Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus

Sadiq, Chinar Hameed; Hussein, Ridha Hassan; Maulood, Ismail M.

doi:10.24996/ijs.2021.62.3.8

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Leptin is an adipokine that is secreted from adipose tissue and transported to the brain through blood brain barrier by leptin transporters called obesity receptors which stimulate the brain to reduce food intake, appetite body and regulate energy homeostasis.Leptin increases in obese persons [14].Also leptin receptor OBR expression increases to translocate the leptin to the brain to inform the brain about the status of body fat stores and induce anorexia that functions as an afferent signal in a negative feedback loop which maintains homeostatic control of adipose tissue mass [15].…”

Section: Resultsmentioning

confidence: 99%

Detection of Leptin and Ghrelin Hormones and the Expression of their Receptors in Iraqi Obese Individuals

Alsaffar,

Jumaa,

Baqer

2024

Iraqi Journal of Science

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Obesity is a complex disease and a major worldwide health hazard with adult mortality. Obesity is defined by an increase in the body-mass index of 30 kg m−2 or greater. It belongs to the genetic predisposition and more consumption of high-energy foods and decreased requirement for physical activity in modern society. This study was designed to evaluate leptin and ghrelin hormones levels and the gene expression of leptin and ghrelin receptors in obese individuals. Seventy-five obese (45 females and 30 males) and 25 (15 females and 10 male) normal individuals were admitted to the Obesity Research and Therapeutic Unit at Alkindy College of Medicine/ University of Baghdad. All blood samples were pulled from obese and normal weight individuals. Leptin and ghrelin hormones were evaluated by ELISA technique and leptin and ghrelin receptors expressions were estimated by RT-PCR. Leptin level was lower in obese than normal 2.74±0.14 ng/ml, 3.47± 0.38 ng/ml respectively. While ghrelin level was higher in obese than normal (26.3 ± 0.56 ng/ml, 17.3 ± 0.5 ng/ml respectively). There was a significant decrease in leptin receptor Ob-Rb mRNA expression in obese individuals (P<0.05). Obese individuals had more ghrelin receptor GHS-R mRNA expression than normal people. In conclusion, obese patients were found to have higher ghrelin hormone and GHS-R as well as low leptin and Ob-Rb mRNA expressions.

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Section: Resultsmentioning

confidence: 99%

Detection of Leptin and Ghrelin Hormones and the Expression of their Receptors in Iraqi Obese Individuals

Alsaffar,

Jumaa,

Baqer

2024

Iraqi Journal of Science

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Effect of Solute Carrier Family 30 A8 Zinc Transporter (SLC30A8) Gene Polymorphism on Serum Zinc Transporter 8 and Zinc Transporter 8 Autoantibodies Levels in Iraqi Women with Gestational Diabetes Mellitus

Alrikabi,

Hussain

2023

Preprint

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Background The solute carrier family 30 A8 zinc transporter (SLC30A8) plays a vital role in insulin synthesis and secretion. This study aimed to investigate the association of rs13266634 C/T polymorphism in the Solute carrier family 30 member 8 (SLC30A8) gene with GDM risk among Iraqi pregnant women in addition to examine maternal serum zinc transporter 8 (ZnT8) and antibodies to ZnT8 (anti-ZnT8 Abs) levels during pregnancy both in women with or without GDM and their associations with SLC30A8 rs13266634 C/T SNP. Methods A case-control study was carried out from November 2022- May 2023 in Baghdad Teaching Hospital/ Pregnancy Care unit/ Baghdad- Iraq. The study sample included 17–43-year-old women; 52 pregnant women with GDM and 50 non-GDM control pregnant women. GDM was diagnosed by the obstetrician based on HbA1c test and ultrasound. GDM risk was tested in a multivariable model, adjusted for age, gravidity and different pregnancy complications. Blood samples were collected from all participants and were genotyped for the rs13266634 SNP by Taqman Real Time quantitative polymerase chain reaction RT-PCR technique. Results SLC30A8 rs13266634 C/T SNP was associated with an increased risk of GDM. The T allele frequency of SLC30A8 rs13266634 C/T SNP was significantly different between GDM and controls (odds ratio [OR]: 2.4; 95% confidence interval [CI]: 1.3535 – 4.2959; P = 0.002). TT genotype versus CC genotype was significantly associated with higher GDM risk (OR = 48; 95% CI: (2.5235 to 91.7594); P = 0.01), and subjects with CT+TT genotype were more likely to have GDM compared to those with CC genotypes, (OR =3.2; 95% CI: 1.2475 – 8.2995; P = 0.009). Serum ZnT8Ab concentration was significantly increased among the GDM and non-GDM who had CT genotype (OR =6.500; 95% CI: 1.787- 23.644; P = 0.003). Conclusions Our study shows that Iraqi pregnant women who are T allele carriers have a higher risk of GDM unlike C allele carriers. ZnT8Ab is protective from GDM while ZnT8 is an independent risk factor which was significantly higher in the GDM subjects.

