Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Brown, Allan H.; Weis, Dale S.

doi:10.1104/pp.34.3.224

Cited by 67 publications

(21 citation statements)

References 11 publications

(14 reference statements)

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“…However, other studies show that the turnover of intermediates of the tricarboxylic acid cycle is unaffected by light in some unicellular green algae (33,34). Partial inhibition of respiratory CO. release in the light is not necessarily accompanied by a reduction in 0, uptake as would be expected if photoinhibition of respiration were mediated through photosynthetic ATP (4,48).…”

Section: Discussionmentioning

confidence: 90%

The Kok Effect in Chlamydomonas reinhardi

Healey

Myers

1971

Plant Physiol.

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A Haxo-Blinks rate-measuring oxygen electrode together with a modulated light source gave an average current signal (change in net Oa exchange) and a modulated current signal (photosynthetic 02 evolution). Using this apparatus, net 02 exchange and photosynthetic 02 evolution at low intensities have been studied in the green alga, Chlamydomonas reinhardi. At both 645 nm and 695 nm, the curves of net 02 exchange as a function of light intensity were steeper at lowest intensities than about compensation, indicative of the Kok effect. The effect was greater at 695 nm than at 645 nm. The corresponding curves of photosynthetic 02 evolution, on the other hand, showed no Kok effect; here, the slope was lowest at lowest intensity. The absence of the Kok effect in 02 evolution, together with its sensitivity to monofluoroacetic acid, show that it is due to an interaction of photosynthesis and respiration. The effect was exaggerated by 3-(3,4-dichlorophenyl (24) and of Jones and Myers (27) show that preferential activation of photosystem I exaggerates the Kok effect. Activation of system I in excess of activation of system II could depress respiratory 02 uptake in at least two ways. Hoch et al. (24) proposed that cyclic phosphorylation driven by system I could raise the ratio of cellular ATP to ADP sufficiently to restrict respiratory electron transport. Alternately, preferential activation of system I could shift the flow of respiratory reductant away from respiratory electron transport and into system I of photosynthetic electron transport, as suggested by Jones and Myers (27). Recent observations on anaerobic, DCMU3-resistant "4CO2 fixation by Chlamydomonas reinhardi (47), and on photoevolution of H2 by Scenedesmus obliquus (45) and Chlamydomonas moewusii (18) show that the latter pathway may operate in anaerobic cells. The observation of a marked Kok effect in C. moewusii and C. reinhardi led to a study of the phenomenon in the latter alga in an attempt to gain a fuller understanding of its mechanism. MATERIALS AND METHODS

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Section: Discussionmentioning

confidence: 90%

The Kok Effect in Chlamydomonas reinhardi

Healey

Myers

1971

Plant Physiol.

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“…Values for CO2 evolution were decreased by 30 to 50 % and those for 02 uptake were increased nearly 100 %. Thus, the work of Brown and Weis (3,23) indicates a dual effect of light on respiration and other metabolic processes associated with 02 uptake and CO. evolution. Hoch, Owens and Kok (10) recently have investigated the effects of light intensity on 0, release and 02 consumption of Anacystis nidulans and Scenedesmitus sp.…”

Section: Introductionmentioning

confidence: 99%

Effects of Light and Darkness on Gaseous Exchange of Bean Leaves

1964

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“…A few measurements have been done based on labeled carbon additions to closed systems containing single phytoplankton species (Brown and Weis 1959;Weger et al 1988;Xue et al 1996). Here we applied this approach to natural planktonic populations, evaluating its usefulness and limitations, and also testing the common assumption that O 2 consumption in the dark can be used to estimate CO 2 release in the light.…”

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confidence: 99%

Measurement of planktonic CO₂ respiration in the light

Carvalho

Eyre

2012

Limnology & Ocean Methods

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Respiration is an essential physiological process for all organisms and is a significant component of the global carbon cycle. However, CO 2 release rates in the light (CRR) by aquatic communities have seldom been measured directly; instead, the typical procedure is to measure oxygen consumption rates in the dark (OCR) and assume that they are approximately equivalent to CRR. Here we describe a method to measure CRR (and consequently gross photosynthesis rates) based on 13 C addition to incubation water in a closed system. We quantified planktonic CRR in situ in a lake, and also in laboratory experiments, where we tested the effects of temperature and irradiance on respiration rates. We also measured O 2 rates (respiration in the dark and net production in the light) to compare with those based on CO 2 , and found that in the lake, these rates were similar both for photosynthesis and respiration. In laboratory, however, they were very different, with CO 2 -based photosynthesis being higher, and CRR being lower than correspondent O 2 rates. These findings show that the usual approach of assuming similar OCR and CRR can be erroneous. We also found that higher temperature and irradiance caused higher CRR. The method was successful to provide CRR and photosynthetic measurements, but would greatly benefit from more precise techniques to measure the concentration of dissolved inorganic carbon in water.

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Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Cited by 67 publications

References 11 publications

The Kok Effect in Chlamydomonas reinhardi

The Kok Effect in Chlamydomonas reinhardi

Effects of Light and Darkness on Gaseous Exchange of Bean Leaves

Measurement of planktonic CO₂ respiration in the light

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Relation Between Respiration and Photosynthesis in the Green Alga, Ankistrodesmus braunii.

Cited by 67 publications

References 11 publications

The Kok Effect in Chlamydomonas reinhardi

The Kok Effect in Chlamydomonas reinhardi

Effects of Light and Darkness on Gaseous Exchange of Bean Leaves

Measurement of planktonic CO2 respiration in the light

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Measurement of planktonic CO₂ respiration in the light