Relationship between fruit maturation stage and physiological quality of physic nut (Jatropha curcas L.) seeds

Silva, Laércio Junio da; Dias, Denise Cunha Fernandes dos Santos; Milagres, Carla do Carmo; Dias, Luíz Antônio dos Santos

doi:10.1590/s1413-70542012000100005

Cited by 19 publications

(13 citation statements)

References 12 publications

(16 reference statements)

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“…Seeds of a selected J. curcas plant in Kamisse, Ethiopia, were collected at six maturation stages (I-VI) and characterized according to the color of epicarp and endocarp [green-white (I), green-brown (II), green-black (III), yellow-black (IV), brown-black (V), dry-black (VI)] [94] at the same time. Three biological replicates were used for each sample, immediately frozen in liquid nitrogen and stored at − 80°C.…”

Section: Plant Materialsmentioning

confidence: 99%

Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas

et al. 2020

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Background: Jatropha curcas, a tropical shrub, is a promising biofuel crop, which produces seeds with high content of oil and protein. To better understand the maturation process of J. curcas seeds and to improve its agronomic performance, a two-step approach was performed in six different maturation stages of seeds: 1) generation of the entire transcriptome of J. curcas seeds using 454-Roche sequencing of a cDNA library, 2) comparison of transcriptional expression levels using a custom Agilent 8x60K oligonucleotide microarray. Results: A total of 793,875 high-quality reads were assembled into 19,382 unique full-length contigs, of which 13,507 could be annotated with Gene Ontology (GO) terms. Microarray data analysis identified 9111 probes (out of 57,842 probes), which were differentially expressed between the six maturation stages. The expression results were validated for 75 selected transcripts based on expression levels, predicted function, pathway, and length. Result from cluster analyses showed that transcripts associated with fatty acid, flavonoid, and phenylpropanoid biosynthesis were over-represented in the early stages, while those of lipid storage were over-represented in the late stages. Expression analyses of different maturation stages of J. curcas seed showed that most changes in transcript abundance occurred between the two last stages, suggesting that the timing of metabolic pathways during seed maturation in J. curcas occurs in late stages. The co-expression results showed that the hubs (CB5-D, CDR1, TT8, DFR, HVA22) with the highest number of edges, associated with fatty acid and flavonoid biosynthesis, are showing a decrease in their expression during seed maturation. Furthermore, seed development and hormone pathways are significantly well connected. Conclusion: The obtained results revealed differentially expressed sequences (DESs) regulating important pathways related to seed maturation, which could contribute to the understanding of the complex regulatory network during seed maturation with the focus on lipid, flavonoid and phenylpropanoid biosynthesis. This study provides detailed information on transcriptional changes during J. curcas seed maturation and provides a starting point for a genomic survey of seed quality traits. The results highlighted specific genes and processes relevant to the molecular mechanisms involved in Jatropha seed maturation. These data can also be utilized regarding other Euphorbiaceae species.

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Section: Plant Materialsmentioning

confidence: 99%

Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas

et al. 2020

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“…Anandalakshmi (2009) recommended harvesting yellow fruits for maximum oil yield. In another study (Silva et al 2012), the oil content of different stages of maturity shows that the seeds from green fruits had less oil compared to the seeds from other fruit categories, which had similar levels of oil. However, uncertainty existed to choose the best maturity stage in the literature.…”

Section: Introductionmentioning

confidence: 90%

Does the Maturity ofJatropha CurcasL. Affect the Quality and Quantity of the Yield of Oil for Biodiesel Production?

Samsuri

Zoveidavianpoor

2013

International Journal of Green Energy

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Biodiesel is a green and popular renewable fuel and unlike mineral diesel, produces fewer toxic emissions. Jatropha curcas Linn is a nonedible fruit that commonly used in biodiesel production. This study evaluated the impacts of Jatropha seeds maturity on quantity and quality of yield oil. Production of biodiesel from Jatropha oil and ethanol using natrium hydroxide as the catalyst by transesterification was performed on half-matured (yellow) and matured (black) Jatropha seeds. Experimental investigations have been carried out to examine properties and performance of Jatropha, and different blends of Jatropha oil-diesel (JOD) in comparison to a petroleum-based diesel fuel. Half-matured stage of Jatropha is shown to produces 2.5% less oil than the matured stage. The quality of the maturity stages quantified in terms of mean square error (MSE), and the matured stage showed 13% better performance in contrast to the half-matured stage. Matured stage JOD blends indicate closer performance to petroleum-based diesel and can be used as a biodiesel without engine modification.

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“…Flowering and fruiting of Jatropha are fairly continuous, which results in the presence of mature and immature fruits at the same time on the same plant. Seeds of a selected J. curcas accession in Kamisse, Ethiopia, Agricultural Research Center Adama, were collected at six maturation stages (I-VI) and characterized according to the color of epicarp and endocarp [green-white (I), green-brown (II), green-black (III), yellow-black (IV), brown-black (V), dry-black (VI)] [20] at the same time. Three biological replicates were used for each sample, immediately frozen in liquid nitrogen and stored at -80 °C.…”

Section: Plant Materialsmentioning

confidence: 99%

Gene expression profiling identifies pathways involved in seeds maturation of Jatropha curcas

Maghuly

Deák

Vierlinger

et al. 2019

Preprint

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Background: Jatropha curcas, a tropical shrub, is a promising biofuel crop, which produces seeds with a high content of oil and protein. To better understand the development of its seeds to improve Jatropha`s agronomic performance, a two-step approach was performed: 1) generation of the entire transcriptome of six different maturation stages of J. curcas seeds using 454-Roche sequencing of a cDNA library, 2) comparison of transcriptional expression levels in six different developmental stages of seeds using a custom Agilent 8x60K oligonucleotide microarray. Results: A total of 793,875 high-quality reads were assembled into 19,841 unique full-length contigs, of which 13,705 could be annotated with Gene Ontology (GO) terms. Microarray data analysis identified 9,111 probes (out of 57,842 probes), which were differentially expressed between the six developmental stages. The expression results were validated for 70 randomly selected putative genes. Result from cluster analyses showed that transcripts related to sucrose, fatty acid, flavonoid, phenylpropanoid, lignin, hormone biosynthesis were over-represented in the early stage, while lipid storage, seed dormancy and maturation in the late stage. Generally, the expression of the most over-represented transcripts decrease in the last stage of seed maturation. Further, expression analyses of different maturation stages of J. curcas seed showed that most changes in transcript abundance occurred between the two last stages, suggesting that the timing of metabolic pathways during seed maturation in J. curcas is in late stages. The co-expression result showed a high degree of connectivity between genes that play essential role in fatty acid biosynthesis and nutrient mobilization. Furthermore, seed development and hormone pathways are significantly well connected. Conclusion: The obtained results revealed DESs regulating important pathways related to seed maturation, which could contribute to understanding the complex regulatory network during seed development. This study provides detailed information on transcription changes during J. curcas seed development and provides a starting point for a genomic survey of seed quality traits. The current results highlighted specific genes and processes relevant to the molecular mechanisms involved in Jatropha seed development, and it is anticipated that this data can be delivered to other Euphorbiaceae species of economic value.

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Relationship between fruit maturation stage and physiological quality of physic nut (Jatropha curcas L.) seeds

Cited by 19 publications

References 12 publications

Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas

Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas

Does the Maturity ofJatropha CurcasL. Affect the Quality and Quantity of the Yield of Oil for Biodiesel Production?

Gene expression profiling identifies pathways involved in seeds maturation of Jatropha curcas

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