Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis

Zhang, Bo; Kaneshi, Takuya; Ohta, Takao; Saku, Keijiro

doi:10.1194/jlr.m500192-jlr200

Cited by 35 publications

(35 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The different response to insulin treatment has been attributed to differences in the systemic inflammation level, which is higher in type 2 patients. This agrees with the finding that pre-diabetic insulin-resistant subjects with high systemic inflammation also have increased LDL(-) proportion (Zhang, 2005). Therefore, www.intechopen.com hyperglycemia would not promote the increase of LDL(-) directly but through an increase of systemic inflammation.…”

Section: Association Of Ldl(-) With Cardiovascular Risksupporting

confidence: 81%

“…This suggests that LDL(-) generation not only depends on lipid metabolism but also on other factors such as chronic inflammation. Both type 1 and type 2 diabetics have a high proportion of LDL(-) (Moro, 1998;SanchezQuesada, 1996;Sanchez-Quesada, 2001;Zhang, 2005). This would suggest that nonenzymatic glycosylation could be involved.…”

Section: Association Of Ldl(-) With Cardiovascular Riskmentioning

confidence: 99%

See 1 more Smart Citation

Modified Forms of LDL in Plasma

Sánchez-Quesada¹,

Villegas²

2012

Atherogenesis

View full text Add to dashboard Cite

Section: Association Of Ldl(-) With Cardiovascular Risksupporting

confidence: 81%

Section: Association Of Ldl(-) With Cardiovascular Riskmentioning

confidence: 99%

Modified Forms of LDL in Plasma

Sánchez-Quesada¹,

Villegas²

2012

Atherogenesis

View full text Add to dashboard Cite

“…We also found that the area under the non-modified LDL peak increased. Previous studies have shown that electronegative LDL is the most atherogenic component [14] and is associated with insulin resistance [15] and carotid artery atherosclerosis [16] in humans. Therefore, lipid profile analysis with cITP may be clinically useful for evaluating the effect of treatment with levothyroxin for the prevention of ACS in patients with secondary hyperlipidemia and hypothyroidism.…”

Section: Discussionmentioning

confidence: 99%

Effect of Levothyroxine on Total Lipid Profiles as Assessed by Analytical Capillary Isotachophoresis in a Patient with Hypothyroidism

et al. 2006

Self Cite

View full text Add to dashboard Cite

Abstract. The patient was a 51-year-old Japanese female who had been diagnosed with hyperlipidemia. At the first medical examination, her serum levels of total cholesterol (TC) and triglyceride (TG) were 482 and 205 mg/dl, respectively. Since hyperlipidemia was not improved by pravastatin, atorvastatin or niceritrol, and since the levels of thyroid-stimulating hormone (TSH) and free T4 were 730 IU/ml and 0.3 ng/dl, respectively, the patient was diagnosed as secondary hyperlipidemia with hypothyroidism. A method for the charge isolation of lipoproteins using capillary isotachophoresis (cITP) is proposed as a clinical application because it allows us to quantitatively measure electronegative low-density lipoprotein cholesterol (LDL-C), a potent marker of coronary heart disease. After 5 months of treatment with levothyroxine, Serum TC and LDL-C levels drastically decreased without statin treatment and high-density lipoprotein cholesterol (HDL-C) increased. In the lipoprotein profiles as assessed by cITP after treatment with levothyroxin, all HDL-C subfractions were increased and fast-migrating LDL/electronegative LDL appeared to be greatly reduced after treatment, while the area under the non-modified LDL peak was increased. The cITP analysis was able to obtain more information about coronary risk factors and may be clinically useful for evaluating the effect of treatment with levothyroxine in patients with hypothyroidism and secondary hyperlipidemia.

show abstract

“…It separates plasma lipoproteins into 8 fractions, comprising 3 high-density liporotein (HDL) fractions with fast (f), intermediate (I), and slow (s) electric mobility, a chylomicron/remnants fraction (fast very low-density lipoprotein (VLDL)), a VLDL/intermediate density lipoprotein (IDL) fraction (slow VLDL), 2 LDL fractions with fast and slow electric mobility, and a minor LDL fraction. [1][2][3][4][5] In our previous reports, the fast LDL fractions corresponded to electronegative LDL in which some oxidized LDL, VLDL, and small dense LDL etc, the so-called "bad cholesterol", are concentrated. 4 After the light LDL fraction is precipitated from whole serum using heparin-Mg 2+ , electronegative LDL in the small dense LDL fraction can be measured using cITP.…”

mentioning

confidence: 99%

“…4 After the light LDL fraction is precipitated from whole serum using heparin-Mg 2+ , electronegative LDL in the small dense LDL fraction can be measured using cITP. 4 In the present study we used this method before and after treatment with fenofibrate. …”

mentioning

confidence: 99%

Potent Capillary Isotachophoresis (cITP) for Analyzing a Marker of Coronary Heart Disease Risk and Electronegative Low-Density Lipoprotein (LDL) in Small Dense LDL Fraction

