Relationship between malondialdehyde production and arachidonate consumption during NADPH-supported microsomal lipid peroxidation

Jordan, Ronald A.; Schenkman, John B.

doi:10.1016/0006-2952(82)90034-x

Cited by 83 publications

(19 citation statements)

References 24 publications

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“…Liver was homogenized with five volumes of 50 mM Tris-HCl buffer (pH 7.3) containing 0.25 (M) sucrose, 10 mM b-mercaptoethanol, 1 mM phenyl methyl sulfonyl fluoride and 1 mM EDTA using Potter Elvehjem homogenizer and lipid peroxidation was determined by estimating malondialdehyde (MDA) as described by Jordan and Schenkman. 15) Pringle described a new technique to reduce blood loss during liver surgery. Adult Wistar rats were subjected to 1 h of partial liver ischemia and followed by 3 h reperfusion.…”

Section: )mentioning

confidence: 99%

<small>L</small>-Arginine Protects from Pringle Manoeuvere of Ischemia-Reperfusion Induced Liver Injury

Chattopadhyay¹,

Verma²,

Verma³

et al. 2008

Biological & Pharmaceutical Bulletin

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During hepatic resection, the risk of severe intra-operative bleeding is a major risk.1) To prevent massive blood loss, continuous or intermittent vascular clamping of the hepatic artery and portal vein ligation is known as "Pringle manoeuvere" is an efficient method to reduce hemorrhage. 2,3) "Pringle manoeuvere" is a technique which leads to ischemic and reperfusion injury. Ischemia and reperfusion results in complex metabolic, 4) immunological 5) and micro vascular 6) changes, which together may contribute to hepatocellular damage and dysfunction. 7) Nitric oxide (NO) is an important modulator of tissue blood flow, arterial pressure, neurotransmission, and immune cell function.8) There is evidence that implicates NO as a modulator of the adhesive interactions among leukocytes, platelets, and endothelial cells 9) and Larginine at a dose level 100 mg/kg/p.o. daily showed significantly protect from alcoholic injury.10) It has been shown that NO-donating compounds provide significant protection against the micro vascular dysfunction that is normally associated with ischemia and reperfusion (I/R).10) L-Arginine is the precursor of NO in vivo and confirmed sustained NO production via L-arginine administration ameliorated effects of intestinal I/R injury.11) However the effect of L-arginine therapy in the view of NO mediated vasodilatation on hepatic ischemia has not yet been completely elucidated. Therefore, this study was designed to investigate how an altered bioavailability of hepatocytes NO produces vasodilatation in hepatic artery supplementation by L-arginine affects the sinusoidal reperfusion and apoptosis under defined surgical techniques i.e. portal triad clamping (Pringle manoeuvere). MATERIALS AND METHODS ChemicalsCollagenase was purchased from Hymedia (Mumbai, India), peroxides-conjugated goat anti-rabbit secondary antibody was purchased from Bio source international (NY, U.S.A.). Alanine transaminase (ALT), aspartate transaminase (AST) kits were procured from Merck India Ltd. (India) and solvents of analytical grade were purchased from Merck (India).Animals Treatment Eighteen Wistar rats were divided into sham-operated control group (I) (nϭ6), ischemia and reperfusion group (II) were given 0.9% saline (5 ml/kg, p.o.) for 7 d (nϭ6), L-arginine pretreated group (100 mg/kg body weight/daily by oral route for 7 d before induced ischemia reperfusion maneuver) (III) (nϭ6). All rats were treated in accordance with the guideline for the Care and Use of Laboratory Animals (NIH Publication No. 86-23, revised 1985) with the permission of institute ethical committee. The hepatic I/R protocol were performed as described earlier. 12)Procurement of Tissue and Blood A portion of the ischemic and non-ischemic liver lobe was fixed in buffered 10% formalin and Karnovsky's solution for histopathology and transmission electron microscopy (TEM) studies. Blood samples were obtained from the right ventricle via a left anterior thoracotomy at the time of sacrifice. The blood from different groups rats were collected in steri...

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Section: )mentioning

confidence: 99%

<small>L</small>-Arginine Protects from Pringle Manoeuvere of Ischemia-Reperfusion Induced Liver Injury

Chattopadhyay¹,

Verma²,

Verma³

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Lipid peroxidation (LPO) rate was measured according to the method of Jordan and Schenkman, (1982). Liver samples were homogenized in 0.1 M phosphate buffer saline and then centrifuged at 5000 × g at 4°C for 5 min.…”

Section: Determination Of Lipid Peroxidation Rate In Livermentioning

confidence: 99%

In vitroandin vivoantitumor activity of a methanol extract ofDregea volubilisleaves with its antioxidant effect

Hossain¹,

Chakroborty

Milan

et al. 2012

Pharmaceutical Biology

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“…Hepatic LPO was measured as method described (7) . To 100 µl separated microsomes in 0.1(M) phosphate buffer saline, 1 mL of 28% trichloroacetic acid was added and centrifuged at x 2000 g at 4oC for 20 minutes.…”

Section: Determination Of Lipid Peroxides (Lpo)mentioning

confidence: 99%

Hepatic hyperplasia and damages induces by zearalenone Fusarium mycotoxins in BALB/c mice

Chatopadhyay

Pandey

Chaurasia

et al. 2012

Arq. Gastroenterol.

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-Context -Zearalenone is a mycoestrogen and considered a mycotoxin. Objective -To establish whether zearalenone produced hepatotoxicity via oral administration. Methods -Zearalenone was orally administered at a dose of 50 μg, 100 μg and 200 μg ZEN/ body weight/daily, respectively, for 14 days to three groups of BALB/c mice. Diagnostic modalities used to evaluate hepatic damage and impaired hepatic function pre-and post zearalenone administration included hepatic marker enzyme activity, pentobarbital sleeping time, cytochrome P-450 activities and histopathologic evaluation of liver. Results -Significant histopathologic changes viz. sinusoidal congestion, cytoplasmic vacuolization, hepatocellular necrosis and neutrophil infiltration were observed after evaluating of liver section from each group after accumulated zearalenone exposure. Further, zearalenone exposure increased activities of alanine transaminase, aspartate transaminase and lipid peroxides whereas activities of tissue glutathione and cytochrome P 450 were decreased as compared to control mice. Zearalenone also increased the sleeping time and decreased sleeping latency after pentobarbital through intraperitoneal route as compared to control mice which indicates that the impairment of hepatic metabolizing enzymes by zearalenone. Conclusion -Zearalenone is a potential hepatotoxin by oral route. Headings -Zearalenone. Hyperplasia. Liver, pathology. Mice.

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Relationship between malondialdehyde production and arachidonate consumption during NADPH-supported microsomal lipid peroxidation

Cited by 83 publications

References 24 publications

<small>L</small>-Arginine Protects from Pringle Manoeuvere of Ischemia-Reperfusion Induced Liver Injury

<small>L</small>-Arginine Protects from Pringle Manoeuvere of Ischemia-Reperfusion Induced Liver Injury

In vitroandin vivoantitumor activity of a methanol extract ofDregea volubilisleaves with its antioxidant effect

Hepatic hyperplasia and damages induces by zearalenone Fusarium mycotoxins in BALB/c mice

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