Relationship between Human Papillomavirus (HPV) Genotyping and Genital Neoplasia in HIV-Positive Patients of Santos City, São Paulo, Brazil

Abstract

Among HIV-positive women, infection with multiple human papillomavirus (HPV) types is known to be more frequent and persistent, as well as a greater prevalence of high-grade genital lesions. This study aims to characterize, for the first time in Brazil, HPV presence and types among HIV-positive women of a high-risk population for genital intraepithelial neoplasia (GIN) development. A non-anonymous, voluntary, cross-sectional epidemiological survey was conducted, from June 1996 to April 1997, among 141 HIV-positive women followed at the Center of Reference in AIDS (CRAIDS). They were submitted to gynaecological examination, cytological screening and biopsies whenever necessary, for GIN and HPV DNA detection through polymerase chain reaction (PCR) technique. Positive HPV DNA were found in 80.8% patients. Two or more HPV types were detected in 45% of the samples. The most frequent HPV types detected were 16, 18 (30.5%); 61, 53 (24.4%), and non-identified types (18.7%). According to the oncogenic potential, 34.8% were considered of high-risk types. Among these HIV-positive patients, a great variety of HPV types, including high-risk types, was found in anogenital environment, as well as among young women, a great prevalence of high-grade genital lesions. Thus, it should strengthen the need for a periodical careful gynaecological examination among those women.

High risk HPV types in southern Iranian patients with cervical cancer

“…In total, 87.1% of studied samples were positive for the presence of HPV DNA, which was comparable with the results of some previous studies in Danish, Belgian, Brazilian and Chinese patients. [17][18][19][20] Genotyping for HPV-16 and HPV-18 revealed that 26.7% of HPV positive samples were HPV-16 DNA positive, however, none of them were positive for HPV-18 DNA. The very low rate of HPV-16 infection in cervical cancer is contrary to the previous reports of high infection rate of this high risk type in different populations ( Table 2 ).…”

“…17,19,21 However, it is comparable to the results of some other studies. 13,18,22 The study of Castellsague in Mozambique 13 has shown that there may be differences in the dominant HPV types in different populations and regions. In this regard, the report of higher HPV-16 titers in carriers of a specific HLA-DRB1-DQB1 haplotype is noteworthy.…”

“…This is contrary to the high rate of HPV-18 detection in Australia, Senegal and Hong-Kong. 18,20,21 HPV-18 is mostly found in adenocarcinoma of cervix and has a low rate in squamous cell carcinoma of cervix. 27,28 Since only 8 (7.9%) of 101 samples in this study were adenocarcinomas, it is logical to assume that not having HPV-18 genotype is due to the low adenocarcinoma case numbers.…”

Human papillomavirus prevalence among women with cervical intraepithelial neoplasia III and invasive cervical cancer from Goiânia, Brazil

“…In Brazil, a country with almost continental proportions, studies of HPV prevalence in cervical cancer from four of its five regions are available. All these published studies showed that HPV 16 is the predominant type in cities of the Northern, Northeast, Southeast and Southern regions, although there are regional variations with regard to other types (Cavalcanti et al 1994, Eluf-Neto et al 1994, Bosch et al 1995, Gonçalves et al 1999, Noronha et al 1999, Lorenzato et al 2000.…”

“…Studies of Brazilian women showed that HPV DNA prevalence in invasive cervical cancer ranged from 55.2% when using non-isotopic in-situ hybridization to 91% when using PCR-based assays (Cavalcanti et al 1994, ElufNeto et al 1994, Bosch et al 1995, Gonçalves et al 1999, Noronha et al 1999, Lorenzato et al 2000.…”