Relationship between Embryo Collection Results after Superovulation Treatment of Japanese Black Cows and Their Plasma β-carotene and Vitamin Concentrations

Sekizawa, Fumio; Sawai, Ken; Tanaka, Minoru; Okuda, Kiyoshi

doi:10.1262/jrd.11-075h

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…In our experiment, hormone levels were not analyzed; however, it may be possible to infer that serum levels of anti-Müllerian hormone were similar in all animal groups, since, according to Rico et al (2012) and Sevgi et al (2019), higher concentrations of this hormone favor the production of embryos. The numbers of viable embryos in the animals of this study did not differ among the groups, with values similar to those observed by Sekizawa et al (2012), Takahashi et al (2013), andFacioli et al (2020), when conducting research on the same breed of animals used in this study.…”

Section: Discussionsupporting

confidence: 85%

“…The total number of embryos was 2.88±0.85 in females in which uterine flushing was done with RL, 3.00±1.23 in those with RL + 1% FBS, and 4.57±1.72 in those in which D-PBS was used (Table 3), resulting in a mean of 3.48 embryos/female, lower than the results obtained by An et al (2016) (Sekizawa et al, 2012) and total structures of 10.00±7.6 and 4.5±3.9 of viable structures (Takahashi et al, 2013).…”

Section: Discussionmentioning

confidence: 68%

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Comparison of cattle embryo collection between Ringer’s lactate solution and Dulbecco’s phosphate-buffered saline

Moraes¹,

Braganholo²,

Cavalieri³

et al. 2021

Revista Brasileira De Zootecnia

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The objective of this study was to determine the efficiency of Ringer's lactate solution (RL) and RL + 1% fetal bovine serum (FBS) and compare them with the efficiency of Dulbecco's phosphate-buffered saline (D-PBS). Twenty-two Wagyu female cattle were subjected to superovulation and were randomly distributed to form three groups: group 1 -uterine flushing with RL (n = 8), group 2 -uterine flushing with RL + 1% FBS (n = 7), and group 3 -uterine flushing with D-PBS (n = 7, control group). Cows received a CIDR ® device containing 1.9 g of progesterone at random stages of the estrous cycle (day 0). Progesterone withdrawal occurred on day 8 in the morning. For heifers, 160 mg of porcine follicle-stimulating hormone (FSH-P) was used and for cows, 200 mg. Prostaglandin F2α was also injected on the eighth day of FSH-P administration. On day 9, in the morning, hCG was administered. Females were superovulated and inseminated twice in a fixed time for embryo transfer. On the 16th day, females were subjected to uterine flushing for embryo collection. We collected 76 embryos from 22 females subjected to superovulation, of which 52 were transferable and 24 had degenerated. The total of embryos collected was 23, 16, and 23 for groups 1, 2, and 3, respectively. The embryo recovery rates per group were 13.86±4.23, 15.39±4.61 and 27.16±13.33%, in groups 1, 2, and 3, respectively. The means for the total structures collected per female were 2.88±0.85, 3.00±1.23, and 4.57±1.72 in groups flushed with RL, RL + 1% FBS, and D-PBS, respectively. We conclude that Ringer's lactate solution and Ringer's lactate solution + 1% of FBS and Dulbecco's phosphate-buffered saline showed no significant differences in terms of embryo quality or quantity, suggesting that Ringer's lactate solution is an alternative for collecting embryos in cattle.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 68%

Comparison of cattle embryo collection between Ringer’s lactate solution and Dulbecco’s phosphate-buffered saline

Moraes¹,

Braganholo²,

Cavalieri³

et al. 2021

Revista Brasileira De Zootecnia

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“…β -carotene, a precursor to vitamin A, has been postulated to contribute to follicular growth. A positive correlation has been observed between the plasma vitamin and β -carotene concentrations and the number of transferable embryos in vitro fertilization process ( Sekizawa et al, 2012 ). And, it has been proposed that β -carotene acts as an antioxidant in lipid phases by quenching singlet oxygen and scavenging the peroxyl radical ( Ikeda et al, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Raman Spectrum of Follicular Fluid: A Potential Biomarker for Oocyte Developmental Competence in Polycystic Ovary Syndrome

Huang

Chen

et al. 2021

Front. Cell Dev. Biol.

