. Finally, based on string analysis, interaction diagrams and relevant signaling pathways were established. Our results indicate a total of 9657 lncRNAs in ESCs, PGCs and SSCs, with 3549 defined as significantly different. We screened 20 candidate lncRNAs, each demonstrating a greater than eight-fold difference in |logFC| value between groups (ESCs vs. PGCs, ESCs vs. SSCs and PGCs vs. SSCs) or specifically expressed in an individual cell type. qRT-PCR results indicated that expression tendencies of candidate lncRNAs were consistent with RNA-Seq. Fifteen cis and four trans target genes were forecasted. Based on wego and string analyses, we found lnc-SSC1, lnc-SSC5, lnc-SSC2 and lnc-ESC2 negatively regulated target genes SUFU, EPHA3, KLF3, ARL3 and TRIM8, whereas SHH, NOTCH, TGF-β, cAMP/cGMP and JAK/STAT signaling pathways were promoted, causing differentiation of ESCs into SSCs. Our findings represent a preliminary unveiling of lncRNA-associated regulatory mechanisms during differentiation of chicken ESCs into SSCs, filling a research void in male germ cell differentiation related to lncRNA. Our results also provide basic information for improving in vitro induction systems for differentiation of chicken ESCs into SSCs.

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“…A total of 18,340 eggs were used in this experiment, which were divided into three groups as three biological replicates. The eggs used for collection of embryonic stem cells (ESC) were used immediately, while those used for isolation of primordial germ cell (PGC) or isolation of spermatogonial stem cell (SSC) were incubated at 37°C and 75% relative humidity for 5.5 and 19 days, respectively [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Study on the Regulatory Mechanism of the Lipid Metabolism Pathways during Chicken Male Germ Cell Differentiation Based on RNA-Seq

Zuo

Zhang

et al. 2015

PLoS ONE

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Here, we explore the regulatory mechanism of lipid metabolic signaling pathways and related genes during differentiation of male germ cells in chickens, with the hope that better understanding of these pathways may improve in vitro induction. Fluorescence-activated cell sorting was used to obtain highly purified cultures of embryonic stem cells (ESCs), primitive germ cells (PGCs), and spermatogonial stem cells (SSCs). The total RNA was then extracted from each type of cell. High-throughput analysis methods (RNA-seq) were used to sequence the transcriptome of these cells. Gene Ontology (GO) analysis and the KEGG database were used to identify lipid metabolism pathways and related genes. Retinoic acid (RA), the end-product of the retinol metabolism pathway, induced in vitro differentiation of ESC into male germ cells. Quantitative real-time PCR (qRT-PCR) was used to detect changes in the expression of the genes involved in the retinol metabolic pathways. From the results of RNA-seq and the database analyses, we concluded that there are 328 genes in 27 lipid metabolic pathways continuously involved in lipid metabolism during the differentiation of ESC into SSC in vivo, including retinol metabolism. Alcohol dehydrogenase 5 (ADH5) and aldehyde dehydrogenase 1 family member A1 (ALDH1A1) are involved in RA synthesis in the cell. ADH5 was specifically expressed in PGC in our experiments and aldehyde dehydrogenase 1 family member A1 (ALDH1A1) persistently increased throughout development. CYP26b1, a member of the cytochrome P450 superfamily, is involved in the degradation of RA. Expression of CYP26b1, in contrast, decreased throughout development. Exogenous RA in the culture medium induced differentiation of ESC to SSC-like cells. The expression patterns of ADH5, ALDH1A1, and CYP26b1 were consistent with RNA-seq results. We conclude that the retinol metabolism pathway plays an important role in the process of chicken male germ cell differentiation.

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Relationship between PGCs Settle and Gonad Development in the Early Chicken Embryo

Cited by 9 publications

References 10 publications

NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

Regulation of crucial lncRNAs in differentiation of chicken embryonic stem cells to spermatogonia stem cells

Study on the Regulatory Mechanism of the Lipid Metabolism Pathways during Chicken Male Germ Cell Differentiation Based on RNA-Seq

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