Relationship between gut microbiota and circulating metabolites in population-based cohorts

Abstract

Gut microbiota has been implicated in major diseases affecting the human population and has also been linked to triglycerides and high-density lipoprotein levels in the circulation. As recent development in metabolomics allows classifying the lipoprotein particles into more details, we aimed to examine the impact of gut microbiota on circulating metabolites measured by Nuclear Magnetic Resonance ( 1 H-NMR) technology in 2,309 individuals from the Rotterdam Study and the LifeLines-DEEP cohort in whom gut microbiota was profiled using 16S rRNA gene sequencing. The relationship between gut microbiota and metabolites was assessed by linear regression analysis while adjusting for age, sex, body-mass index, technical covariates, medication use, and multiple testing. Our analysis revealed association of 32 microbial families and genera with very-low-density and high-density subfractions, serum lipid measures, glycolysis-related metabolites, amino acids, and acute phase reaction markers. These observations provide novel insights into the role of microbiota in host metabolism and support the potential of gut microbiota as a target for therapeutic and preventive interventions.

Maternal Microbiota, Cortisol Concentration, and Post-Partum Weight Recovery Are Dependent on Mode of Delivery

“…Furthermore, both VAG delivery and the cortisol concentration are known to be associated with health-related genera, including members of the Christensenellaceae [ 77 ], Lachnospiraceae , and Ruminococcaceae families [ 51 ]. In fact, Vojinovic et al found these groups to be correlated and involved in several metabolic pathways, including SCFA production and bile acid metabolism [ 78 ]. In particular, the Christensenellaceae family has been observed to be inversely associated with the BMI [ 79 , 80 ] of lean individuals [ 81 ], as well as with a healthy condition, in studies investigating inflammatory bowel disease (IBD) [ 82 ] and other inflammatory gut-related disorders [ 83 , 84 ].…”

Leptin alters energy intake and fat mass but not energy expenditure in lean subjects

“…The NMR metabolomics (Nightingale Health Ltd., Helsinki, Finland) method has also been used and described in refs. [68][69][70][71][72][73] . The effects of these experimental aspects on the metabolic biomarker concentrations are best reflected in the coefficients of variation (CV) for the measurements, and representative CVs have been published previously in the supplement of refs.…”

Integration of epidemiologic, pharmacologic, genetic and gut microbiome data in a drug–metabolite atlas

“…In total, 92 bacterial taxa abundancesassessed by tag sequencing of the 16 S rRNA gene 58 and Shannon's diversity index (alpha-diversity)-were reported to be significantly different between PPI users and non-users (211 PPI users and 1,594 non-users, false discovery rate < 0.05). Forty-five of the 92 bacterial taxa abundance and alpha-diversity factors were also tested for association with metabolites as measured by Nightingale Health in our previous study 80 . In brief, this included 2,309 individuals not prescribed antibiotics from Rotterdam Study (n = 1,390) and LifeLines DEEP (n = 915) 47,58 .…”