Relation Between Viruses From Acute Gastroenteritis of Children and Newborn Calves

Flewett, T. H.; Bryden, A.S.; Davies, Heather A.; Woode, G. N.; Bridger, Janice C.; Derrick, JoannaM

doi:10.1016/s0140-6736(74)91631-6

Cited by 277 publications

(188 citation statements)

References 8 publications

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“…It has been shown that CF and FA tests can detect group specific antigen, probably situated within the inner capsid layer and common to all the known rotaviruses (Flewett et al 1974;Woode et al 1976;Thouless et al 1977). Human serum antibody titres against rotavirus infection were studied by FA (0rstavik, Figenschau, Haug & Ulstrup, 1976;Blacklow, Echeverria & Smith, 1976), and CFT (Kapikian et al 1975;Blacklow et al 1976).…”

Section: Introductionmentioning

confidence: 99%

Distribution and titres of rotavirus antibodies in different age groups

Elias

1977

J. Hyg.

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SUMMARYThree hundred and fifty-seven sera selected at random from hospital patients of all ages were examined for rotavirus antibodies using indirect immunofluorescence (FA) and complement fixation tests (CFT). Three hundred and fourteen of these were also tested for neutralizing antibodies to human rotavirus. Sera from patients admitted with a diagnosis of acute gastroenteritis were excluded from this survey.FA antibodies were found in newborn infants but fell to undetectable titres at 3 months. The highest titres were found in children between the ages of one and three years. In older age groups, the modal titre fell gradually with increasing age until, in sera from those above 70 years of age, FA antibodies were almost undetectable. The same pattern was observed with neutralizing antibodies. A high modal titre of CF antibodies was only found in sera from those aged one to three years.

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Section: Introductionmentioning

confidence: 99%

Distribution and titres of rotavirus antibodies in different age groups

Elias

1977

J. Hyg.

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“…The reovirus-like particles, which have been seen in faeces of young children with acute nonbacterial gastroenteritis, have been found to be morphologically indistinguishable from those of neonatal calf diarrhoea [8,101. The calf and human viruses also appeared morphologically and antigenically distinct from both reoviruses and orbiviruses, and the term rotavirus has been proposed [7-91. Although the calf and human rotaviruses are morphologically indistinguishable, we were unable to infect calves with human virus.…”

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confidence: 99%

Gnotobiotic Piglets Experimentally Infected with Neonatal Calf Diarrhoea Reovirus-Like Agent (Rotavirus)

et al. 1976

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Abstract. Gnotobiotic piglets were infected with a strain of rotavirus that had been isolated from a calf and passaged four times in piglets. The resulting disease was studied by light and electron microscopy at 21,44 and 68h after inoculation. In thesmall intestine infection caused desquamation of the epithelial cells of the villi resulting in severe stunting. There was severe damage to microvilli and accumulation of lipid within the cytoplasm. Virus particles were seen in epithelial cells covering the stunted villi. Infection also caused desquamation of the superficial epithelial cells of the stomach mucosa and of the epithelial cells of the bronchi, bronchioles, and alveoli of the lungs. The reovirus-like particles, which have been seen in faeces of young children with acute nonbacterial gastroenteritis, have been found to be morphologically indistinguishable from those of neonatal calf diarrhoea [8, 101. The calf and human viruses also appeared morphologically and antigenically distinct from both reoviruses and orbiviruses, and the term rotavirus has been proposed [7-91.Although the calf and human rotaviruses are morphologically indistinguishable, we were unable to infect calves with human virus. Piglets, however, are susceptible to infection with human virus [5] but do not become ill. We passed calf rotavirus serially five times in gnotobiotic piglets, and each passage produced profuse diarrhoea, depression and anorexia. Our serological studies have shown that pigs are naturally infected either with calf

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“…Rotaviruses are characterized by a distinctive morphology, which led Flewett et al [1] to recognize them as separate from reo-and orbiviruses. In contrast to reo-and orbiviruses, each rotavirus particle is thought to contain 11 segments of double-stranded RNA that form a characteristic pattern on polyacrylamide gel electrophoresis.…”

Section: Cells and Virusesmentioning

confidence: 99%

Detection of Porcine Rotavirus From Tissue and Faecal Specimens

Prabha

Verghese

2009

Indian Journal of Medical Microbiology

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Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute diarrhoea. The purpose of this study was to evaluate the porcine intestinal tissue scaffold for Rotavirus and to study the incidence of this virus among pig herds. Only one isolate was successfully adapted to grow in cell line MA 104 from faecal samples. This isolate was further confirmed by reverse transcriptase polymerase chain reaction and sequence analysis.

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Relation Between Viruses From Acute Gastroenteritis of Children and Newborn Calves

Cited by 277 publications

References 8 publications

Distribution and titres of rotavirus antibodies in different age groups

Distribution and titres of rotavirus antibodies in different age groups

Gnotobiotic Piglets Experimentally Infected with Neonatal Calf Diarrhoea Reovirus-Like Agent (Rotavirus)

Detection of Porcine Rotavirus From Tissue and Faecal Specimens

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