2016
DOI: 10.1111/age.12510
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Regulation of crucial lncRNAs in differentiation of chicken embryonic stem cells to spermatogonia stem cells

Abstract: Regulation of crucial lncRNAs involved in differentiation of chicken embryonic stem cells (ESCs) to spermatogonia stem cells (SSCs) was explored by sequencing the transcriptome of ESCs, primordial germ cells (PGCs) and SSCs with RNA-Seq; analytical bioinformatic methods were used to excavate candidate lncRNAs. We detected expression of candidate lncRNAs in ESCs, PGCs and SSCs and forecasted related target genes. Utilizing wego, david and string, function and protein-protein interactions of target genes were an… Show more

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Cited by 10 publications
(5 citation statements)
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“…Moreover, we found that epigenetic regulation by addition of VC or VPA, as well as glycolytic inhibition with VK3, could significantly enhance PGC formation (from 12.2% to 16.41%). In addition, studies have shown that lncRNAs 42 , ubiquitination 43 , phosphorylation 44 , and histone methylation 45 promote iPGC formation, which should also be considered to optimize the iPGC induction system.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, we found that epigenetic regulation by addition of VC or VPA, as well as glycolytic inhibition with VK3, could significantly enhance PGC formation (from 12.2% to 16.41%). In addition, studies have shown that lncRNAs 42 , ubiquitination 43 , phosphorylation 44 , and histone methylation 45 promote iPGC formation, which should also be considered to optimize the iPGC induction system.…”
Section: Discussionmentioning
confidence: 99%
“…They are involved in the regulation of gene expression at different levels, affecting multiple cellular processes including cell differentiation, stem cell maintenance, and epithelial–mesenchymal transition 35. Accumulating evidence has strongly suggested that many lncRNAs function as oncogenes and/or tumor suppressors in tumor initiation and development 6.…”
Section: Introductionmentioning
confidence: 99%
“…A microRNA catalog of the developing post-oviposited chicken embryo provided an important foundation for further investigations on miRNA gene regulation in the chicken embryo ( Glazov, et al., 2008 ). Genome-wide identification of lncRNAs in embryonic skeletal muscle ( Li, et al., 2012 ; Zhenhui, et al., 2017 ), liver ( Ning, et al., 2020 ), and brain ( Xu, et al., 2018 ), and embryonic stem cells has also been performed ( Li, et al, 2017 ), suggesting lncRNA regulatory and functional roles in chicken embryonic development. These analyses show that noncoding RNAs constitute a substantial portion of the transcriptome and suggest that these play critical roles in animal embryogenesis.…”
Section: Discussionmentioning
confidence: 99%