Relationship between oxidative stress, lipid peroxidation, and ultrastructural damage in patients with coronary artery disease undergoing cardioplegic arrest/reperfusion

Milei, José; Forcada, Pedro; Fraga, César G.; Grana, Daniel; Iannelli, Gabriele; Chiariello, Massimo; Tritto, Isabella; Ambrosio, Giuseppe

doi:10.1016/j.cardiores.2006.12.007

Cited by 61 publications

(44 citation statements)

References 52 publications

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“…Only when defense mechanisms are overwhelmed oxidant attack progresses to cell injury. Likewise, Jansen et al [23] reported that human hearts subjected to cardioplegia and subsequent reperfusion show oxidative stress without an increase in lipid peroxidation, and Milei et al [24] found that in patients receiving elective bypass surgery, oxidative stress upon reperfusion after cardioplegic arrest doesn´t lead to reperfusion injury.…”

Section: Discussionmentioning

confidence: 99%

Markers of oxidative stress in acute myocardial infarction treated by percutaneous coronary intervention

et al. 2009

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AbstractIn the current study, we evaluated the dynamics of oxidative stress markers in patients with acute myocardial infarction (AMI) treated by primary percutaneous coronary intervention (PCI). Thirty consecutive patients with AMI with ST elevation were included. Plasma lipid peroxidation end product malondialdehyde (MDA) and total antioxidant capacity (TAC) in blood plasma were evaluated. Peripheral venous blood samples were obtained prior to reperfusion and at five time points after reperfusion. The control group consisted of 20 ischemic patients without acute coronary syndrome. TAC in the AMI group at admission was lower than in control patients (1.26 + 0.32 vs. 1.52 + 0.24 mmol/l). Within 1 h after reperfusion, in most cases, values significantly declined (1 min, 1.10 + 0.33 mmol/l; 1 h, 1.06 + 0.21 mmol/l [p= 0.03]). After 3 h, values began to increase (1.14 + 0.29 mmol/l) and returned to basal values after 3 d (1.29 + 0.24 mmol/l). MDA levels in AMI patients at admission were higher than in control patients (1.66 + 0.55 vs. 1.44 + 0.55 mmol/l) but showed a sustained decrease over the 3 h after reperfusion of the occluded artery (1 min, 1.57 + 0.37 mmol/l; 1 h, 1.50 + 0.35 μmol/l; 3 h, 1.35 + 0.59 μmol/l [p = 0.03]). Reperfusion of the occluded coronary artery by PCI in AMI lead to an immediate decrease in TAC, suggesting formation of reactive oxygen species. However, the MDA level significantly decreased after reperfusion. This may suggests less reperfusion injury after PCI.

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Section: Discussionmentioning

confidence: 99%

Markers of oxidative stress in acute myocardial infarction treated by percutaneous coronary intervention

et al. 2009

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“…The ROF production during the cardioplegic arrest is reduced by antioxidant enzymes [10,24] which are one of OS markers. In available publications related to myocardial ischemia/reperfusion injury, some of the most common markers used for the assessment of OS are: glutathione (GSH) [1,9,11], oxidized glutathione (GSSG) [1,7], manganese superoxide dismutase (MnSOD) [10,24], myeloperoxidase (MPO) [8] or thiobarbituric acid reactive substances (TBARS) like malondialdehyde (MDA) [8,11,14].…”

Section: Discussionmentioning

confidence: 99%

“…However, insignificant changes in right atrium auricles ultrastructure and a similar percentage of apoptotic cells before and after the surgery accompanied by good surgery results prove the effectiveness of the applied myocardial protection [7,8,11,26]. On this basis one may assume that in the case of cardiosurgical procedures with short aorta cross-clamping time (mean aorta cross-clamping time: 29.5min.…”

Section: Discussionmentioning

confidence: 99%

“…The oxidative reaction leads to cell malfunctions or even cells necrosis the consequence of which is organ insufficiency (the so called postoperative heart failure) [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Thus it is recommended especially in the case of patients with decreased left ventricle ejection fraction or in the case of patients with indications for an urgent procedure, e.g. recent infarct or instable angina [8,12,13]. Regardless of this, in meta-analyses encompassing both longan short-term observations, no significant differences in mortality, morbidity and life quality after surgical heart revascularisation were found [17,18].…”

Section: Introductionmentioning

confidence: 99%

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The estimation of oxidative stress markers and apoptosis in right atrium auricles cardiomyocytes of patients undergoing surgical heart revascularisation with the use of warm blood cardioplegia.

