Reduced CD21 (CR2) and CD54 (ICAM-1) expression in MT2 cells with HIV-1 or HIV-2 strains

Larcher, Clara; Julen, Nathalie; Prodinger, W. M.; Tötsch, Martin; Gürtler, Lutz; Dierich, Manfred P.

doi:10.1016/0165-2478(95)00015-w

Cited by 6 publications

(1 citation statement)

References 30 publications

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“…48 After infection of the human T lymphoblastoid cell line MT2 with HIV-1 and HIV-2 isolates expression of CR2 on the cell surface is down-regulated. 19,20 A similar decrease of CR2 has also been observed in vivo with the percent of CD4 + cells expressing CR2 being significantly decreased in HIV-infected patients compared to uninfected control donors. 49 The diminished CR2 synthesis in vivo may be assigned to an increased immune complex formation and complement activation, as demonstrated by higher levels of the complement activation fragments C3d, C4d and Ba of the classical and alternative pathway in HIV-infected individuals.…”

Section: The Complement Receptor Type 2 (Cr2)supporting

confidence: 49%

True

1999

Leukemia

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Section: The Complement Receptor Type 2 (Cr2)supporting

confidence: 49%

True

1999

Leukemia

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Neutralization of HIV Type 1 by Alloimmune Sera Derived from Polytransfused Patients

Spruth

Stoiber²,

Kacani³

et al. 1999

AIDS Research and Human Retroviruses

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Antibodies (Abs) against HLA and other cell surface molecules, which HIV-1 acquires during the budding process at the host cell surface, neutralize HIV-1 in vitro. Macaques were protected against infection by SIV grown in human cells after xenoimmunization with human MHC molecules. Besides the immune responses arising against xenogeneic antigens, the highly polymorphic character of the HLA antigens enables the induction of alloresponses after exposure to allogeneic HLA molecules. Since polytransfused (PT) patients develop alloresponses, including humoral anti-HLA responses, we assumed that sera derived from PT patients may neutralize HIV-1. In a model system two PT sera out of a panel of 12 PT and 6 normal control sera neutralized HIV IIIB in vitro. Neutralizing activity of the PT sera was comparable to the efficacy of anti-HIV sera. The neutralizing capacity coincided with strong IgG reactivity against (HIV-infected) cell lines, which were used for virus production, and recognition of cell-free viral particles. Active human complement enhanced HIV neutralization mediated by the sera. Our results suggest an IgG-mediated neutralization based on recognition of allogeneic HLA molecules expressed on the viral surface. A vaccination strategy based on alloimmunization appears conceivable and requires further investigation.

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Altered Expression of CD4, CD54, CD62L, and CCR5 in Primary Lymphocytes Productively Infected with the Human Immunodeficiency Virus

Marodon

Landau²,

Posnett³

1999

AIDS Research and Human Retroviruses

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Infection of T cells with HIV-1 induces loss of CD4 and HLA class I from the cell surface. In the present article we have investigated whether changes in expression of other cell surface molecules could be related to HIV infection. To detect HIV-infected cells at the single-cell level, peripheral blood lymphocytes were infected in vitro with HIV-HSA, a reporter virus encoding the murine heat-stable antigen. Expression of HSA on activated primary lymphocytes was an efficient indicator of productive infection. Expression of the majority of the cell surface proteins studied was unaffected by HIV infection (HLA class I, II, CD11a, CD18, CD25, CD27, CD28, CD29, CD30, CD31, CD38, CD44, CD45R0, CD49d, CD57, CD94, CD95, and CXCR4). However, phenotypic changes specific to the productively infected cells were detected. Expression of the CD4 molecule was progressively lost and this was closely associated with loss of CD62L expression, a molecule involved in T cell homing into the lymph nodes. By contrast, T cells productively infected with this T-tropic reporter virus were enriched for CD54, and for CCR5, the main coreceptor for M-tropic viruses. Given the roles of CD62L, CD54, and CCR5 in lymphocyte trafficking, these results suggest that cells productively infected with HIV might have altered homing patterns in vivo.

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Reduced CD21 (CR2) and CD54 (ICAM-1) expression in MT2 cells with HIV-1 or HIV-2 strains

Cited by 6 publications

References 30 publications

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Neutralization of HIV Type 1 by Alloimmune Sera Derived from Polytransfused Patients

Altered Expression of CD4, CD54, CD62L, and CCR5 in Primary Lymphocytes Productively Infected with the Human Immunodeficiency Virus

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