Detection of Porcine Rotavirus From Tissue and Faecal Specimens

Prabha, S; Verghese, S

doi:10.4103/0255-0857.49430

Cited by 7 publications

(4 citation statements)

References 10 publications

(8 reference statements)

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“…Worldwide reports and surveys have identified G3, G4, G5 and G11 as the most common G genotypes and P[6] and P[7]) as the most common P genotypes associated with diarrhoea in pigs. Also rare genotypes (G1, G2-like, G6, G8, G9, G10, and G12) were reported in pigs, which are commonly associated with humans and cattle (ESTES, 2001;GOUVEA et al, 1994;MARTELLA et al, 2005;MATTHIJNSSENS et al, 2008;PAPP et al, 2013;PRABHA and VERGHESE, 2009;RAMOS et al, 1998;STEELE et al, 2004;VARGHESE et al, 2006). Reports on Rotavirus prevalence from the Indian subcontinent revealed that the most common G types (G1-4) and P types (P[4] and P[8]) globally account for three quarters of all strains on the subcontinent (MALIK et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of Genogroup 5 Rotavirus associated with piglet diarrhoea in the North East Region of India

Kylla¹,

Dutta²,

Roychoudhury³

et al. 2018

Vet. arhiv

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Rotaviruses have been recognized as an important etiological agent of non-bacterial acute gastroenteritis in young children and animals of several species worldwide, including diarrhoea in weaning and post-weaning piglets. In this study, we report the prevalence and molecular epidemiology of rotaviruses detected from piglets in different regions of the northeastern hilly region of India. A total of 457 faecal samples (339 diarrhoeal and 118 non-diarrhoeal) were collected from piglets from local (n = 130) and cross breed (n = 327) piglets between July 2013 to June 2015 in different seasons of the year. All the samples were subjected to RNA-PAGE and RT-PCR analysis. Rotaviruses were detected in 4.81% animals by RNA-PAGE and 7.43% animals by RT-PCR, with the highest prevalence (9.67%) from Meghalaya state. All the isolates were recorded as GARV and genogroup 5. The prevalence was higher in unorganized farms (10.77%) compared to organized farms (4.0%) with higher detection from diarrhoeic (9.14%) compared to non-diarrhoeic animals (2.54%). A higher prevalence was also recorded during the summer (12.5%) and winter (9.09%) seasons. On the basis of the sequence analysis, all the isolates were placed in a unique single cluster, different from other Indian isolates from humans and animals, which were in close proximity with human isolates. This is the first report of the detection of G5 Rotavirus associated with piglet diarrhoea in India.

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Section: Discussionmentioning

confidence: 99%

Detection and characterization of Genogroup 5 Rotavirus associated with piglet diarrhoea in the North East Region of India

Kylla¹,

Dutta²,

Roychoudhury³

et al. 2018

Vet. arhiv

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“…PRoV is a double-stranded, nonenveloped RNA virus belonging to the Reoviridae family. All three viruses cause an acute and highly contagious enteric disease characterized by severe enteritis, watery diarrhea, vomiting, and dehydration that is associated with a high degree of mortality in suckling piglets (Coussement et al, 1982;Laude et al, 1990;Prabha and Verghese, 2009).…”

Section: Introductionmentioning

confidence: 99%

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

Zp¹,

Yang²,

Wd³

et al. 2016

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Summary. -Piglet diarrhea epidemics result in major economic losses for the swine industry. Four viruses are closely linked to porcine diarrhea: porcine kobuvirus (PKV), porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PRoV). We have conducted an epidemiology study to determine the frequency of infection and co-infection with these viruses in China, and characterized the genetic variation of the isolated PEDV and PKV strains. Stool and intestinal samples (n = 314) were collected from piglets with diarrhea in China from years 2012 to 2014. RT-PCR was used to detect PKV, PEDV, TGEV, and PRoV. Phylogenetic relationships between reference strains and the isolated PEDV and PKV strains were determined based on the M and 3D gene sequence. Th e rates of infection with PKV, PEDV, TGEV and PRoV were 29.9%, 24.2%, 1.91%, and 0.31%, respectively. Co-infections with PKV and the other three viruses were very common. Co-infection of PKV and PEDV was detected in 15.0% (47/314) of the samples. Phylogenetic analysis of the PKV 3D gene indicated that there were some phylogenetic diff erences in the PKV strains across regions within China. However, according to the PEDV M gene, strains clustered into three groups and the primary group was distinct from the vaccine strain CV777. Th is study provides insights in to the prevalence of diarrhea viruses and their prevention and control in China.Keywords: porcine diarrhea viruses; co-infection; porcine kobuvirus; porcine epidemic diarrhea virus; porcine gastroenteritis; porcine rotavirus * Corresponding authors: E-mail: wenjiejin1@163.com, aijian@ yzu.edu.cn; phone: +8613815809571.# Th ese authors contributed equally to this work. Abbreviations: PKV = porcine kobuvirus; PEDV = porcine epidemic diarrhea virus; TGEV = porcine transmissible gastroenteritis virus; ProV = porcine rotavirus

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“…Rotaviruses are generally species specific, but crossspecies transmission is possible, as has been demonstrated experimentally. Several case studies have indicated infection of humans by animal rotaviruses (Prabha and Verghese 2009). Thus, there is an urgent requirement of probiotic strains for treating and preventing the infectious diarrhea caused by enteric viruses and microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Bile tolerant Lactobacillus reuteri isolated from pig feces inhibits enteric bacterial pathogens and porcine rotavirus

Seo

Mun

et al. 2010

Vet Res Commun

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Lactic acid producing bacterial strain Probio-16 was isolated from the swine excrements under anaerobic conditions and characterized by morphology and biochemical characteristics. The strain was further identified by 16S rRNA gene sequencing and phylogeneitc analysis. The antimicrobial activity of the strain was assayed by testing for growth inhibition of thirteen pathogenic microorganisms. The strain was tested for antiviral activity against porcine rotavirus in vitro in African green monkey epithelial cell line TF-104. Antibiotic susceptibility of the strain against 13 antibiotics was tested using disk diffusion method. Phenotypically and through 16S rRNA gene sequences, Probio-16 was identified and named as Lactobacillus reuteri Probio-16. This strain was resistant to pH 2.0, 5% porcine bile and exhibited antimicrobial activity against all the thirteen enteric bacterial pathogens tested. Probio-16 supernatant inhibited porcine rotavirus in vitro in TF-104 cell lines. Except for erythromycin and penicillin G at a concentration of 4 microg/ml, Probio-16 showed resistance to all other thirteen antibiotics tested. This study indicates L. reuteri Probio-16 as a novel strain with its tolerance to low pH and bile, antimicrobial activity, antibiotic resistance and antiviral activity against rotavirus, and an ideal probiotic candidate for animal and human application after the proper in vivo experiments.

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Detection of Porcine Rotavirus From Tissue and Faecal Specimens

Cited by 7 publications

References 10 publications

Detection and characterization of Genogroup 5 Rotavirus associated with piglet diarrhoea in the North East Region of India

Detection and characterization of Genogroup 5 Rotavirus associated with piglet diarrhoea in the North East Region of India

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

Bile tolerant Lactobacillus reuteri isolated from pig feces inhibits enteric bacterial pathogens and porcine rotavirus

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