Noda

Zhang

Uehara

et al. 2005

Circ J

Self Cite

View full text Add to dashboard Cite

apillary isotachophoresis (cITP) is a newly established technique for characterizing plasma lipoprotein subfractions according to their electric charges. It separates plasma lipoproteins into 8 fractions, comprising 3 high-density liporotein (HDL) fractions with fast (f), intermediate (I), and slow (s) electric mobility, a chylomicron/remnants fraction (fast very low-density lipoprotein (VLDL)), a VLDL/intermediate density lipoprotein (IDL) fraction (slow VLDL), 2 LDL fractions with fast and slow electric mobility, and a minor LDL fraction. [1][2][3][4][5] In our previous reports, the fast LDL fractions corresponded to electronegative LDL in which some oxidized LDL, VLDL, and small dense LDL etc, the so-called "bad cholesterol", are concentrated. 4 After the light LDL fraction is precipitated from whole serum using heparin-Mg 2+ , electronegative LDL in the small dense LDL fraction can be measured using cITP. 4 In the present study we used this method before and after treatment with fenofibrate. Methods Quantification of Lipoprotein Subfractions by cITPcITP of serum lipoproteins was performed on a Beckman P/ACE MDQ system (Beckman-Coulter Inc, Tokyo, Japan) according to the method of Bottcher et al 6 with some modifications. All of the reagents used in the cITP analysis were purchased from Aldrich-Sigma (Tokyo, Japan) unless indicated otherwise. Dimethylpolysiloxane modified fused silica capillary (AT™-1) was purchased from Alltech Japan Inc (Tokyo, Japan). A 6 l sample of serum was diluted with 14 l of leading buffer consisting of 10 mmol/L HCl and 18 mmol/L ammediol (2-amino-2-methyl-1,3-propanediol) (pH 8.8), prestained with 10 l 0.1 mg/ml NBD C6-ceramide (Molecular Probes, Inc, OR, USA) for 5 min at room temperature, and mixed with 50 l of a mixture containing leading buffer with 0.35% hydroxypropylmethylcellulose, spacers, and 5-carboxy-fluorescein as an internal marker. The spacers included were the same as those described by Bottcher et al, including N-(2-acetamido)-2-aminoethanesulfonic acid, D-glucuronic acid, 1-octanesulfonic acid sodium salt, 3-(N-tris[hydroxymethyl]methylamino)-2-hydroxypropanesulfonic acid, N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, L-serine, L-glutamine, L-methionine, and glycine. The terminating buffer contained 24 mmol/L -alanine and 13 mmol/L ammediol, and was adjusted to pH 10.5 with saturated barium hydroxide solution. The sample was injected for 18 s at 20 pounds into a 30 cm-long capillary (inner diameter 180 m), and separation was performed at a constant 30 mA for 1 min and 10 kV for 7 min. The separated zones were monitored with argon-laser-induced fluorescence detection (excitation, 488 nm; emission, 520 nm). Each peak was identified and the peak area in relative fluorescence units was analyzed using 32 Karat Software version 5.0 (Beckman-Coulter Inc). The capillary was washed with leading buffer and water between runs. Separation of Light and Dense LDL Subfractions by Heparin-Mg 2+ PrecipitationThe light LDL subclass was separated from the dense LDL subclas...

show abstract

Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis

Cited by 35 publications

References 33 publications

Modified Forms of LDL in Plasma

Modified Forms of LDL in Plasma

Effect of Levothyroxine on Total Lipid Profiles as Assessed by Analytical Capillary Isotachophoresis in a Patient with Hypothyroidism

Potent Capillary Isotachophoresis (cITP) for Analyzing a Marker of Coronary Heart Disease Risk and Electronegative Low-Density Lipoprotein (LDL) in Small Dense LDL Fraction

Contact Info

Product

Resources

About