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Potential Protective Effect of Anti-zinc Transporter 8 Autoantibodies on Gestational Diabetes Mellitus in Iraqi Population

Alrikabi,

Hussain

2024

Biomedical and Biotechnology Research Journal

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Background: The human zinc transporter 8 (ZNT8), also known as the solute carrier family 30A, member 8 (SLC30A8), is a β-cell specific integral membrane protein, mainly restricted to the membrane of insulin secretory granules. ZnT8 plays a crucial role in both insulin synthesis and secretion as well as the viability of β-cells. The recently identified autoantibodies targeting the β-cell surface epitopes of ZnT8 have been proposed as a new risk factor for gestational diabetes mellitus (GDM). Due to the lack of prior research studies on the role of β-cell surface autoantibodies against ZnT8 in the immunologic etiology of GDM in Iraqi population, this study has been designed to explore the association between the rs13266634 C>T polymorphism in the SLC30A8 gene and the serum levels of ZnT8 as well as Zinc Transporter-8 Autoantibody (ZnT8A) in women diagnosed with GDM. Methods: A total of 50 participants with GDM and 50 controls from the Baghdad Teaching Hospital/Pregnancy Care unit at the Medical City, Baghdad, Iraq, were recruited in this case–control study. Serum levels of ZnT8 and ZnT8A were assayed with enzyme-linked immunosorbent assay, and Taqman real time polymerase chain reaction (RT-PCR) was used for rs13266634 single nucleotide polymorphism (SNP) genotyping. Results: ZnT8A levels were significantly lower in women with GDM than non-GDM women (P ≤ 0.01). A ZnT8A level lower than 20.0 pg/mL was significantly associated with a threefold higher risk for GDM with P ≤ 0.001. ZnT8 levels were significantly higher in the GDM subjects (P ≤ 0.05). The T allele at the rs13266634 C>T SNP significantly conferred a higher risk of GDM (P = 0.002). In view of that, the TT genotype, compared to the CC genotype, showed a significant association with increased GDM risk (P ≤ 0.01). Serum ZnT8A concentrations were significantly low among GDM women who had CT genotype (P = 0.003). Conclusion: The T variant at the SLC30A8 rs13266634 C>T polymorphism appears to play a key role in determining the β-cell autoimmunity by inducing an autoantibody response due to its hyperactivity of zinc transportation.

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Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus

Cited by 3 publications

References 22 publications

Detection of Leptin and Ghrelin Hormones and the Expression of their Receptors in Iraqi Obese Individuals

Detection of Leptin and Ghrelin Hormones and the Expression of their Receptors in Iraqi Obese Individuals

Effect of Solute Carrier Family 30 A8 Zinc Transporter (SLC30A8) Gene Polymorphism on Serum Zinc Transporter 8 and Zinc Transporter 8 Autoantibodies Levels in Iraqi Women with Gestational Diabetes Mellitus

Potential Protective Effect of Anti-zinc Transporter 8 Autoantibodies on Gestational Diabetes Mellitus in Iraqi Population

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