Relationship between astaxanthin production and the intensity of anabolic processes in the yeast Phaffia rhodozyma

Vustin, M. M.; Belykh, E N; Kishilova, S A

doi:10.1007/s11021-005-0004-0

Cited by 29 publications

(26 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in yeast cultures on HS medium lower cell yields of total carotenoids were produced, with the yield of astaxanthin reduced by 50% depending on the strain. Vustin et al [2004] indicated that the initial C/N ratio below 5 has a negative effect on the synthesis of carotenoids in X. dendrorhous, so poor growth of studied yeasts, low values of Y X/S and low yields of astaxanthin recorded in this study in cultures on HS might be the result from the defi cit of some compounds, e.g. specifi c amino acids, macro-and microelements or vitamins.…”

Section: Development Of Yeasts and Production Of Carotenoids On Test mentioning

confidence: 62%

Astaxanthin synthesis by Xanthophyllomyces dendrorhous DSM 5626 and its astaxanthin overproducing mutants on xylose media under diferent illumination

Stachowiak

2014

Acta Sci Pol Technol Aliment

View full text Add to dashboard Cite

Background. Astaxanthin is the most important and expensive carotenoid pigment used in aquaculture. Its commercial attractiveness is also related with its antioxidant potential. Xanthophyllomyces dendrorhous yeast is considered to be promising for commercial production of astaxanthin. The aim of this study was to investigate the possibility of the growth and astaxanthin production by X. dendrorhous strains on media containing xylose under different illumination. Material and methods. X. dendrorhous DSM 5626 and its mutants: 10BE and 26UV were used in this study. The cultures were carried out on hydrolysed rye stillage (HS) and YM medium with xylose (YM-K). Cell concentration, total carotenoid and astaxanthin yields were assessed in 5-day cultures. The effect of illumination in the range of 0-5,000 lx on growth and on astaxanthin production of yeasts in cultures run on YM-K medium was also examined. Results. For the tested yeast strains better growth parameters and astaxanthin yields were obtained on the YM-K medium, on which for all strains the highest pigment yields were recorded at 600-1,000 lx. The highest concentration of astaxanthin in cells was recorded for 26UV in a culture at 1,000 lx (0.51 g·kg -1 DCW). The volume yield of the pigment regardless of strain was highest in cultures at 600 lx. In this case 10BE was found to be the best astaxanthin producer with a yield of 2.15 mg·dm -3 . Conclusions. Astaxanthin synthesis in X. dendrorhous DSM 5626 and its mutants was better on YM-K medium comparing to hydrolysed rye stillage. Moreover, carotenogenesis in the studied yeast strains was subjected to marked photoregulation. Illumination within the range of 600-1,000 lx promotes carotenogenesis and astaxanthin production, while exceeding a certain light capacity results in microbial cell death.

show abstract

Section: Development Of Yeasts and Production Of Carotenoids On Test mentioning

confidence: 62%

Astaxanthin synthesis by Xanthophyllomyces dendrorhous DSM 5626 and its astaxanthin overproducing mutants on xylose media under diferent illumination

Stachowiak

2014

Acta Sci Pol Technol Aliment

View full text Add to dashboard Cite

show abstract

“…Results in Table 2 show that reduction of the nitrogen content stimulated the production of carotenoids (and increased the value of the C:N ratio). Vustin et al (29) found that a C:N ratio above 5 improves the biosynthesis of carotenoids. However, this ratio, by itself, cannot explain cell growth and carotenoid production.…”

Section: Carotenoid Production Kineticsmentioning

confidence: 99%

“…However, this ratio, by itself, cannot explain cell growth and carotenoid production. Other nutrients, such as vitamins, amino acids and minerals, and the conditions of the process (temperature, pH, oxygen content) exert strong infl uence on the metabolism of yeasts (29,30). On the other hand, the trials with C:N ratio under 5 had the worst performance (trials 7, 9 and 11).…”

Section: Carotenoid Production Kineticsmentioning

confidence: 99%

“…Carotenoid production kinetics determined by the Plackett -Burman design at 25 °C and 150 rpm: a and b) biomass concentration, c and d) specifi c production of carotenoids (μg/g), e and f) volumetric production of carotenoids (μg/mL), and g and h) pH carotenogenesis can be stimulated by nitrogen limitation. Vustin et al (29) suggested that nitrogen in excess in the medium culture can increase cell growth, but suppress the activity of enzymes in carotenoid conversion.…”

Section: Eff Ect Of the Culture Medium Composition On Carotenoid Prodmentioning

confidence: 99%

See 1 more Smart Citation

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

Silva¹,

Borba²,

Kalil³

et al. 2016

Food Technol. Biotechnol.