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Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in reproductive women where abnormal folliculogenesis is considered as a common characteristic. Our aim is to evaluate the potential of follicular fluid (FF) Raman spectra to predict embryo development and pregnancy outcome, so as to prioritize the best promising embryo for implantation, reducing both physiological and economical burdens of PCOS patients. In addition, the altered metabolic profiles will be identified to explore the aetiology and pathobiology of PCOS. In this study, follicular fluid samples obtained from 150 PCOS and 150 non-PCOS women were measured with Raman spectroscopy. Individual Raman spectrum was analyzed to find biologic components contributing to the occurrence of PCOS. More importantly, the Raman spectra of follicular fluid from the 150 PCOS patients were analyzed via machine-learning algorithms to evaluate their predictive value for oocyte development potential and clinical pregnancy. Mean-centered Raman spectra and principal component analysis (PCA) showed global differences in the footprints of follicular fluid between PCOS and non-PCOS women. Two Raman zones (993–1,165 cm−1 and 1,439–1,678 cm−1) were identified for describing the largest variances between the two groups, with the former higher and the latter lower in PCOS FF. The tentative assignments of corresponding Raman bands included phenylalanine and β -carotene. Moreover, it was found that FF, in which oocytes would develop into high-quality blastocysts and obtain high clinical pregnancy rate, were detected with lower quantification of the integration at 993–1,165 cm−1 and higher quantification of the integration at 1,439–1,678 cm−1 in PCOS. In addition, based on Raman spectra of PCOS FF, the machine-learning algorithms via the fully connected artificial neural network (ANN) achieved the overall accuracies of 90 and 74% in correctly assigning oocyte developmental potential and clinical pregnancy, respectively. The study suggests that the PCOS displays unique metabolic profiles in follicular fluid which could be detected by Raman spectroscopy. Specific bands in Raman spectra have the biomarker potential to predict the embryo development and pregnancy outcome for PCOS patients. Importantly, these data may provide some valuable biochemical information and metabolic signatures that will help us to understand the abnormal follicular development in PCOS.

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“…β -carotene, a precursor to vitamin A, has been postulated to contribute to follicular growth. A positive correlation has been observed between the plasma vitamin and β -carotene concentrations and the number of transferable embryos in vitro fertilization process [40]. And, it has been proposed that β -carotene acts as an antioxidant in lipid phases by quenching singlet oxygen and scavenging the peroxyl radical [41].…”

Section: Discussionmentioning

confidence: 99%

Raman Spectrum of Follicular Fluid: a Potential Biomarker for Oocyte Developmental Competence in Polycystic Ovary Syndrome

Huang

et al. 2021

Preprint

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Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in reproductive women where abnormal folliculogenesis is considered as a common characteristic. Our aim is to evaluate the potential of follicular fluid (FF) Raman spectra to predict oocyte development and pregnancy outcome, so as to prioritize the best promising oocyte for implantation, reducing both physiological and economical burdens of PCOS patients. In addition, the altered metabolic profiles will be identified to explore the aetiology and pathobiology of PCOS. Methods: In this study, follicular fluid samples obtained from 150 PCOS and 150 non-PCOS women were measured with Raman spectroscopy. Individual Raman spectrum was analyzed to find biologic components contributing to the occurrence of PCOS. More importantly, the Raman spectra of follicular fluid from the 150 PCOS patients were analyzed via machine-learning algorithms to evaluate their predictive value for oocyte development potential and clinical pregnancy. Results: Mean-centered Raman spectra and principal component analysis showed global differences in the footprints of follicular fluid between PCOS and non-PCOS women. Two Raman zones (993-1,165 cm-1 and 1,439-1,678cm-1) were identified for describing the largest variances between the two groups, with the former higher and the latter lower in PCOS FF. The tentative assignments of corresponding Raman bands included phenylalanine and β -carotene. Moreover, it was found that FF, in which oocytes would develop into high-quality blastocysts and obtain high clinical pregnancy rate, were detected with lower quantification of the integration at 993-1,165 cm-1 and higher quantification of the integration at 1,439-1,678 cm-1 in PCOS. In addition, based on Raman spectra of PCOS FF, the machine-learning algorithms via the fully connected artificial neural network (ANN) achieved the overall accuracies of 90% and 74% in correctly assigning oocyte developmental potential and clinical pregnancy, respectively. Conclusions: The study suggests that the PCOS displays unique metabolic profiles in follicular fluid which could be detected by Raman spectroscopy. Specific bands in Raman spectra have the biomarker potential to predict the oocyte development and pregnancy outcome for PCOS patients. Importantly, these data may provide some valuable biochemical information and metabolic signatures that will help us to understand the abnormal follicular development in PCOS.

show abstract

Relationship between Embryo Collection Results after Superovulation Treatment of Japanese Black Cows and Their Plasma β-carotene and Vitamin Concentrations

Cited by 6 publications

References 16 publications

Comparison of cattle embryo collection between Ringer’s lactate solution and Dulbecco’s phosphate-buffered saline

Comparison of cattle embryo collection between Ringer’s lactate solution and Dulbecco’s phosphate-buffered saline

Raman Spectrum of Follicular Fluid: A Potential Biomarker for Oocyte Developmental Competence in Polycystic Ovary Syndrome

Raman Spectrum of Follicular Fluid: a Potential Biomarker for Oocyte Developmental Competence in Polycystic Ovary Syndrome

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