Detection and characterization of Genogroup 5 Rotavirus associated with piglet diarrhoea in the North East Region of India

Kylla, Hosterson; Dutta, Tapan Kumar; Roychoudhury, P.; Mandakini, Rajkumari; Subudhi, Prashant K.

doi:10.24099/vet.arhiv.170613a

Cited by 7 publications

(7 citation statements)

References 30 publications

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“…Detection of Salmonella by real-time PCR The result of RT-PCR detection of salmonella showed that the salmonella was detected in 8 (8%) out of 100(100%) stool specimens that collected from patient with acute diarrhea. this result matched to fi ndings of Kylla et al 13 that show A total of 8.31% Salmonella were identifi ed with higher prevalence. Also, another study showed that the prevalence of Salmonella isolates was 6.9% Children aged between 1 to 3 years were test for all positive RT-PCR stool samples.…”

Section: The Results Of Rt-pcr Detection Of Campylobactersupporting

confidence: 90%

Evaluation of Molecular and Culture Method for Detection of Campylobacter, Salmonella, E. coli and Shigella in children infected with Diarrhea

Saife D. Al-Ahmer,

Musa K. Al-Janabi,

Iman M. Aufi

2021

Indian Journal of Forensic Medicine & Toxicology

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Acute diarrhea is the second most common cause of children deaths worldwide. Bacterial pathogens have been confirmed as the major cause of acute diarrhea among children. In this study, 100 stool samples were investigated from pediatric children with acute diarrheal illness aged from 1 month to 5 years old. Bacterial investigated was performed for the common enteric pathogens: Campylobacter, Salmonella, Shigella, and Diarrheagenic Escherichia coli included Microscopy, culture and confirmatory identification by biochemical reactions included API 20E system. Finally, the real time PCR investigated for these enteric bacteria. The overall prevalence of the pathogens for Campylobacter, Salmonella, Shigella and Diarrheagenic E. coli were 9%, 8%, 3% and 14%, respectively. The results showed significant association of the clinical symptoms with Shigella and Salmonella infection. The RT-PCR markedly improves the detection rates of bacterial stool pathogens and offers culture methods.

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Section: The Results Of Rt-pcr Detection Of Campylobactersupporting

confidence: 90%

Evaluation of Molecular and Culture Method for Detection of Campylobacter, Salmonella, E. coli and Shigella in children infected with Diarrhea

Saife D. Al-Ahmer,

Musa K. Al-Janabi,

Iman M. Aufi

2021

Indian Journal of Forensic Medicine & Toxicology

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“…Lack of scientific literature regarding the prevalence of EPEC; prevented us from comparing our results to precedents. However, similar findings were reported in a prevalence study of diarrhoeagenic bacterial and viral pathogens ( 17 , 18 ).…”

Section: Discussionsupporting

confidence: 89%

Characterisation of porcine enteropathogenic Escherichia coli isolated in northeastern India

Kylla

Dutta

Roychoudhury

et al. 2020

Journal of Veterinary Research

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IntroductionEnteropathogenic Escherichia coli (EPEC) is one of the main pathotypes causing gastroenteritis, particularly in young immunocompromised hosts. The study reports the prevalence, characterisation, and molecular epidemiology of EPEC from piglets in northeastern India.Material and MethodsA total of 457 faecal samples were collected, from which 1,286 E. coli strains were isolated and screened by PCR. The resultant EPEC strains were serotyped and phenotypically characterised for resistance against 15 antimicrobials. Also, the phylogenetic sequence was analysed for 11 selected strains.ResultsA total of 42 strains (3.26%) belonged to atypical EPEC, of which, 15 (35.71%, and 2.29% of the 654 strains from this farm type) were isolated from organised and 27 (64.29%, and 4.27% of the 632 strains from this farm type) from unorganised farms; further, 5 (11.90% of the EPEC strains and 1.51% of the 330 strains from this breed) were isolated from the indigenous breeds and 37 (88.10%, and 3.87% of the 956 strains from this breed) from crossbred piglets. Serogroups O111 (11.9%) and O118 (7.14%) were the most prevalent of the 10 present. Sequence analysis of a length of the eaeA gene of 11 isolates of the region showed them to have 100% homology with each other and their identity ranged from 99.4% to 99.7% with GenBank reference sequences. All the EPEC isolates were multi-drug resistant, showing the highest resistance to amoxicillin (80.9%) and cephalexin (76.19%).ConclusionThe study highlighted the association of EPEC with piglet’s diarrhoea in northeastern India. EPEC isolates belonged to many serotypes and phenotypically all were multi-drug resistant with close genetic homology.

