Relationship between infectivity and OspC expression in Lyme disease Borrelia

Masuzawa, Toshiyuki

doi:10.1016/0378-1097(94)90212-7

Cited by 21 publications

(28 citation statements)

References 14 publications

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“…The reason for this remains unclear; however, it is possible to hypothesize that these noninfectious phenotypes causes a lack of ability to evade the host immune system. In addition to the above, it was previously reported that highpassage strains lost their infectivity in the mouse [25,31,34]. This may be explained by missing plasmids involved in the borrelial infectivity in mammals [34].…”

Section: Discussionmentioning

confidence: 84%

Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a Borrelia valaisiana–related sp. Isolated in Japan is Distinguishable from European B. valaisiana

et al. 2013

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ABSTRACT. Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently, another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequence typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the Borrelia sp. was maintained in the Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and the vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of B. yangtze (=DSM 24625, JCM 17189).

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Section: Discussionmentioning

confidence: 84%

Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a Borrelia valaisiana–related sp. Isolated in Japan is Distinguishable from European B. valaisiana

et al. 2013

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“…Comparisons of B. burgdorferi isolates cultured in vitro have helped to identify B. burgdorferi genes that may be associated with infectivity. Noninfectious B. burgdorferi isolates have been shown to lack, or have reduced expression of or mutations in, ospB, ospD, ospC, or vls, among other genes, and the loss of several plasmids, including linear plasmid (lp) 25, lp28.4, and circular plasmid 9 (5,16,19,20,24,25,28,(31)(32)(33)(34). A direct cause and effect relationship has not yet been established for any single gene or plasmid: for example, some noninfectious B. burgdorferi B31 clones express ospD and other infectious clones do not (15,(19)(20)(21).…”

mentioning

confidence: 99%

Dissociation of Infectivity and Pathogenicity in Borrelia burgdorferi

et al. 2001

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Clonal Borrelia burgdorferi N40 (cN40) passaged 75 times in vitro (N40-75) infects mice but does not cause disease. N40-75 passaged 45 times further in vitro (N40-120) was no longer infectious and lacked genes encoded on linear plasmids 38 and 28-1, among other differences. These data suggest that B. burgdorferi cN40, N40-75, and N40-120 have distinct phenotypes that can be used to dissect the genetic elements responsible for pathogenicity and infectivity.

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“…However, virulence factors of B. burgdorferi responsible for development of the signs of the Lyme disease might be different from the factors responsible for infecting the host tissues such as adhesions of this bacterium. Masuzawa et al (6), found a relationship between infectivity of low passage B. burgdorferi strains, 297, HP1, 12-92 and OspC expression. Non-pathogenic, B. burgdorferi infection rate in mice was very low and showed reduced expression of OspC after growth in BSK medium in contrast to low passage spirochetes.…”

Section: Discussionmentioning

confidence: 98%

“…OspA is synthesized in Barbour, Stoenner, Kelly's medium (BSK) culture of B. burgdorferi except a naturally occurring mutant that lacks this protein. There is a report regarding the relationship between the infectivity of B. burgdorferi 297 and abundant expression of OspC in low BSK passage cultures (6). It has been found that ospC and revA genes encode products that functioned in establishing mammalian infection and in fibronectin-binding, respectively (7).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Adhesion and OspC Protein Synthesis of the Lyme Disease Spirochete Borrelia Burgdorferi Cultivated in a Host-Derived Tissue Co-Culture System

Şen

Sigal

2013

Balkan Med J

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Background: The adhesion process of Borrelia burgdorferi to susceptible host cell has not yet been completely understood regarding the function of OspA, OspB and OspC proteins and a conflict exists in the infection process.Aims: The adhesion rates of pathogenic (low BSK medium passaged or susceptible rat joint tissue co-cultivated ) or non-pathogenic Borrelia burgdorferi (high BSK medium passaged) isolate (FNJ) to human umbilical vein endothelial cells (HUVEC) cultured on coverslips and the synthesis of OspA and OspC proteins were investigated to analyze the infection process of this bacterium.Study Design: In-vitro study.Methods: Spirochetes were cultured in BSK medium or in a LEW/N rat tibiotarsal joint tissue feeder layer supported co-culture system using ESG coculture medium and labelled with 3H-adenine for 48 hours. SDS-PAGE, Western Blotting, Immunogold A labeling as well as radiolabeling experiments were used to compare pathogenic or non pathogenic spirochetes during the adhesion process.Results: Tissue co-cultured B. burgdorferi adhered about ten times faster than BSK-grown spirochetes. Trypsin inhibited attachment to HUVEC and coculture of trypsinized spirochetes with tissues reversed the inhibition. Also, the synthesis of OspC protein by spirochetes was increased in abundance after tissue co-cultures, as determined by SDS-PAGE and by electron microscopy analysis of protein A-immunogold staining by anti-OspC antibodies. OspA protein was synthesized in similar quantities in all Borrelia cultures analyzed by the same techniques.Conclusion: Low BSK passaged or tissue co-cultured pathogenic Lyme disease spirochetes adhere to HUVEC faster than non-pathogenic high BSK passaged forms of this bacterium. Spirochetes synthesized OspC protein during host tissue-associated growth. However, we did not observe a reduction of OspA synthesis during host tissue co-cultivation in vitro.

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Relationship between infectivity and OspC expression in Lyme disease Borrelia

Cited by 21 publications

References 14 publications

Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a <i>Borrelia valaisiana</i>–related sp. Isolated in Japan is Distinguishable from European <i>B. valaisiana</i>

Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a <i>Borrelia valaisiana</i>–related sp. Isolated in Japan is Distinguishable from European <i>B. valaisiana</i>

Dissociation of Infectivity and Pathogenicity in Borrelia burgdorferi

Enhanced Adhesion and OspC Protein Synthesis of the Lyme Disease Spirochete Borrelia Burgdorferi Cultivated in a Host-Derived Tissue Co-Culture System

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