Nowicki

Saczko²,

Kulbacka³

et al. 2010

Folia Histochem Cytobiol.

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Abstract:Oxidative stress markers and apoptosis were estimated during elective surgical heart revascularization. Eight patients with good ejection fraction underwent coronary artery bypass grafting (CABG) with the use of warm blood cardioplegia. Two right atrium auricle biopsy specimens were collected before and after the operation. Specimens underwent immunocytochemical analysis of mitochondrial manganese superoxide dismutase (MnSOD) expression and apoptosis estimation by the TUNEL method. Ultrastructure analysis under electron microscope was made. Satisfactory results of the operation were obtained. After CABG the MnSOD expression increase in sections of auricles was observed through the increase of stain intensity and the percentage of cells with positive stain (from 30 to 80%). The apoptotic cells percentage remained at approximately the same level. Under the electron microscope insignificant pathological changes were observed. On this basis one may assume that in the case of cardiosurgical procedures with short aorta cross-clamping time and low operation risk level the application of cardioplegia sufficiently prevents reactive oxygen forms (ROF) cytotoxic activity although it does not inhibit the expression of oxidative stress (OS) markers. In our opinion the method of examining right atrium sections is safe and provides results comparable with other publications. It may also be a voice in the discussion on new methods of heart protection during cardiac surgery procedures.

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Dipteran endoparasitoid Exorista bombycis utilizes antihemocyte components against host defense of silkworm Bombyx mori

Moteriya

Hungund²,

Pradeep

2022

Arch Insect Biochem Physiol

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Dipteran endoparasitoids avoid host immune response; however, antidefense components from the Dipterans are unknown. Infestation of commercial silkworm Bombyx mori Linnaeus (Lepidoptera: Bombycidae) by endoparasitoid Exorista bombycis Louis (Diptera: Tachinidae) induced immune reactions, cytotoxicity, granulation, degranulation, and augmented release of cytotoxic marker enzyme lactate dehydrogenase (LDH), and degranulation‐mediator enzyme β‐hexosaminidase in hemocytes. In this study, by reverse phase high‐performance liquid chromatography, fractions of E. bombycis larval tissue protein with antihemocytic activity are separated. From the fraction, peptides of hemocyte aggregation inhibitor protein (HAIP) and pyridoxamine phosphate oxidase (PNPO) are identified by mass spectrometry. Interacting partners of HAIP and PNPO are retrieved that further enhance the virulence of the parasitoid. PNPO and HAIP genes showed a four‐ to seven fold increase in expression in the integument of the parasitoid larva. Together, the dipteran endoparasitoid E. bombycis exploit antihemocyte activity to inhibit host defense reactions in addition to defense evasion contemplated.

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Relationship between oxidative stress, lipid peroxidation, and ultrastructural damage in patients with coronary artery disease undergoing cardioplegic arrest/reperfusion

Abstract: Careful investigation reveals that while oxidant production does occur during cardiac surgery in patients with chronic ischemic heart disease, cardiac oxidative stress may not progress through membrane damage and irreversible injury.

Cited by 61 publications

References 52 publications

Markers of oxidative stress in acute myocardial infarction treated by percutaneous coronary intervention

Markers of oxidative stress in acute myocardial infarction treated by percutaneous coronary intervention

The estimation of oxidative stress markers and apoptosis in right atrium auricles cardiomyocytes of patients undergoing surgical heart revascularisation with the use of warm blood cardioplegia.

Dipteran endoparasitoid Exorista bombycis utilizes antihemocyte components against host defense of silkworm Bombyx mori

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