Relationship between HLA I surface expression and different cytopathic effects produced after herpes simplex virus infection in vitro

Walev, Iwan; Kunkel, John; Schwaeble, Wilhelm; Weise, K.; Falke, D.

doi:10.1007/bf01309703

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Polycaryocytosis after infection of vaginal epithelium is worth mentioning; recently, we have shown that there is a loss of MHC antigens on cells exhibiting fusion but not cell rounding [15]. Studies are now in progress to determine whether this difference can also be detected in vivo.…”

Section: Discussionmentioning

confidence: 96%

Pathogenesis of HSV-1/2 induced vaginitis/vulvitis of the mouse: dependence of lesions on genetic properties of the virus and analysis of pathohistology

Podlech

Weise

Müntefering

et al. 1993

Archives of Virology

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A scoring system for herpes simplex virus (HSV) induced vaginitis/vulvitis in Balb/c mice was delineated from vaginal infections. Four degrees of vaginitis/vulvitis could be distinguished after infection with suitable strains of HSV despite nearly identical replication rates. The time course of replication, inflammation and pathohistology was compared further. Grade 0 was defined by lack of symptoms despite presence of strong replication, which was detectable at days 3-6. Focal necrotic lesions of the epithelial layer were present containing HSV-specific antigens. DNA could be detected by hybridization only in the outer zone of these areas. At day 6 these zones began to be re-epithelialized. In the vaginal lumen abundant detached epithelial cells and granulocytes were already present by day 2. Grade 1 was macroscopically characterized by a slight inflammation commencing on days 5-6. Replication and antigens in the epithelium were found on days 2-6. HSV-antigens were only detected above the basal membrane, and some infiltration with granulocytes and lymphocytes was observed below the basal membrane at day 4. Grade 2 showed strong redness and inflammation as well as hyperemia. Cellular infiltrates were present in the large antigen containing epithelial lesions and below the basal membrane. From day 4 on, neurons were HSV-antigen and DNA positive and macrophages in the stroma contained antigen. The vulva was also shown to be involved. Grade 3 exhibited prolonged severe hyperemia, and destruction of the epithelium and the stroma with necrosis and infiltration, especially of the vulva. This grading system was shown to depend on certain unknown genetic properties of HSV-strains. Neither thymidine-kinase activity, replication in macrophages, fusion activity of strains nor presence or absence of the Hpa I P-fragment were shown to be of importance for severity of vaginitis/vulvitis. Vaginitis/vulvitis was shown to be an all or none response to HSV independent of the rate of replication. The set of virus genes responsible for neuroinvasiveness after vaginal or i.p. inoculation was found to be different. The time course of replication (mainly days 3-6) and inflammation (days 5-10) indicates that inflammation seems to be a secondary immunological phenomenon induced later by the replication phase of HSV. Our system could be useful for separately testing drugs with antiviral and anti-inflammatory properties.

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Section: Discussionmentioning

confidence: 96%

Pathogenesis of HSV-1/2 induced vaginitis/vulvitis of the mouse: dependence of lesions on genetic properties of the virus and analysis of pathohistology

Podlech

Weise

Müntefering

et al. 1993

Archives of Virology

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“…s @ w 1 S . v MHC hyperexpression induced by ectopic changes in the immunosuppressive properties of the intraocupable of overriding a possibly subtle inter-lar microenvironment might be responsible for the protection n expression (58,59) by the virus. of the second eyes in transgenic mice (2).…”

Section: Discussionmentioning

confidence: 99%

Ectopic expression of gamma interferon in the eye protects transgenic mice from intraocular herpes simplex virus type 1 infections

Geiger

Howes

Sarvetnick

1994

J Virol

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Transgenic (rhoy) mice provide a model for studying the influence of gamma interferon (IFN-y) produced in the eye on ocular and cerebral viral infection. To establish this model, we injected BALB/c-and C57BL/6-derived transgenic and nontransgenic mice of different ages intravitreally with herpes simplex virus type 1 (HSV-1) strain F. Eye and brain tissues of these mice were assessed for pathological and immunocytochemical changes. HSV-1 infection induced severe retinitis of the injected eyes and infection of the brain in all mice. In transgenic mice inoculated with HSV-1, the left, nontreated eyes were protected from retinitis,

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Reduced CD21 (CR2) and CD54 (ICAM-1) expression in MT2 cells with HIV-1 or HIV-2 strains

et al. 1995

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Relationship between HLA I surface expression and different cytopathic effects produced after herpes simplex virus infection in vitro

Cited by 4 publications

References 18 publications

Pathogenesis of HSV-1/2 induced vaginitis/vulvitis of the mouse: dependence of lesions on genetic properties of the virus and analysis of pathohistology

Pathogenesis of HSV-1/2 induced vaginitis/vulvitis of the mouse: dependence of lesions on genetic properties of the virus and analysis of pathohistology

Ectopic expression of gamma interferon in the eye protects transgenic mice from intraocular herpes simplex virus type 1 infections

Reduced CD21 (CR2) and CD54 (ICAM-1) expression in MT2 cells with HIV-1 or HIV-2 strains

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