View full text Add to dashboard Cite

SummarySearch for naturally grown food has stimulated the biotechnological production of carotenoids. Therefore, the use of the yeast Xanthophyllomonas dendrorhous has been researched due to its abilities to assimilate diff erent sources as substrates and to produce high amounts of carotenoids. Furthermore, alternative sources have been used as the culture medium to reduce costs and environmental impact. A potent carotenoid is astaxanthin in view of its health-promoting and antioxidative properties. It consists of diff erent geometrical isomers with trans and cis confi guration. In X. dendrorhous this carotenoid is mostly found in the trans form, but cis isomers can also be found. Carotenoid production was investigated in culture medium containing by-products such as raw glycerol (from biodiesel) and parboiled rice effl uent. The eff ects of the culture medium components on biomass concentration and specifi c and volumetric productions of carotenoids were verifi ed by the Plackett -Burman design. Cultivations were carried out with yeast Xanthophyllomonas dendrorhous NRRL Y-17268 at 25 °C and 150 rpm for 168 h. In this study, maximum production of carotenoids was obtained under the following conditions (in g/L): raw glycerol 10, glucose 10, yeast extract 10, malt extract 10 and peptone 1 at pH=6. Resulting specifi c and volumetric productions of carotenoids were 326.8 and 4.1 μg/g, respectively.

show abstract

“…This behavior is most likely associated with the composition of the culture medium, specifically the ratio of carbon to nitrogen (C:N), which was very low in the RPW medium (C:N = 0.05). According to Vustin, Belykh and Kishilova (2004), a C:N ratio less than 5 may influence the synthesis of carotenoids by P. rhodozyma in a negative way, whereas higher ratios indicate a smaller concentration of nitrogen in the culture medium, which causes a large reduction in biomass growth and thereby in the volumetric production of carotenoids. The alga H. pluvialis responded similar to the observations of this work, with nitrogen limitation in the medium culture resulting in the reduction of primary metabolism (synthesis of protein and chlorophyll) and affecting the secondary metabolism and biosynthesis of astaxanthin (Ip;Chen, 2005).…”

Section: Cultivation With Rice Parboiling Wastewatermentioning

confidence: 99%

RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma

et al. 2015

View full text Add to dashboard Cite

The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL -1 (259.1 μg g -1 ) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2 IV 6-2 fractional factorial design sequenced by a 2 4 central composite design). The conditions for maximizing the production of carotenoids were (g L -1) malt extract (16.25), peptone (8.75), sucrose (15) and rice parboiling wastewater (87.5), with a pH of 5 at 25 °C and 150 rpm for 144 h, which produced a concentration 5.3 μg mL -1 (628.8 μg g -1 ).Index terms: Biodye; submerged cultive; response surface methodology; yeast. RESUMOO interesse pela produção de carotenoides por fontes naturais é crescente, em decorrência da possibilidade de atuar como corante e sua capacidade antioxidante biológica potente. Neste estudo, avaliou-se o uso da água de parboilização do arroz, como substrato alternativo para a bioprodução de carotenoides, usando a levedura Phaffia rhodozyma que, como única fonte de nutrientes, foi promissora, alcançando 0,6 µg mL -1 (259,1 µg g -1

show abstract

Relationship between astaxanthin production and the intensity of anabolic processes in the yeast Phaffia rhodozyma

Cited by 29 publications

References 15 publications

Astaxanthin synthesis by Xanthophyllomyces dendrorhous DSM 5626 and its astaxanthin overproducing mutants on xylose media under diferent illumination

Astaxanthin synthesis by Xanthophyllomyces dendrorhous DSM 5626 and its astaxanthin overproducing mutants on xylose media under diferent illumination

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma

Contact Info

Product

Resources

About