Relationship between immune system and gram-negative bacteria: Monocyte chemotaxis induced by supernatants from human peripheral blood OKT8+ lymphocytes stimulated with smooth and rough Salmonella strains

Antonaci, Salvatore; Jirillo, Emilio

doi:10.1016/0008-8749(85)90313-2

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…gingivalis- stimulated epithelial cells could not trigger effective migration of PMNs [ 21 ]. However, a previous study demonstrates that the supernatants from cultured mononuclear cells infected by different Gram-negative bacteria could trigger chemotaxis of monocytes [ 22 ], although the extent may be different depending on the differential species investigated. This observation suggests that the chemotactic effects may depend on the nature of the stimulating species.…”

Section: Discussionmentioning

confidence: 99%

Human Oral Keratinocytes Challenged by Streptococcus sanguinis and Porphyromonas gingivalis Differentially Affect the Chemotactic Activity of THP-1 Monocytes

Seneviratne

Jin

2022

International Journal of Microbiology

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Periodontal diseases are initiated by the shift from microbe-host symbiosis to dysbiosis, and the disrupted host response predominantly contributes to tissue destruction. This study investigated whether and to what extent human oral keratinocytes (HOKs) challenged by a periodontal commensal or pathogen could differentially affect the chemotactic activity of THP-1 monocytes. A selected periodontal commensal (Streptococcus sanguinis ATCC 10556) and a pathogen (Porphyromonas gingivalis ATCC 33277) were cultured and inoculated, respectively, into the lower chamber of Transwell® Permeable Supports with HOKs and incubated for 2 h or 18 h at 37°C under appropriate cell growth conditions. HOKs alone served as the control for the transwell migration assay. Well-stained THP-1 monocytes were seeded in the top chamber of the device, incubated for 2 h and then collected from the lower well for quantitation of the migrated fluorescence-labeled cells by the FACSCalibur™ flow cytometer. The statistical significance was determined using one-way ANOVA. The HOKs challenged by S. sanguinis attracted a significantly higher number of THP-1 cell migration as compared with the control after 2 h or 18 h interaction ( p < 0.01 ). By contrast, P. gingivalis-treated HOKs exhibited a markedly reduced chemotactic effect on THP-1 cells ( p < 0.01 , 2 h; p < 0.05 , 18 h). There was no significant difference in THP-1 cell migration among the groups with either S. sanguinis or P. gingivalis alone. The current findings on P. gingivalis-HOKs interactions with resultant paralysis of THP-1 cell chemotaxis provide further evidence that the keystone periodontopathogen P. gingivalis can evade innate defense and contribute to periodontal pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Human Oral Keratinocytes Challenged by Streptococcus sanguinis and Porphyromonas gingivalis Differentially Affect the Chemotactic Activity of THP-1 Monocytes

Seneviratne

Jin

2022

International Journal of Microbiology

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“…The role of lymphocytes that undergo bacterial binding could be the transport of microorganisms to distant organs, such as spleen, where phagocytosis can occur (15). On the other hand, in local inflammatory sites, such as lymph nodes and lymphoid tissues, the direct impact of bacteria with lymphocytes gives rise to the production of lymphokines (LK), such as Leukocyte Inhibitory Factor (LIF) and Lymphocyte-Derived Chemotactic Factor (LDCF) (23,24). These two LKs have an important role in the attraction and accumulation of monocytes and PMN in the sites of inflammation, thus protecting the host against invading organisms.…”

Section: Discussionmentioning

confidence: 99%

Effects of substance p on the spontaneous binding of salmonella minnesota r345 (rb) to human peripheral blood lymphocytes

Simone

Misefari

Antonaci

et al. 1989

Clinical Laboratory Analysis

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The effects of substance P (SP) on Salmonella minnesota R345 (Rb) binding to human peripheral blood lymphocytes (PBL) were evaluated. Two parameters of bacterial cytoadherence were considered, namely the binding lymphocytes (BL) and the number of bound-bacteria/lymphocyte (BB). The results showed that SP inhibits both BL and BB in a significant manner. Furthermore, distribution of Salmonella binding to CD4+ and CD8+ lymphocytes was studied following SP pretreatment of lymphoid cells. This neuropeptide is able to hamper the bacterial cytoadherence to both T-cell subpopulations and, in particular, the inhibitory effect on the T-suppressor/cytotoxic subset was more pronounced. These findings are discussed in terms of SP intervention in the mechanism of host protection against invading microorganisms.

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Stimulation of Immunity by Probiotics

Famularo¹,

Moretti²,

Marcellini³

et al. 1997

Probiotics 2

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Relationship between immune system and gram-negative bacteria: Monocyte chemotaxis induced by supernatants from human peripheral blood OKT8+ lymphocytes stimulated with smooth and rough Salmonella strains

Cited by 4 publications

References 15 publications

Human Oral Keratinocytes Challenged by Streptococcus sanguinis and Porphyromonas gingivalis Differentially Affect the Chemotactic Activity of THP-1 Monocytes

Human Oral Keratinocytes Challenged by Streptococcus sanguinis and Porphyromonas gingivalis Differentially Affect the Chemotactic Activity of THP-1 Monocytes

Effects of substance p on the spontaneous binding of salmonella minnesota r345 (rb) to human peripheral blood lymphocytes

Stimulation of Immunity by Probiotics

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