Relationship analysis between gene expression profiles and rat liver cirrhosis occurrence

Abstract

Liver cirrhosis (LC) is a kind of liver disease which is pathologically characterized by abnormality and necrosis of hepatic cells, proliferation of fibrous tissue, nodular regeneration and pseudolobule formation. To explore the mechanism of LC occurrence at the level of mRNA, Rat Genome 230 2.0 Array was used to detect gene expression profiles of rat fibrotic livers in 3, 6 and 9 weeks after CCl 4 treatment in this study. It was found that a total of 305 genes including 153 up-regulated, 150 down-regulated and 2 up/down-regulated genes, were related to LC occurrence. Then, k-means clustering was employed to classify above 305 genes into 5 clusters based on gene expression similarity, and EASE analysis further indicated that the above genes were mainly associated with metabolic process, stress reaction, cell growth and apoptosis/death. Thereafter, ingenuity pathway analysis (IPA) software was used to analyze potential effects of the above-mentioned 305 genes, and the results suggested that lipid metabolism and cell growth were inhibited while cell apoptosis/death was activated, but immune/inflammatory response was first activated and then inhibited. Furthermore, IPA also predicted that several signal pathways "ERK/MAPK Signaling", "p38 MAPK", "Endothelin-1 Signaling", "Growth Hormone Signaling", "LPS/IL-1-mediated inhibition of RXR function" and "IL-6 Signaling" were involved in regulating the occurrence of liver cirrhosis. It was concluded that 305 genes and 3 kinds of physiological activities were closely related to LC occurrence.

Liver transcriptomic and plasma metabolomic profiles of fattening lambs are modified by feed restriction during the suckling period1

“…Also, our results are similar to previous studies in humans describing that retarded early growth is strongly related to high plasma concentrations of the hemostatic factors fibrinogen and factor VII (Barker et al, 1992), and thus to a more severe inflammatory response (Spurlock, 1997). Additionally, the lower expression of TF in RES lambs might be related to increased inflammation in these animals (Ritchie et al, 1999), which seems to be corroborated by the higher expression of the GUCY2C gene (Wang et al, 2017). These results suggest that, compared with the ADL lambs, the RES lambs might have responded in a different way to hepatic proinflammatory stimulus, potentially expending more energy towards combating systemic inflammation and redirecting less nutrients towards growth and protein accretion (Paradis et al, 2015).…”

Programación metabólica de la eficiencia alimentaria en corderos: efecto de la restricción de alimento en la etapa de lactancia y mecanismos moleculares implicados = Metabolic programming of lamb feed efficiency: effect of feed restriction during the suckling period and molecular mechanisms involved

“…Also, our results are similar to previous studies in humans describing that retarded early growth is strongly related to high plasma concentrations of the hemostatic factors fibrinogen and factor VII (Barker et al, 1992), and thus to a more severe inflammatory response (Spurlock, 1997). Additionally, the lower expression of TF in RES lambs might be related to increased inflammation in these animals (Ritchie et al, 1999), which seems to be corroborated by the higher expression of the GUCY2C gene (Wang et al, 2017). These results suggest that, compared with the ADL lambs, the RES lambs might have responded in a different way to hepatic proinflammatory stimulus, potentially expending more energy towards combating systemic inflammation and redirecting less nutrients towards growth and protein accretion .…”