Relationship between Dynamics of TNF-α and Its Soluble Receptors in Saliva and Periodontal Health State

Kibune, Ryota; Muraoka, Kosuke; Morishita, Masaki; Ariyoshi, Wataru; Awano, Shuji

doi:10.3390/dj10020025

Cited by 10 publications

(7 citation statements)

References 28 publications

(39 reference statements)

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“…Porphyromonas gingivalis, one of the representative periodontal disease pathogens in oral bacteria, can produce lipopolysaccharide (LPS) endotoxins that can stimulate the induction of inflammatory cytokines and contribute to the process of alveolar bone loss and connective tissue destruction [8][9][10]. In addition, the secretion of various inflammatory cytokines including tumor necrosis factor-alpha (TNF-α) generated by periodontal pathogen stimulation can act as a major factor in periodontal tissue destruction [11,12]. In particular, TNF-α is a factor that can induce osteoclast differentiation in macrophages.…”

Section: Introductionmentioning

confidence: 99%

Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells

Lee¹,

Lee²,

Lee³

et al. 2023

CIMB

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Periodontitis, a disease caused by inflammation of oral bacteria, contributes to the loss of alveolar bone and destruction of connective tissues. Porphyromonas gingivalis, a Gram-negative bacterium, is known to possess important pathogenic factors for periodontal disease. In this study, we investigated the anti-periodontitis effects of Magnolia kobus extract (MKE) and magnolin as a component of Magnolia kobus (MK) in murine macrophage RAW 264.7 cells stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS). Effects of MKE and magnolin on the mechanism of RAW 264.7 cellular inflammation were determined by analyzing nitric oxide (NO) production and Western blot protein expression (n = 3). MKE/magnolin inhibited NO production without affecting cell survival. MKE/magnolin treatment inhibited LPS-induced pro-inflammatory cytokines, expression levels of matrix metalloproteinases (MMPs such as MMP-1, 3, 8, 9, and 13), and protein levels of inflammatory mediators (such as TNF-α, IL-1β, and mPGES-1). MKE/magnolin also suppressed NF-κB activation by inhibiting the TLR4 signaling pathway. These findings suggest that MKE has a therapeutic effect on inflammatory periodontal disease caused by oral bacterium P. gingivalis and that magnolin is a major functional component in the anti-inflammatory effect of MKE.

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Section: Introductionmentioning

confidence: 99%

Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells

Lee¹,

Lee²,

Lee³

et al. 2023

CIMB

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“…There are some strengths in our research. As studies examining periodontitis and salivary cytokines are usually conducted with smaller populations (about 100 participants or less) (Jentsch et al, 2017;Johnston et al, 2021;Kibune et al, 2022), the strength of our study is the larger sample size. In both studies, DILGOM 2007 and H2000, dietary information was gathered with the same validated FFQ (Männistö et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Dietary inflammatory index in relation to salivary cytokine concentrations and periodontitis: A cross‐sectional analysis

Syrjäläinen,

Männistö,

Könönen

et al. 2023

J Clinic Periodontology

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AimTo examine the associations of dietary inflammatory index (DII) with salivary cytokine concentrations and periodontitis after controlling for body mass index (BMI), socio‐demographic factors and lifestyle.Materials and MethodsSubgroups from two Finnish surveys, DILGOM 2007 and Health 2000, were included (total n = 727). The DII scores were calculated based on a food frequency questionnaire. Periodontal status was assessed with a cumulative risk score in DILGOM 2007 and by pocket depth measurement in Health 2000. From saliva, interleukin (IL)‐1β, IL‐1 receptor antagonist, IL‐6, IL‐8, IL‐10 and tumour necrosis factor (TNF)‐α concentrations were measured.ResultsThe DII scores did not differ between non‐periodontitis and periodontitis participants in pairwise comparison. After adjusting for energy intake, periodontal status, BMI, age, education level, smoking habit and physical activity, DII was not associated with salivary cytokine concentrations. After adjusting for salivary cytokine levels and other confounding factors, DII was associated with periodontitis in the Health 2000 subgroup but not in the DILGOM 2007 subgroup.ConclusionsThe current data support the evidence that diet is not associated with salivary cytokine levels but may be associated with periodontitis. The association observed between diet and periodontitis is related to factors other than diet‐dependent inflammatory tendency in the oral cavity.

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“…Pregnant women commonly experience diseases such as caries and periodontitis. Periodontitis causes an increase in the salivary-soluble TNF-α receptor, which leads to an increase in the TNF-α level [ 34 ]. An increase in the salivary TNF-α level in pregnant women, especially those who are obese, can affect the weight of the infant [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Oral Microbiome Profiles and Inflammation in Pregnant Women Who Used Orthodontic Appliances

et al. 2022

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It is common for women to undergo orthodontic treatment during pregnancy, especially through the use of fixed orthodontic devices. In changing the oral microbiome profile, it is crucial to increase the immune responses of pregnant women using fixed orthodontics; however, changes in the microbiomes of pregnant women with orthodontic appliances can be adjusted. Therefore, we aimed to conduct research on the oral cavity microbiome profiles, specifically IL-6 and TNF-α, of pregnant women using fixed orthodontic appliances. We proposed an observational analysis of 30 third-trimester pregnant women. OHI-S was recorded, saliva collection was performed using the passive drool method for IL-6 and TNF-α, and analysis and mucosal swabs were used to determine the oral microbiome profile. Kruskal–Wallis and post hoc Bonferroni tests were used to identify any significant differences with values of p < 0.05. Of these pregnant women, those with orthodontic appliances developed 10 types of bacteria at similar levels (>80%) from the genera Streptococcus, Lactobacillus, and Veillonella. There was no difference between the oral microbiomes of the control group and the pregnant women with a history of orthodontic appliance use. While the level of TNF-α in the women with orthodontic appliances was higher compared with the control group who had never used orthodontic appliances (p < 0.05), there was no difference in the IL-6 levels. The IL-6 and microbiome profile produced normal results, so the use of orthodontic appliances during pregnancy should be allowed with conditions. Pregnant women with orthodontic appliances must keep the oral cavity clean and their appliances well-maintained to avoid oral problems.

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Relationship between Dynamics of TNF-α and Its Soluble Receptors in Saliva and Periodontal Health State

Cited by 10 publications

References 28 publications

Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells

Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells

Dietary inflammatory index in relation to salivary cytokine concentrations and periodontitis: A cross‐sectional analysis

Oral Microbiome Profiles and Inflammation in Pregnant Women Who Used Orthodontic Appliances

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