Relationship Between Glycerolipids and Photosynthetic Components During Recovery of Thylakoid Membranes From Nitrogen Starvation-Induced Attenuation in Synechocystis sp. PCC 6803

Kobayashi, Koichi; Osawa, Yuka; Yoshihara, Akiko; Shimojima, Mie; Awai, Kazuo

doi:10.3389/fpls.2020.00432

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…In the membranes, lipids are required for photosynthetic thermostability during elevation of temperature [ 139 , 140 ]. The right temperature is necessary to protect and stabilize the photosystems, allowing the plant to maintain a functional and efficient photosynthetic machinery [ 141 ]. Temperature elevation reduces the membrane thickness by hydrophobic interaction in the membrane [ 76 ].…”

Section: Fatty Acids and Lipids Composition Under Adverse Conditionsmentioning

confidence: 99%

Lipids Composition in Plant Membranes

Reszczyńska

Hanaka

2020

Cell Biochem Biophys

120

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The paper focuses on the selected plant lipid issues. Classification, nomenclature, and abundance of fatty acids was discussed. Then, classification, composition, role, and organization of lipids were displayed. The involvement of lipids in xantophyll cycle and glycerolipids synthesis (as the most abundant of all lipid classes) were also discussed. Moreover, in order to better understand the biomembranes remodeling, the model (artificial) membranes, mimicking the naturally occurring membranes are employed and the survey on their composition and application in different kind of research was performed. High level of lipids remodeling in the plant membranes under different environmental conditions, e.g., nutrient deficiency, temperature stress, salinity or drought was proved. The key advantage of lipid research was the conclusion that lipids could serve as the markers of plant physiological condition and the detailed knowledge on lipids chemistry will allow to modify their composition for industrial needs.

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Section: Fatty Acids and Lipids Composition Under Adverse Conditionsmentioning

confidence: 99%

Lipids Composition in Plant Membranes

Reszczyńska

Hanaka

2020

Cell Biochem Biophys

120

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“…However, the relative proportion of these most abundant DGDG species did not change with the light intensity. The MGDG/DGDG ratio was 2.64 and 2.15 under LL and HL, respectively; provided that this ratio is independent of the chlorophyll content or cell number, it seems evident that high light conditions imply a higher DGDG content, a fact that is likely related to an increase in the PSI/PSII ratio and enhanced thermotolerance [7,57]. Swelling of the thylakoid lumen under illumination in cyanobacteria has been reported [58], and this feature implies a reduction of the membrane curvature, a fact that is likely to be associated with the increase in the content of the bilayer-forming DGDG species.…”

Section: Discussionmentioning

confidence: 99%

Differential Membrane Lipid Profiles and Vibrational Spectra of Three Edaphic Algae and One Cyanobacterium

Montero

Velasco

Miñón

et al. 2021

IJMS

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The membrane glycerolipids of four phototrophs that were isolated from an edaphic assemblage were determined by UPLC–MS after cultivation in a laboratory growth chamber. Identification was carried out by 18S and 16S rDNA sequencing. The algal species were Klebsormidium flaccidum (Charophyta), Oocystis sp. (Chlorophyta), and Haslea spicula (Bacillariophyta), and the cyanobacterium was Microcoleus vaginatus (Cyanobacteria). The glycerolipid profile of Oocystis sp. was dominated by monogalactosyldiacylglycerol (MGDG) species, with MGDG(18:3/16:4) accounting for 68.6%, whereas MGDG(18:3/16:3) was the most abundant glycerolipid in K. flaccidum (50.1%). A ratio of digalactosyldiacylglycerol (DGDG) species to MGDG species (DGDG/MGDG) was shown to be higher in K. flaccidum (0.26) than in Oocystis sp. (0.14). This ratio increased under high light (HL) as compared to low light (LL) in all the organisms, with its highest value being shown in cyanobacterium (0.38–0.58, LL−HL). High contents of eicosapentaenoic acid (EPA, C20:5) and hexadecenoic acid were observed in the glycerolipids of H. spicula. Similar Fourier transform infrared (FTIR) and Raman spectra were found for K. flaccidum and Oocystis sp. Specific bands at 1629.06 and 1582.78 cm−1 were shown by M. vaginatus in the Raman spectra. Conversely, specific bands in the FTIR spectrum were observed for H. spicula at 1143 and 1744 cm−1. The results of this study point out differences in the membrane lipid composition between species, which likely reflects their different morphology and evolutionary patterns.

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“…Thus, membrane lipid remodeling responding to CO 2 may have a different mechanism from that observed under phosphate‐limited conditions. Phosphate or nitrogen limitation blocks cell division of Synechocystis (Kobayashi et al ., 2020). During such conditions, the turnover of membrane lipids might be important rather than de novo synthesis of lipids.…”

Section: Discussionmentioning

confidence: 99%

Membrane lipid remodeling is required for photosystem II function under low CO₂

et al. 2020

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SummaryMembrane lipid remodeling in plants and microalgae has a crucial role in their survival under nutrient‐deficient conditions. Aquatic microalgae have low access to CO2, an essential carbon source for photosynthetic assimilates; however, 70–90 mol% of their membrane lipids are sugar‐derived lipids (glycolipids) such as monogalactosyldiacylglycerol (MGDG). In this study, we discovered a new system of membrane lipid remodeling responding to CO2 in Synechocystis sp. PCC 6803, a unicellular, freshwater cyanobacterium. As compared with higher CO2 (HC; 1% CO2), under ambient air (lower CO2: LC), phosphatidylglycerol (PG) content was increased at the expense of MGDG content. To explore the biological significance of this alteration in content, we generated a transformant of Synechocystis sp. PCC 6803 overexpressing sll0545 gene encoding a putative phosphatidic acid phosphate (oxPAP), which produces diacylglycerol that is used for the synthesis of glycolipids, and examined the effect on membrane lipid remodeling and phototrophic growth responding to LC. Photosystem II (PSII) activity and growth rate were inhibited under LC in oxPAP cells. PG content was substantially reduced, and MGDG and sulfoquinovosyldiacylglycerol contents were increased in oxPAP cells as compared with control cells. These phenotypes in oxPAP cells were recovered under the HC condition or PG supplementation. Increased PG content may be required for proper functioning of PSII under LC conditions.

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Relationship Between Glycerolipids and Photosynthetic Components During Recovery of Thylakoid Membranes From Nitrogen Starvation-Induced Attenuation in Synechocystis sp. PCC 6803

Cited by 8 publications

References 40 publications

Lipids Composition in Plant Membranes

Lipids Composition in Plant Membranes

Differential Membrane Lipid Profiles and Vibrational Spectra of Three Edaphic Algae and One Cyanobacterium

Membrane lipid remodeling is required for photosystem II function under low CO₂

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Relationship Between Glycerolipids and Photosynthetic Components During Recovery of Thylakoid Membranes From Nitrogen Starvation-Induced Attenuation in Synechocystis sp. PCC 6803

Cited by 8 publications

References 40 publications

Lipids Composition in Plant Membranes

Lipids Composition in Plant Membranes

Differential Membrane Lipid Profiles and Vibrational Spectra of Three Edaphic Algae and One Cyanobacterium

Membrane lipid remodeling is required for photosystem II function under low CO2

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Membrane lipid remodeling is required for photosystem II function under low CO₂