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“…Many researchers [38][39][40][41] reported that RVA is the most common cause of viral diarrhea in neonatal pigs compared to Type C, and other workers have detected porcine Rotavirus in diarrheic fecal samples in nursing, weaning, and post-weaning pigs either alone or in combination of other enteric pathogens such as E. coli, Salmonella, and Adenovirus [42,43]. Rotavirus A is only often detected as the sole infectious agent than as coinfection in cases of neonatal diarrhea [31,44].…”

Section: Discussionmentioning

confidence: 99%

Coinfection of diarrheagenic bacterial and viral pathogens in piglets of Northeast region of India

Kylla¹,

Dutta

Roychoudhury

et al. 2019

Vet World

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Aim: This study aimed to study the prevalence of the coinfection of enteric bacterial and viral pathogens, namely Escherichia coli, Salmonella, Rotavirus, and Picobirnavirus from fecal samples of pre-weaned piglets in Northeast region of India. Materials and Methods: A total of 457 fresh fecal samples were collected from piglets under 9 weeks old during 2013-2015 from organized (n=225) and unorganized (n=232) farms of Manipur, Meghalaya, Mizoram, and Nagaland. Samples were collected from diarrheic (n =339) and non-diarrheic (n=118) piglets including local indigenous (n=130) and crossbreed (n=327) piglets in different seasons during the study period. The samples were processed for the isolation of E. coli and Salmonella and detection of their putative virulence genes by polymerase chain reaction (PCR). Samples were also processed for the detection of Rotavirus and Picobirnavirus by RNA-polyacrylamide agarose gel electrophoresis and reverse transcriptase-PCR (RT-PCR). Results: A total of 11 (2.40%) samples were found positive for two or more coinfecting enteric bacterial and viral pathogens. All the 11 positive fecal samples were recovered from diarrheic piglets. Salmonella Typhimurium (enterotoxin, stn gene) and Picobirnavirus genogroup 1 were found to be more frequent as coinfecting agents. Coinfection was recorded higher in unorganized (3.87%) compared to organized farm (0.88%). Again, higher detection was recorded in crossbreed (2.75%) than local indigenous piglets (1.53%). The occurrence of coinfection was found to be more common during summer (4.68%) followed by winter (2.27%) season. Conclusion: The present study highlighted the significance of E. coli, Salmonella, Rotavirus, and Picobirnavirus as important diarrheagenic pathogens causing coinfection in piglets in Northeast region of India. Probably, this is the first systematic study of the coinfection of four important diarrheagenic bacterial and viral agents associated with piglet diarrhea in India.

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Detection and characterization of Genogroup 5 Rotavirus associated with piglet diarrhoea in the North East Region of India

Cited by 7 publications

References 30 publications

Evaluation of Molecular and Culture Method for Detection of Campylobacter, Salmonella, E. coli and Shigella in children infected with Diarrhea

Evaluation of Molecular and Culture Method for Detection of Campylobacter, Salmonella, E. coli and Shigella in children infected with Diarrhea

Characterisation of porcine enteropathogenic Escherichia coli isolated in northeastern India

Coinfection of diarrheagenic bacterial and viral pathogens in piglets of Northeast region of India

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